The efficacy and molecular mechanism of the effect of schisandrin b on the treatment of erectile dysfunction | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 4، دوره 22، شماره 8، آبان 2019، صفحه 866-871 اصل مقاله (507.42 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2019.27455.6701 | ||
| نویسندگان | ||
| WEI LIU* ؛ CHEN ZHAO؛ Yanping Huang؛ Yidong Liu؛ Mujun Lu | ||
| Department of Urology and Andrology, Renji Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200001, People’s Republic of China | ||
| چکیده | ||
| Objective(s): The purpose of this study is to determine the efficacy and molecular mechanism of the effect of schisandrin b (SCHB) on treating erectile dysfunction (ED) in a rat model with bilateral cavernous crushing nerve injury. Materials and Methods: The ED rat model was established with bilateral cavernous nerve crushing, and then confirmed by apomorphine. Fifty healthy eight-week-old ED rats were randomly assigned into five group, including control group (sham surgery), bilateral cavernous nerve crushing injury group (BCNC), BCNC with low SCHB (100 mg/d), BCNC with medium SCHB (200 mg/d) and BCNC with high SCHB (400 mg/d). For the last three groups, SCHB was given for 2 months. Then, we examined intracavernosal pressure (ICP), cyclic nucleotides (cAMP, cGMP), endothelial nitric oxide synthase (eNOS) and neuronal NOS (nNOS) in all groups. Results: In the study of ICP, SCHB was able to improve ED in a dose-dependent manner. In addition, as compared to the BCNC group, the relative expression of eNOS and nNOS in medium and high concentration of SCHB-treated groups are higher than BCNC group. Moreover, all groups treated with SCHB showed a significant higher expression level of cAMP and cGMP. Conclusion: These results suggested that SCHB were able to significantly improve the ED on rat model through the NO-cGMP and cAMP- protein kinase A (PKA) pathway. | ||
| کلیدواژهها | ||
| cAMP-PKA pathway؛ Cyclic nucleotides؛ Erectile dysfunction؛ Intracavernosum pressure؛ NO-cGMP pathway؛ Schisandrin b | ||
| مراجع | ||
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