Immunogenicity and protection effects of cationic liposome containing imiquimod adjuvant on leishmaniasis in BALB/c mice | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 12، دوره 22، شماره 8، آبان 2019، صفحه 922-931 اصل مقاله (785.64 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2019.35739.8515 | ||
| نویسندگان | ||
| Ahmad Mehravaran* 1، 2؛ Maryam Rezaei Nasab2؛ Hadi Mirahmadi1، 2؛ Iraj Sharifi3؛ Ebrahim Alijani4؛ Amin Reza Nikpoor5؛ Javad Akhtari6؛ Mansure Hojatizade7 | ||
| 1Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran | ||
| 2Department of Parasitology and Mycology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran | ||
| 3Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran | ||
| 4Clinical Immunology Research Center, Zahedan University of Medical Sciences, Zahedan, Iran | ||
| 5Immunogenetic and Cell Culture Department, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 6Immunogenetics Research Center, Department of Medical Nanotechnology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran | ||
| 7Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran | ||
| چکیده | ||
| Objective(s): Protection against leishmaniasis, in the murine model, is dependent on developing a potent CD4+ mediated Th1 type response. Liposomes can be applied as immunoadjuvants to stimulate immune responses to different antigens. In the present study, it was investigated whether DOTAP liposomes having SLA and imiquimod adjuvant, can induce a Th1 response and protect against Leishmania major challenge in BALB/c mice. Materials and Methods: Liposomes were provided applying the lipid film procedure. BALB/C mice were subcutaneously immunized, three times with 2-week intervals, with various formulations. Assessment of lesion development and parasite burden in the foot and spleen after challenge with L. major, assessment of Th1 cytokine (IFN-γ), and titration of IgG isotypes assessed the type of generated immune reaction and the protection extent. Results: The mice immunized with Liposome DOTAP+imiquimod+SLA showed smaller footpad swelling which was meaningfully different (P<0.05) compared with other groups. The highest level of IgG2a was observed with Lip DOTAP+imiquimod+SLA more than the control (P<0.001). Mice immunized with Lip DOTAP+SLA+imiquimod demonstrated the least number of live parasites in the footpad and spleen. Cytokine assay showed that the greatest IFN- γ secretion was seen in the splenocytes of mice immunized with all formulations as compared to the control group (P<0.0001). In contrast, the lowest IL-4 production was detectable in Lip+imiquimod+SLA spleen, which was not significantly different compared with other groups. Conclusion: The results of this study show that liposome DOTAP+SLA+imiquimod formulation generates a cellular immune response that is protective against challenge against L. major. | ||
| کلیدواژهها | ||
| Cationic Liposome؛ Leishmania major؛ Imiquimod؛ Immune Response؛ Vaccine | ||
| مراجع | ||
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