Metabolomics analysis of the saliva in patients with chronic hepatitis B using nuclear magnetic resonance: a pilot study | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 10، دوره 22، شماره 9، آذر 2019، صفحه 1044-1049 اصل مقاله (652 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2019.36669.8733 | ||
| نویسندگان | ||
| Kambiz Gilany1، 2؛ Ashraf Mohamadkhani3؛ Saeed Chashmniam4؛ Parisa Shahnazari1؛ Mehdi Amini1؛ Babak Arjmand5؛ Reza Malekzadeh3؛ Bibi Fatemeh Nobakht Motlagh Ghoochani* 6، 7 | ||
| 1Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran | ||
| 2Integrative Oncology Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran | ||
| 3Digestive Diseases Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran | ||
| 4Department of Chemistry, Sharif University of Technology, Tehran, Iran | ||
| 5Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran | ||
| 6Noncommunicable Diseases Research Center, Neyshabur University of Medical Sciences, Neyshabur, Iran | ||
| 7Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran | ||
| چکیده | ||
| Objective(s): Hepatitis B virus infection causes chronic disease such as cirrhosis and hepatocellular carcinoma. The metabolomics investigations have been demonstrated to be related to pathophysiologic mechanisms in many disorders such as hepatitis B infection. The aim of this study was to investigate the saliva metabolic profile of patients with chronic hepatitis B infection and to identify underlying mechanisms as well as potential biomarkers associated with the disease. Materials and Methods: Saliva from 16 healthy subjects and 20 patients with chronic hepatitis B virus were analyzed by nuclear magnetic resonance (NMR). Then, multivariate statistical analysis was performed to identify discriminative metabolites between two groups. Results: A set of metabolites were detected, including propionic acid, putrescine, acetic acid, succinic acid, tyrosine, lactic acid, butyric acid, pyruvic acid, 4-pyridoxic acid and 4-hydroxybenzoic acid, which in combination with one another could accurately distinguish patients from healthy controls. Our results clearly demonstrated altered metabolites are involved in nine metabolic pathways. Conclusion: Metabolomics has the potential to be considered as a novel clinical tool for hepatitis B diagnosis while contributing to a comprehensive understanding of disease mechanisms. | ||
| کلیدواژهها | ||
| Diagnostic biomarkers؛ Hepatitis B virus؛ Metabolomics؛ NMR؛ Saliva | ||
| مراجع | ||
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