سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Kozehkonan, Gholamreza Savari, Salehi, Majid, Farzamfar, Saeed, Ghanbari, Hossein, Adabi, Mehdi, Amani, Amir. (1398). Preparation and characterization of PCL polymeric scaffolds coated with chitosan/ bioactive glass/gelatin nanoparticles using the tips methodology for bone tissue engineering. , 6(4), 311-320. doi: 10.22038/nmj.2019.06.000009
Gholamreza Savari Kozehkonan; Majid Salehi; Saeed Farzamfar; Hossein Ghanbari; Mehdi Adabi; Amir Amani. "Preparation and characterization of PCL polymeric scaffolds coated with chitosan/ bioactive glass/gelatin nanoparticles using the tips methodology for bone tissue engineering". , 6, 4, 1398, 311-320. doi: 10.22038/nmj.2019.06.000009
Kozehkonan, Gholamreza Savari, Salehi, Majid, Farzamfar, Saeed, Ghanbari, Hossein, Adabi, Mehdi, Amani, Amir. (1398). 'Preparation and characterization of PCL polymeric scaffolds coated with chitosan/ bioactive glass/gelatin nanoparticles using the tips methodology for bone tissue engineering', , 6(4), pp. 311-320. doi: 10.22038/nmj.2019.06.000009
Kozehkonan, Gholamreza Savari, Salehi, Majid, Farzamfar, Saeed, Ghanbari, Hossein, Adabi, Mehdi, Amani, Amir. Preparation and characterization of PCL polymeric scaffolds coated with chitosan/ bioactive glass/gelatin nanoparticles using the tips methodology for bone tissue engineering. , 1398; 6(4): 311-320. doi: 10.22038/nmj.2019.06.000009

Preparation and characterization of PCL polymeric scaffolds coated with chitosan/ bioactive glass/gelatin nanoparticles using the tips methodology for bone tissue engineering

Nanomedicine Journal
مقاله 9، دوره 6، شماره 4، دی 2019، صفحه 311-320    اصل مقاله (745.43 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22038/nmj.2019.06.000009
نویسندگان
Gholamreza Savari Kozehkonan1؛ Majid Salehi2؛ Saeed Farzamfar3؛ Hossein Ghanbari1؛ Mehdi Adabi1؛ Amir Amani* 4، 5
1Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
2Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
3Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
4Department of Advanced Sciences and Technologies, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
5Medical Biomaterials Research Center (MBRC), Tehran University of Medical Sciences, Tehran, Iran
چکیده
Objective(s): The present study aimed to prepare polycaprolactone (PCL) scaffolds with high porosity and pore interconnectivity, in order to copy the microstructure of natural bones using the thermally induced phase separation (TIPS) technique.
Materials and Methods: The scaffolds were coated with chitosan (CH), bioactive glass (BG), and gelatin nanoparticles (GEL NPs) and assessed using scanning electron microscopy and Fourier-transform infrared spectroscopy (FTIR).
Results: The size of the prepared BG and GEL NPs was estimated to be 400 and 234 nanometers, respectively. The porosity and contact angle of PCL/CH/GEL NPs/BG was 74% and 72°, respectively. Weight loss and electron microscopy evaluations indicated the improved degradation rate of the scaffolds and spreading tendency of the cells on the scaffolds when modified as compared to the scaffolds that were purely obtained from PCL. In addition, the in-vitro studies revealed that the MG-63 cells cultured on the PCL/CH/GEL NPs/BG scaffolds showed improved cell proliferation more significantly compared to the scaffolds obtained from PCL, PCL/CH/GEL NPs, PCL/CH, and PCL/GEL NPs. Mechanical examinations also showed that PCL/CH/GEL/BG scaffolds had the highest mechanical strength compared to other groups (i.e., 4.66 Mpa). Cell viability was estimated to be 96.7%, and the alizarin red test indicated the significant improvement of mineralization in the PCL/CH/GEL NP group.
Conclusion: According to the results, the PCL scaffolds that were modified by CH/GEL NPs/BG had the high potency to be used as bone tissue engineering scaffolds.
کلیدواژه‌ها
Bioactive Glass؛ Chitosan؛ Gelatin NPs؛ PCL؛ Thermally Induced Phase Separation
مراجع

1.Caballero M, Jones DC, Shan Z, Soleimani S, van Aalst JA. Tissue engineering strategies to improve osteogenesis in the juvenile swine alveolar cleft model. Tissue Eng. 2017; 23(12): 889-899.
2.Nicholas JG, Watkins LE, Voytik-Harbin SL. Bone tissue engineering: scalability and optimization of densified collagen-fibril bone graft substitute materials. The Summer Undergraduate Research Fellowship (SURF) Symposium. 2016. USA.
3.Deepthi S, Venkatesan J, Kim S-K, Bumgardner JD, Jayakumar R. An overview of chitin or chitosan/nano ceramic composite scaffolds for bone tissue engineering. Int J Biol Macromol. 2016; 93: 1338-1353.
4.Biswas D, Tran P, Tallon C, O’Connor A. Combining mechanical foaming and thermally induced phase separation to generate chitosan scaffolds for soft tissue engineering. J Biomater Sci Polym Ed. 2017; 28(2): 207-26.
5.Salehi M, Naseri‐Nosar M, Ebrahimi‐Barough S, Nourani M, Khojasteh A, Hamidieh AA, Amani A, Farzamfar S, Ai J. Sciatic nerve regeneration by transplantation of Schwann cells via erythropoietin controlled‐releasing polylactic acid/multiwalled carbon nanotubes/gelatin nanofibrils neural guidance conduit. J Biomed Mater Res B Appl Biomater. 2017; 106(4): 1463-1476.
6.Kasoju N, Kubies D, Sedlačík T, Janoušková O, Koubková J, Kumorek MM, Rypáček F. Polymer scaffolds with no skin-effect for tissue engineering applications fabricated by thermally induced phase separation. Biomed Mater. 2016;11(1): 015002.
7.Langford CR, Cameron NR. Materials for Tissue Engineering and 3D Cell Culture. In Bruns N, Kilbinger A (eds) Bio-inspired Polymers, Royal Society of Chemistry; 2016. p. 460-89.
8.Rose FR, Oreffo RO. Bone tissue engineering: hope vs hype. Biochem Biophys Res Commun. 2002; 292(1): 1-7.
9.Venkatesan J, Kim S-K. Nano-hydroxyapatite composite biomaterials for bone tissue engineering—a review. J Biomed Nanotechnol. 2014;10(10): 3124-3140.
10.Kamath MS, Ahmed SS, Dhanasekaran M, Santosh SW. Polycaprolactone scaffold engineered for sustained release of resveratrol: therapeutic enhancement in bone tissue engineering. Int J nanomed. 2014; 9: 183.
11.Chen C-H, Lee M-Y, Shyu VB-H, Chen Y-C, Chen C-T, Chen J-P. Surface modification of polycaprolactone scaffolds fabricated via selective laser sintering for cartilage tissue engineering. Mater Sci Eng C Mater Biol Appl. 2014 ;40: 389-397.
12.Yazdimamaghani M, Razavi M, Vashaee D, Pothineni VR, Rajadas J, Tayebi L. Significant degradability enhancement in multilayer coating of polycaprolactone-bioactive glass/gelatin-bioactive glass on magnesium scaffold for tissue engineering applications. Appl Surf Sci. 2015; 338: 137-45.
13.Naseri-Nosar M, Farzamfar S, Sahrapeyma H, Ghorbani S, Bastami F, Vaez A, Salehi M. Cerium oxide nanoparticle-containing poly (ε-caprolactone)/gelatin electrospun film as a potential wound dressing material: In vitro and in vivo evaluation. Mater Sci Eng C Mater Biol Appl. 2017; 81: 366-372.
14.LogithKumar R, KeshavNarayan A, Dhivya S, Chawla A, Saravanan S, Selvamurugan N. A review of chitosan and its derivatives in bone tissue engineering. Carbohydr polym. 2016; 151: 172-188.
15.Tan H, Wu J, Lao L, Gao C. Gelatin/chitosan/hyaluronan scaffold integrated with PLGA microspheres for cartilage tissue engineering. Acta Biomater. 2009; 5(1): 328-337.
16.Li X, Jin L, Balian G, Laurencin CT, Anderson DG. Demineralized bone matrix gelatin as scaffold for osteochondral tissue engineering. Biomaterials. 2006; 27(11): 2426-2433.
17.Liu X, Smith LA, Hu J, Ma PX. Biomimetic nanofibrous gelatin/apatite composite scaffolds for bone tissue engineering. Biomaterials. 2009; 30(12): 2252-2258.
18.Gönen SÖ, Taygun ME, Küçükbayrak S. Fabrication of bioactive glass containing nanocomposite fiber mats for bone tissue engineering applications. Compos Struct. 2016; 138: 96-106.
19.Fu Q, Saiz E, Rahaman MN, Tomsia AP. Bioactive glass scaffolds for bone tissue engineering: state of the art and future perspectives. Mater Sci Eng C Mater Biol Appl. 2011; 31(7): 1245-1256.
20.Naseri-Nosar M, Salehi M, Hojjati-Emami S. Cellulose acetate/poly lactic acid coaxial wet-electrospun scaffold containing citalopram-loaded gelatin nanocarriers for neural tissue engineering applications. Int J Biol Macromol. 2017; 103 :701-708.
21.Nosar MN, Salehi M, Ghorbani S, Beiranvand SP, Goodarzi A, Azami M. Characterization of wet-electrospun cellulose acetate based 3-dimensional scaffolds for skin tissue engineering applications: influence of cellulose acetate concentration. Cellulose. 2016; 23(5): 3239-3248.
22.Lien S-M, Ko L-Y, Huang T-J. Effect of pore size on ECM secretion and cell growth in gelatin scaffold for articular cartilage tissue engineering. Acta biomater. 2009 ;5(2): 670-679.
23.Murphy CM, Haugh MG, O’Brien FJ. The effect of mean pore size on cell attachment, proliferation and migration in collagen–glycosaminoglycan scaffolds for bone tissue engineering. Biomaterials. 2010; 31(3): 461-466.
24.Azarmi S, Huang Y, Chen H, McQuarrie S, Abrams D, Roa W, Finlay WH, Miller GG, Lobenberg R. Optimization of a two-step desolvation method for preparing gelatin nanoparticles and cell uptake studies in 143B osteosarcoma cancer cells. J Pharm Pharm Sci. 2006; 9(1): 124-132.
25.Gautam S, Chou C-F, Dinda AK, Potdar PD, Mishra NC. Fabrication and characterization of PCL/gelatin/chitosan ternary nanofibrous composite scaffold for tissue engineering applications. J Mater Sci. 2014; 49(3): 1076-1089.
26.Meskinfam M, Sadjadi M, Jazdarreh H. Biomimetic preparation of nano hydroxyapatite in gelatin-starch matrix. Eng Technol. 2011; 52: 395-398.
27.Lari A, Sultana N. Chitosan coated and non-coated composite scaffolds based on poly(caprolactone)(PCL) and hydroxyapatite (HA). ARPN J Eng Appl Sci. 2016; 11: 7.
28.Tsakalof A, Manoudis P, Karapanagiotis I, Chryssoulakis I, Panayiotou C. Assessment of synthetic polymeric coatings for the protection and preservation of stone monuments. J Cult Herit. 2007; 8(1): 69-72.
29.Van Wachem P, Beugeling T, Feijen J, Bantjes A, Detmers J, Van Aken W. Interaction of cultured human endothelial cells with polymeric surfaces of different wettabilities. Biomaterials. 1985; 6(6): 403-408.
30.Qian Y, Zhang Z, Zheng L, Song R, Zhao Y. Fabrication and characterization of electrospun polycaprolactone blended with chitosan-gelatin complex nanofibrous mats. J Nanomater. 2014; 2014: 1.
31.Li Y, Ceylan M, Shrestha B, Wang H, Lu QR, Asmatulu R, Yao L. Nanofibers support oligodendrocyte precursor cell growth and function as a neuron-free model for myelination study. Biomacromolecules. 2013; 15(1): 319-326.
32.Sung H-J, Meredith C, Johnson C, Galis ZS. The effect of scaffold degradation rate on three-dimensional cell growth and angiogenesis. Biomaterials. 2004; 25(26): 5735-5742.
33.Salehi M, Naseri Nosar M, Amani A, Azami M, Tavakol S, Ghanbari H. Preparation of pure PLLA, pure chitosan, and PLLA/chitosan blend porous tissue engineering scaffolds by thermally induced phase separation method and evaluation of the corresponding mechanical and biological properties. Int J Polym Mater Poly Biomaterials. 2015; 64(13): 675-682.
34.Díaz E, Sandonis I, Valle MB. In vitro degradation of poly (caprolactone)/nHA composites. J Nanomater. 2014; 2014: 185.
35.Liu H, Slamovich EB, Webster TJ. Less harmful acidic degradation of poly (lactic-co-glycolic acid) bone tissue engineering scaffolds through titania nanoparticle addition. Int J Nanomed. 2006; 1(4): 541.
36.Thuaksuban N, Nuntanaranont T, Pattanachot W, Suttapreyasri S, Cheung LK. Biodegradable polycaprolactone-chitosan three-dimensional scaffolds fabricated by melt stretching and multilayer deposition for bone tissue engineering: assessment of the physical properties and cellular response. Biomed Mater. 2011; 6(1): 015009.
37.Yin H-M, Qian J, Zhang J, Lin Z-F, Li J-S, Xu J-Z, Li ZM. Engineering porous poly (lactic acid) scaffolds with high mechanical performance via a solid state extrusion/porogen leaching approach. Polymers. 2016; 8(6): 213.
38.Karageorgiou V, Kaplan D. Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials. 2005; 26(27): 5474-591.
39.Wan Y, Xiao B, Dalai S, Cao X, Wu Q. Development of polycaprolactone/chitosan blend porous scaffolds. J Mater Sci Mater Med. 2009; 20(3): 719-724.
40.Eshraghi S, Das S. Mechanical and microstructural properties of polycaprolactone scaffolds with 1-D, 2-D, and 3-D orthogonally oriented porous architectures produced by selective laser sintering. Acta Biomater. 2010; 6(7): 2467.
41.Chan B, Leong K. Scaffolding in tissue engineering: general approaches and tissue-specific considerations. Eur Spine J. 2008; 17(4): 467-479.
42.Fabbri P, Cannillo V, Sola A, Dorigato A, Chiellini F. Highly porous polycaprolactone-45S5 Bioglass® scaffolds for bone tissue engineering. Compos Sci Technol. 2010; 70(13): 1869-1878.
43.Prabhakaran MP, Venugopal J, Ramakrishna S. Electrospun nanostructured scaffolds for bone tissue engineering. Acta Biomater. 2009; 5(8): 2884-2893.

 

آمار
تعداد مشاهده مقاله: 3,867
تعداد دریافت فایل اصل مقاله: 3,075