سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Emamgholi, Asgar, Rahimi, Mohsen, seyyed Tabaei, Seyyed Javad, Ghorbani, Teymoor, Ziai, Seyed Ali, Brouki Milan, Peiman, Khodadoust, Mahdi, Keshavarzian, Nafiseh. (1398). Investigation of osteoblast-like cells cultured on nano-hydroxyapatite/chitosan based composite scaffold in the treatment of bone defects and limited mobility. , 6(3), 167-175. doi: 10.22038/nmj.2019.06.00003
Asgar Emamgholi; Mohsen Rahimi; Seyyed Javad seyyed Tabaei; Teymoor Ghorbani; Seyed Ali Ziai; Peiman Brouki Milan; Mahdi Khodadoust; Nafiseh Keshavarzian. "Investigation of osteoblast-like cells cultured on nano-hydroxyapatite/chitosan based composite scaffold in the treatment of bone defects and limited mobility". , 6, 3, 1398, 167-175. doi: 10.22038/nmj.2019.06.00003
Emamgholi, Asgar, Rahimi, Mohsen, seyyed Tabaei, Seyyed Javad, Ghorbani, Teymoor, Ziai, Seyed Ali, Brouki Milan, Peiman, Khodadoust, Mahdi, Keshavarzian, Nafiseh. (1398). 'Investigation of osteoblast-like cells cultured on nano-hydroxyapatite/chitosan based composite scaffold in the treatment of bone defects and limited mobility', , 6(3), pp. 167-175. doi: 10.22038/nmj.2019.06.00003
Emamgholi, Asgar, Rahimi, Mohsen, seyyed Tabaei, Seyyed Javad, Ghorbani, Teymoor, Ziai, Seyed Ali, Brouki Milan, Peiman, Khodadoust, Mahdi, Keshavarzian, Nafiseh. Investigation of osteoblast-like cells cultured on nano-hydroxyapatite/chitosan based composite scaffold in the treatment of bone defects and limited mobility. , 1398; 6(3): 167-175. doi: 10.22038/nmj.2019.06.00003

Investigation of osteoblast-like cells cultured on nano-hydroxyapatite/chitosan based composite scaffold in the treatment of bone defects and limited mobility

Nanomedicine Journal
مقاله 3، دوره 6، شماره 3، مهر 2019، صفحه 167-175    اصل مقاله (656.49 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22038/nmj.2019.06.00003
نویسندگان
Asgar Emamgholi1؛ Mohsen Rahimi* 2؛ Seyyed Javad seyyed Tabaei* 2؛ Teymoor Ghorbani3؛ Seyed Ali Ziai4؛ Peiman Brouki Milan1؛ Mahdi Khodadoust3؛ Nafiseh Keshavarzian5
1Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
2Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
4Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
چکیده
Objective(s): Design and construction of biocompatible and biodegradable scaffolds are among the main goals of tissue engineering. Recently, use of nano-hydroxyapatite as a bioactive bioceramic agent with high similarity to the mineral phase of the human bone tissue, in combination with biodegradable polymers and implant coatings has attracted the attention of researchers in the field of biomaterial sciences. The present study aimed to assess the differentiation of bone marrow stromal cells (BMSCs) in osteoblast-like cells on the chitosan/polyethylene oxide (PEO)/nano-hydroxyapatite scaffold in mature rats.
Materials and Methods: Chitosan and PEO solution with the weight ratio of 80:20 and 70:30 were prepared, and 2% weight of nano-hydroxyapatite was added. Nanofibers were prepared using the electrospinning method, and the morphology was studied using scanning electron microscopy (SEM). Afterwards, the BMSCs of mature rats were cultured on nanofibers and differentiated by adding a differentiation medium. The survival of the differentiated cells was evaluated at the end of the first, second, and third week using acridine orange staining, and the morphology of the differentiated cells exposed to nanofibers was assessed using SEM.
Results: The mean diameter of the nanofibers with the ratio of 80:20 was 150±17 nanometers. The differentiation of BMSCs into the osteoblast-like cells on nanofibers was confirmed using Alizarin red staining. The results indicated a significant decrease in the survival of the differentiated cells in the nanofiber groups by the end of the third week of differentiation compared to the control samples.
Conclusion: According to the results, BMSCs could be differentiated into osteoblast-like cells in the presence of the chitosan/PEO nanofibers containing nano-hydroxyapatite.
کلیدواژه‌ها
Bioceramics؛ Bone tissue engineering؛ chitosan؛ Nano-hydroxyapatite؛ Nanocomposites
مراجع

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