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Hoseinzadeh, Safoura, Raeisi, Elham, Lemoigne, Yves, Heidarian, Esfandiar. (1398). Effects of combined 5-Fluorouracil and ZnO NPs on human breast cancer MCF-7 Cells: P53 gene expression, Bcl-2 signaling pathway, and invasion activity. , 6(3), 232-240. doi: 10.22038/nmj.2019.06.000010
Safoura Hoseinzadeh; Elham Raeisi; Yves Lemoigne; Esfandiar Heidarian. "Effects of combined 5-Fluorouracil and ZnO NPs on human breast cancer MCF-7 Cells: P53 gene expression, Bcl-2 signaling pathway, and invasion activity". , 6, 3, 1398, 232-240. doi: 10.22038/nmj.2019.06.000010
Hoseinzadeh, Safoura, Raeisi, Elham, Lemoigne, Yves, Heidarian, Esfandiar. (1398). 'Effects of combined 5-Fluorouracil and ZnO NPs on human breast cancer MCF-7 Cells: P53 gene expression, Bcl-2 signaling pathway, and invasion activity', , 6(3), pp. 232-240. doi: 10.22038/nmj.2019.06.000010
Hoseinzadeh, Safoura, Raeisi, Elham, Lemoigne, Yves, Heidarian, Esfandiar. Effects of combined 5-Fluorouracil and ZnO NPs on human breast cancer MCF-7 Cells: P53 gene expression, Bcl-2 signaling pathway, and invasion activity. , 1398; 6(3): 232-240. doi: 10.22038/nmj.2019.06.000010

Effects of combined 5-Fluorouracil and ZnO NPs on human breast cancer MCF-7 Cells: P53 gene expression, Bcl-2 signaling pathway, and invasion activity

Nanomedicine Journal
مقاله 10، دوره 6، شماره 3، مهر 2019، صفحه 232-240    اصل مقاله (1.07 M)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22038/nmj.2019.06.000010
نویسندگان
Safoura Hoseinzadeh1؛ Elham Raeisi2؛ Yves Lemoigne3؛ Esfandiar Heidarian* 1
1Clinical Biochemistry Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
2Department of Medical Physics and Radiology, Shahrekord University of Medical Sciences, Shahrekord, Iran
3Institute for Medical Physics, Ambilly, France
چکیده
Objective(s): The significant contribution of nanoparticles to cancer treatment has attracted therapeutic attention. The present study aimed to evaluate the synergistic effects of 5-fluorouracil (5-FU) and zinc oxide nanoparticles (ZnO NPs) as multimodal drug delivery on human breast cancer MCF-7 cells.
Materials and Methods: In this in-vitro study, the impact of 5-FU and ZnO NPs in the single or combined forms was evaluated on cell viability, colony formation, apoptosis, p53 gene expression, and Bcl-2 signaling protein in MCF-7 breast cancer cell line using several techniques, such as MTT, clonogenic assay, flow cytometry, real-time quantitative polymerase chain reaction, and Western blot.
Results: In this study, 5-FU combined with ZnO NPs showed synergistic effects against MCF-7 within 48 hours. In addition, the combination of 5-FU and ZnO NPs at the respective concentrations of 1 µM and 45 µg/ml exhibited significant apoptosis (79.53%), p53 gene expression (3.6 folds), reduction of cell invasion (9.82%), and plating efficiency (5%), thereby leading to the significant reduction of cell viability (40±0.9%) and decreased Bcl-2 anti-apoptotic protein relative to untreated control cells.
Conclusion: According to the results, the synergistic effects of combined ZnO NPs and 5-FU on MCF-7 human breast cancer cells were exerted via Bcl-2 inhibition and the up-regulation of p53 expression.
کلیدواژه‌ها
Bcl-2؛ p53 expression؛ Zinc oxide naoparticles؛ 5-Fluorouracil
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