سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Shakibaie, Mojtaba, Alipour-Esmaeili-Anari, Fatemeh, Adeli-Sardou, Mahboubeh, Ameri, Atefeh, Doostmohammadi, Mohsen, Forootanfar, Hamid, Ameri, Alieh. (1398). Antibacterial and anti-biofilm effects of microwave-assisted biologically synthesized zinc nanoparticles. , 6(3), 223-231. doi: 10.22038/nmj.2019.06.00009
Mojtaba Shakibaie; Fatemeh Alipour-Esmaeili-Anari; Mahboubeh Adeli-Sardou; Atefeh Ameri; Mohsen Doostmohammadi; Hamid Forootanfar; Alieh Ameri. "Antibacterial and anti-biofilm effects of microwave-assisted biologically synthesized zinc nanoparticles". , 6, 3, 1398, 223-231. doi: 10.22038/nmj.2019.06.00009
Shakibaie, Mojtaba, Alipour-Esmaeili-Anari, Fatemeh, Adeli-Sardou, Mahboubeh, Ameri, Atefeh, Doostmohammadi, Mohsen, Forootanfar, Hamid, Ameri, Alieh. (1398). 'Antibacterial and anti-biofilm effects of microwave-assisted biologically synthesized zinc nanoparticles', , 6(3), pp. 223-231. doi: 10.22038/nmj.2019.06.00009
Shakibaie, Mojtaba, Alipour-Esmaeili-Anari, Fatemeh, Adeli-Sardou, Mahboubeh, Ameri, Atefeh, Doostmohammadi, Mohsen, Forootanfar, Hamid, Ameri, Alieh. Antibacterial and anti-biofilm effects of microwave-assisted biologically synthesized zinc nanoparticles. , 1398; 6(3): 223-231. doi: 10.22038/nmj.2019.06.00009

Antibacterial and anti-biofilm effects of microwave-assisted biologically synthesized zinc nanoparticles

Nanomedicine Journal
مقاله 9، دوره 6، شماره 3، مهر 2019، صفحه 223-231    اصل مقاله (687.99 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22038/nmj.2019.06.00009
نویسندگان
Mojtaba Shakibaie1، 2؛ Fatemeh Alipour-Esmaeili-Anari3؛ Mahboubeh Adeli-Sardou2، 4؛ Atefeh Ameri5؛ Mohsen Doostmohammadi5؛ Hamid Forootanfar* 1، 5؛ Alieh Ameri* 6
1Department of Pharmaceutical Biotechnology, School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran
2Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran
3Student Research Committee, School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran
4Department of Biotechnology, Institute of Science, High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
5Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
6Department of Medicinal Chemistry, School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran
چکیده
Objective(s): The present study aimed to investigate the antibacterial and anti-biofilm potential of the non-oxidized form of zinc nanoparticles (Zn NPs) prepared by a ‘green approach’ using the Lavandula vera extract with microwave irradiation.
Materials and Methods: After synthesis of Zn NPs, the microdilution and disk diffusion methods was applied for antimicrobial evaluation followed by anti-biofilm activity measurement using crystal violet colorimetric assay procedure.
Results: The obtained results demonstrated the production of spherical Zn NPs within the size range of 30-80 nanometers. The measured minimum inhibitory concentration of the Zn NPs and ZnSO4 against the biofilm-producing and clinically isolated pathogens of Staphylococcus aureus, Pseudomonas aeruginosa, and Proteus mirabilis was estimated to be more than 2560 µg/ml. In addition, a non-significant increase (P>0.05) was observed in the antibacterial activity against methicillin-resistant S. aureus after the addition of the Zn NPs (500 µg/disk) to the antibiotic discs containing tobramycin, erythromycin, tetracycline, azithromycin, and kanamycin compared to ZnSO4. On the other hand, the Zn NPs significantly decreased the biofilm formation of P. mirabilis compared to P. aeruginosa (P<0.05). Biofilm formation by S. aureus also reduced to 68.3±2.1% in the presence of the Zn NPs (640 µg/ml), which was considered significant compared to P. mirabilis and P. aeruginosa at the same concentration (P<0.05).
Conclusion: To sum up, the biofilm inhibitory activity of Zn NPs at higher concentrations than 160 µg/ml against S. aureus and P. mirabilis was more significant compared to the inhibitory effects of ZnSO4. However, further investigations are required in order to determine the antibacterial and anti-biofilm mechanism of Zn NPs.
کلیدواژه‌ها
Antibacterial Activity؛ Zinc؛ Nanoparticle؛ Nanobiotechnology؛ Green Synthesis
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