Modulating effects of melatonin, vitamin C and saffron alone or in combination on radiation induced cell lethality; an in vitro study with Hela cells | ||
| Iranian Journal of Medical Physics | ||
| مقاله 243، دوره 15، Special Issue-12th. Iranian Congress of Medical Physics، بهمن و اسفند 2018، صفحه 244-244 | ||
| نوع مقاله: Conference Proceedings | ||
| شناسه دیجیتال (DOI): 10.22038/ijmp.2018.12874 | ||
| نویسندگان | ||
| Hossein Mozdarani1؛ Sara Ghorbanian2؛ Sohail Mozdarani3؛ Hassan Nosrati4؛ Sahar Mozdarani3 | ||
| 1Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran | ||
| 2Department of Genetics, University of Sistan and Baluchestan, Zahedan, Iran. | ||
| 3Cytogenome Medical Genetic laboratory, Ale-Ahmad Highway, Parvaneh Street, Chamran Medical Building, Tehran, Iran. | ||
| 4Cancer Research Center, Cancer Institute of Iran, Tehran, Iran. | ||
| چکیده | ||
| Introduction: Radiation countermeasures are defined as use of an agent to minimize the deleterious effects of radiation therapy by administering the compound after the radiation exposure has occurred, regardless of toxicity. In spite of extensive study in this field, yet there is no suitable radioprotector introduced. In this study two naturally occurring antioxidants were studied on radiation induced cell lethality. Materials and Methods: Various doses of melatonin, saffron and vitamin C were examined for their antioxidant capacity by using DPPH test. Hela cells were treated with optimum doses of melatonin, saffron and vitamin C; 2 hours prior to gamma irradiation alone or in combination. Treated cells were irradiated with various doses of gamma rays (2, 4, 8 Gy), then cells were plated in 96 well plates for MTT assay. Cells were fixed at 48 and 72 hours after plating and reading was done ELISA reader. Results: Results showed cell viability reduced slightly after 48 h irradiation. Pretreatment of cells with melatonin and vitamin C increased cell lethality considerably for dopses of 2 and 4 Gy, but surprisingly presence of melatonine and vitamin C led to an increase in cell viability when combined with 8 Gy gamma rays. Combination of these agents with saffron showed similar effects. Similar results were observed for 72 h sampling time. However, protective effect of these agents was more pronounced when using 8 Gy radiation dose. Conclusion: Results indicate that despite the antioxidant potential of melatonin, saffron and vitamin C, when combined with low and moderate doses of gamma rays (2 and 4 Gy) led to increased cytotoxic effects of ionizing radiation. However, at high dose of gamma rays (8 Gy) radioprotective effect was seen. The dual action of these agents with low and high doses of ionizing radiation is not known. | ||
| کلیدواژهها | ||
| Radioprotection؛ Natural antioxidant؛ Ionizing radiation؛ Hela cells؛ DPPH assay؛ MTT assay | ||
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