Effect of crocin on biochemical parameters, oxidative/antioxidative profiles, sperm characteristics and testicular histopathology in streptozotocin-induced diabetic rats | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 5، دوره 9، شماره 4، مهر و آبان 2019، صفحه 347-361 اصل مقاله (424.55 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2019.12596 | ||
| نویسندگان | ||
| Seyed Mersad Sefidgar1؛ Mahmood Ahmadi-hamedani* 2؛ Ashkan Jebelli Javan3؛ Reza Narenji Sani2؛ Abbas Javaheri vayghan4 | ||
| 1Student Research Committee, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran | ||
| 2Department of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran | ||
| 3Department of Food Hygiene, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran | ||
| 4Department of Pathobiology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran | ||
| چکیده | ||
| Objective: Chronic hyperglycemia and overproduction of reactive oxygen species (ROS) are strong predictors of the development of reproductive complications of diabetes. The present study was conducted to determine the effects of crocin on biochemical parameters, oxidative stress, and sperm characteristics as well as testes histopathology in diabetic rats. Materials and Methods: Twenty-four rats were divided into the four groups as follows: control, untreated diabetic and two crocin (40 and 60 mg/kg/day)-treated diabetic groups. Diabetes was induced by injection of a single dose of streptozotocin (STZ, 60 mg/kg). Administration of crocin (intraperitoneally) was started three days after STZ injection and was continued until the 28th day. At the end of the experiment, rats were anesthetized after weighing. Blood samples and epididymal sperm were subsequently collected to measure biochemical parameters (glucose and lipid profile), total oxidant and antioxidant status (TOS and TAS, respectively), oxidative stress index (OSI), and sperm characteristics (count, motility, and viability); also, testes were dissected out for histopathology examination. Results: Our result indicated that blood glucose, cholesterol, triglyceride, LDL cholesterol levels, as well as TOS, and OSI decreased, but body weight, sperm counts, motility and viability, as well as TAS and HDL levels increased significantly in the crocin-treated diabetic rats (P˂0.05). In testis sections from diabetic rats treated with crocin (40 and 60 mg/kg), seminiferous tubules exhibited normal shape and restoration of testis architecture was observed. Conclusion: Administration of crocin in the present study, ameliorated blood glucose, lipid abnormalities, oxidative stress, sperm characteristics and testis damage in STZ-diabetic rats. | ||
| کلیدواژهها | ||
| Biochemical parameters؛ Crocin؛ Oxidative stress index؛ Sperm characteristics؛ Streptozotocin؛ Testicular histopathology | ||
| مراجع | ||
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