The role of microRNA-30a and downstream snail1 on the growth and metastasis of melanoma tumor | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 11، دوره 22، شماره 5، مرداد 2019، صفحه 534-540 اصل مقاله (611.72 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2019.32317.7745 | ||
| نویسندگان | ||
| Jahangir Noori1؛ Shaghayegh Haghjooy Javanmard* 2؛ Mohammadreza Sharifi3 | ||
| 1Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| 2Applied Physiology Research Center, Isfahan Cardiovascular Research Institute, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| 3Department of Genetics and Molecular Biology- Isfahan University of Medical Sciences, Isfahan, Iran | ||
| چکیده | ||
| Objective(s): Growing evidences have indicated microRNAs as modulators of tumor development and aggression. On the other hand, a phenomenon known as epithelial-mesenchymal transition (EMT) that indicates a transient phase from epithelial-like features to mesenchymal phenotype is a key player in tumor progression. In this study, we aimed to assess the potential impacts of miR-30a-5p as an inhibitor of melanoma progression and metastasis. Materials and Methods: MiR-30a-5p was transfected into B16-F10 melanoma cells. Then, the B16-F10 cells were injected subcutaneously or intravenously (IV) in to C57BL/6 mice. Then, the mice were euthanized and tumor size, tumor weight, snail1 protein expression and nodules in the lungs were evaluated. Results: The migration of cancerous cells was significantly suppressed in vitro following the ectopic presentation of miR-30a-5p into B16-F10 melanoma cells. Furthermore, the metastatic behavior of the neoplastic cells was further suppressed in a xenograft mouse model of melanoma as observed with limited lung infiltration. We also found that transfected miR-30a-5p into melanoma cells could decrease snail1 and N-cadherin expression. Conclusion: MiR-30a-5p may represent an effective therapeutic target for the management of melanoma and other snail-overexpressing neoplasms. | ||
| کلیدواژهها | ||
| Epithelial-mesenchymal transition؛ Melanoma؛ Metastasis؛ miR-30a؛ Neoplasm؛ Snail1 | ||
| مراجع | ||
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