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Jabini, Raheleh, Eghbali, Seyed Ahmad, Ayatolahi, Hosein, Sheikhi, Maryam, Farzanehfar, Mohammadreza. (1398). Analysis of KRAS gene mutation associated with Helicobacter pylori infection in patients with gastric cancer. , 22(5), 529-533. doi: 10.22038/ijbms.2019.32047.7707
Raheleh Jabini; Seyed Ahmad Eghbali; Hosein Ayatolahi; Maryam Sheikhi; Mohammadreza Farzanehfar. "Analysis of KRAS gene mutation associated with Helicobacter pylori infection in patients with gastric cancer". , 22, 5, 1398, 529-533. doi: 10.22038/ijbms.2019.32047.7707
Jabini, Raheleh, Eghbali, Seyed Ahmad, Ayatolahi, Hosein, Sheikhi, Maryam, Farzanehfar, Mohammadreza. (1398). 'Analysis of KRAS gene mutation associated with Helicobacter pylori infection in patients with gastric cancer', , 22(5), pp. 529-533. doi: 10.22038/ijbms.2019.32047.7707
Jabini, Raheleh, Eghbali, Seyed Ahmad, Ayatolahi, Hosein, Sheikhi, Maryam, Farzanehfar, Mohammadreza. Analysis of KRAS gene mutation associated with Helicobacter pylori infection in patients with gastric cancer. , 1398; 22(5): 529-533. doi: 10.22038/ijbms.2019.32047.7707

Analysis of KRAS gene mutation associated with Helicobacter pylori infection in patients with gastric cancer

Iranian Journal of Basic Medical Sciences
مقاله 10، دوره 22، شماره 5، مرداد 2019، صفحه 529-533    اصل مقاله (498.96 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2019.32047.7707
نویسندگان
Raheleh Jabini1؛ Seyed Ahmad Eghbali2؛ Hosein Ayatolahi3؛ Maryam Sheikhi3؛ Mohammadreza Farzanehfar* 4
1Biotechnology Research Center, Pharmaceutical technology institute, Mashhad University of Medical Sciences, Mashhad, Iran
2Gastroenterology and Hepatology Specialist, Mashhad, Iran
3Pathology Department of Hematology and Blood Banking, Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
4Gastroenterology and Hepatology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): KRAS proto-oncogene mutation can be considered a diagnostic factor for treating various malignancies. Helicobacter pylori infection, a risk factor for stomach cancer, may cause DNA damage and genetic changes. The aim of the current study was to assess the association of gastric cancer and KRAS mutation, demographic factors, and H. pylori infection.
Materials and Methods: DNA was extracted from a total of 140 FFPE gastric cancer tissue samples. detection of KRAS mutation (codons 12 and 13) in tumors was performed by PCR amplification, followed by gel electrophoresis and DNA sequencing. PCR diagnosed any H. pylori infection.
Results: KRAS mutation was detected in 6 of the 140 (4.2%) gastric cancer tissue samples. 18 samples (12.8%), all of which were male (P<0.05), tested positive for H. pylori infection. KRAS mutations were present in 22.2% (4/18) of the samples with H. pylori infection (P<0.05). The mean age of patients was 62.25±12.61 years (range: 30–93 years). A male predominance (2.5 to 1) was reported in the gastric cancers, and at diagnosis, women were significantly younger than men (P=0.004). No association was observed between age or gender and KRAS mutation. Neither was one found between age and H. pylori infection. Tumors from H. pylori+ subjects were significantly more likely to have KRAS mutation than tumors from H. pylori- subjects (OR=17.1).
Conclusion: The data suggest that H. pylori infection when compared with the absence of H. pylori infection, is associated with a higher prevalence of KRAS mutation in gastric cancer.
کلیدواژه‌ها
Helicobacter pylori؛ Gastric cancer؛ KRAS؛ Mutation؛ Sequencing
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