Cytotoxic effects of auraptene against a human malignant glioblastoma cell line | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 4، دوره 9، شماره 4، مهر و آبان 2019، صفحه 334-346 اصل مقاله (788.28 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2019.12384 | ||
| نویسندگان | ||
| Amir R. Afshari1، 2؛ Mostafa Karimi Roshan3؛ Mohammad Soukhtanloo3؛ Ahmad Ghorbani2؛ Farzad Rahmani3؛ Mohammad Jalili-nik3؛ Mohammad Mahdi Vahedi4؛ Azar Hoseini2؛ Hamid R. Sadeghnia1؛ Hamid Mollazadeh5؛ Seyed Hadi Mousavi* 6 | ||
| 1Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 2Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 3Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 4Health Promotion Research Center, Zahedan University of Medical Sciences, Zahedan, Iran | ||
| 5Department of Physiology and Pharmacology, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran | ||
| 6Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| چکیده | ||
| Objective: Glioblastoma multiforme (GBM) is the deadliest type of primary brain tumors, and the survival of patients is estimated to be only about one year. This study, for the first time, investigated the cytotoxic effects of auraptene on U87 GBM cell line. Materials and Methods: The cellular toxicity was measured by the MTT assay following 24 and 48-hr treatment with different concentrations of auraptene (0-400μg/ml). Apoptosis was evaluated by sub-G1 peak in cell cycle analysis of propidium-iodide- stained nuclei. Moreover, to determine the Bax, Bcl-2, MCP-1, NF-κB, IL-1β, and p53 genes expression, we used real-time polymerase chain reaction (RT-PCR). Results: The results revealed that auraptene reduced the viability of U87 cells concentration- and time-dependently with IC50 values of 108.9 and 79.17μg/ml obtained for 24 and 48-hr treatments, respectively. Also, sub-G1 population was significantly increased following 24 (p real-time RT-PCR showed an up-regulation in Bax, NF-κB, IL-1β, and p53 but a down-regulation in MCP-1 and Bcl-2 genes expression. Conclusion: This study showed that auraptene triggered apoptosis probably through Bax/Bcl-2 regulation, blocked cell cycle progression and inhibited proliferation in U87 GBM cells. Taken together, auraptene can be utilized as an effective natural medicine against GBM, after complementary studies. | ||
| کلیدواژهها | ||
| Brain tumors؛ Glioblastoma multiforme؛ Auraptene؛ Cytotoxicity؛ Apoptosis | ||
| مراجع | ||
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