Dual frequency ultrasound-enhanced tissue plasminogen activator thrombolysis in an in vitro human clot model | ||
| Iranian Journal of Medical Physics | ||
| مقاله 83، دوره 15، Special Issue-12th. Iranian Congress of Medical Physics، بهمن و اسفند 2018، صفحه 83-83 | ||
| نوع مقاله: Conference Proceedings | ||
| شناسه دیجیتال (DOI): 10.22038/ijmp.2018.12361 | ||
| نویسندگان | ||
| Mosayyeb Mobasheri1؛ Manijhe Mokhtari-Dizaji2؛ Tayebeh Toliyat3؛ Masoud Mehrpour4 | ||
| 1Department of Medical physics, Medical Sciences, Tarbiat Modares University, Tehran, Iran | ||
| 2Department of Medical physics, Faculty member of Medical Sciences, Tarbiat Modares University, P O Box: 14115-331, Tehran, Islamic Republic of Iran Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran | ||
| 3Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran | ||
| 4Department of Neurology, Firoozgar Hospital, Firoozgar Clinical Research Development Center (FCRDC), Iran University of Medical Sciences (IUMS), Tehran, Iran | ||
| چکیده | ||
| Introduction: Stroke causes death and disability in patients throughout the world. At present, the only FDA- approved drug for ischemic stroke is recombinant tissue plasminogen activator (rt- PA). Unfortunately, rtPA can cause intracerebral hemorrhage and must use within limited time window (within 3-4.5 hour after onset of stroke). Ultrasound with rtPA loaded liposomes (rtPA_L) was suggested as adjuvant therapy that reduce the dose of rt-PA and increase its efficiency. Different frequency protocols such as kHz and MHz were used for sonothrombolysis. In this study, we suggested a protocol including both kHz and MHz with rtPA_L The kHz wave could fragment rtPA_Ls and release rtPA, the MHz enhances lytic efficiency of released rt-PA by stable cavitation and its microstreaming. This protocol may reduce bioeffect of ultrasound wave and simultaneously at least maintain rt-PA lytic effect. Materials and Methods: The rt-PA loaded liposomes were prepared by hydrating lipid film with rt-PA and freezing liposomes under air pressure (4 atm). Cylindrical human whole blood clots (1 mm diameter) formed in and around micropipette at 37 ˚C. In combination with rtPA_L ([rtPA]=50 μg/ml) and human plasma, three protocol were used: 1 MHz (1.5 W/cm2_ 30 minutes), 130 kHz ( 0.01 W/cm2_10 s) + 1 MHz (0.5 W/cm2_ 30 minutes) and control. Clots were imaged under microscope and clot lysis were quantified by measuring clot diameters before and after sonication. Results: Statistical analysis of clot diameter varation between two sonication groups (1 MHz (1.5 W/cm2_ 30 minutes), 130 kHz ( 0.01 W/cm2_10 s) + 1 MHz (0.5 W/cm2_ 30 minutes)) do not show significant difference. Conclusion: Not significant difference between two sonication groups indicates that the dual frequency protocol by using less energy and consequently lower bioeffects may have similar effect as single frequency protocol. | ||
| کلیدواژهها | ||
| Ultrasound Sonothrombolysis؛ Tissue plasminogen activator؛ Liposome | ||
|
آمار تعداد مشاهده مقاله: 564 |
||
| تعداد نشریات | 29 |
| تعداد شمارهها | 2,324 |
| تعداد مقالات | 24,864 |
| تعداد مشاهده مقاله | 82,812,706 |
| تعداد دریافت فایل اصل مقاله | 45,865,547 |