Evaluation of the protective and therapeutic effects of Pistacia atlantica gum aqueous extract on cellular and pathological aspects of experimental asthma in Balb/c mice | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 6، دوره 9، شماره 3، مرداد و شهریور 2019، صفحه 248-259 اصل مقاله (649.3 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2019.12310 | ||
| نویسندگان | ||
| Zaynab Shakrami1، 2؛ hadi Esmaeili Gouvarchin ghaleh3؛ bahman mansouri motlagh1؛ Ali Sheikhian* 4؛ Bahman Jalali Kondori5 | ||
| 1Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran | ||
| 2Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran. | ||
| 3Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. | ||
| 4Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences. | ||
| 5Faculty of medicine, Baqiyatallah University of medical sciences, Tehran, Iran | ||
| چکیده | ||
| Objective: The purpose of this study was to investigate the protective and therapeutic effects of aqueous extract of P. atlantica gum on an experimental asthma in BALB/c mice. Materials and Methods: Aqueous extract of dried and milled P. atlantica gum was assemble andevaluate by GC-MS. In order to investigate the effect of P. atlantica gum extract on cellular and pathological aspects of asthma, 60 BALB/c mice were divided into six groups as: negative control, asthmatic group, asthmatic group receiving dexamethasone (1mg/kg; intraperitoneal (IP)) and three asthmatic groups receiving different concentrations of the extract (100, 200 and 400 mg/kg, orally) from the beginning of the study and continued for 84 days. The examined parameters included cell population, IgE antibody production, levels of IL-4, IL-5, TGF-β, INF-γ, IL-10, and IL-17 cytokines, and lung tissue damage. Results: Regardless of the dose, aqueous extract of P. atlantica gum, caused significant decrease in the number of BALF eosinophilic cells and levels of anti-ovalbumin IgE, IL-4, IL-5 and IL-17 cytokine levels, as well as pathologic damage of the lung tissue. In addition, the amount of anti-inflammatory IL-10, TGF-β, and INF-γ Th1 cytokines significantly increased in the extract-treated groups compared to the asthmatic and dexamethasone-treated groups. Moreover, IFN-γ/IL-4 ratio significantly increased in a dose-dependent manner compared to the un-treated asthma group. Conclusion: The aqueous extract of P. atlantica gum can be considered as a potent anti-inflammatory and immunomodulatory compound and may be used as a natural compound for treatment of immune system disorders. | ||
| کلیدواژهها | ||
| Asthma؛ P. atlantica؛ BALB/c mouse | ||
| مراجع | ||
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