Interactive anticancer effect of nanomicellar curcumin and galbanic acid combination therapy with some common chemotherapeutics in colon carcinoma cells | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 5، دوره 9، شماره 3، مرداد و شهریور 2019، صفحه 237-247 اصل مقاله (1.08 M) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2019.12265 | ||
| نویسندگان | ||
| Arash Jafari1؛ Manouchehr Teymouri2؛ Maryam Ebrahimi Nik3؛ Azam Abbasi4؛ Mehrdad Iranshahi4؛ Mohammad Yahya Hanafi-Bojd* 5؛ Mahmoud Reza Jafari* 6، 7 | ||
| 1School of Medicine, Birjand University of Medical Sciences, Birjand, Iran | ||
| 2Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran. | ||
| 3Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 4Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 5Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran | ||
| 6Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran. | ||
| 7Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran. | ||
| چکیده | ||
| Objective: In the current investigation, we aimed to study the combined cytotoxicity of curcumin, as a nanomicellar formulation, and galbanic acid (Gal), dissolved in DMSO against the murine C26 and human Caco-2 colon carcinoma cells. Further, curcumin potential for cisplatin and doxorubicin (Dox) co-therapy was studied. Materials and Methods: The combined cytotoxic effect of these phytochemicals at varying dose ratios were examined using the MTT colorimetric assay. Moreover, the time-dependent toxicity of curcumin, cisplatin, Dox, and pegylated liposomal Dox (Doxil) was determined. The interactive anti-proliferative behavior of these compounds was examined using the CompuSyn software. Results: Nanomicellar curcumin showed considerable cytotoxicity in C26 cells 24 hr post-treatment. Co-treatment of cells with curcumin nanomicelles: Gal had a synergistic effect in C26 (at 10:1 molar ratio), and Caco-2 (at 1:5 molar ratio) cell lines in cell cultures. Nanomicellar curcumin showed strong and mild synergistic inhibitory effects in C26 cells when co-administered with Doxil and cisplatin, respectively. Conclusion: Curcumin nanomicelles and Gal had a synergistic effect in C26 and Caco-2 cell lines. It is speculated that nanomicellar curcumin shows synergistic cancer cell killing if administered 24-hr post-injection of Doxil and cisplatin. | ||
| کلیدواژهها | ||
| Nanomicellar curcumin؛ Galbanic acid؛ Doxil؛ Cisplatin؛ Combination therapy؛ Synergism | ||
| مراجع | ||
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