Effect of IL-2 co-expressed or co-inoculated with immuno-dominant epitopes from VP1 protein of FMD virus on immune responses in BALB/c mice | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 13، دوره 22، شماره 3، خرداد 2019، صفحه 302-309 اصل مقاله (652.42 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2019.31972.7683 | ||
| نویسندگان | ||
| Mohammad Doosti1؛ Mohammadreza Nassiri* 1، 2؛ Khadijeh Nasiri3؛ Mojtaba Tahmoorespur1؛ saeed Zibaee4 | ||
| 1Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| 2Recombinant Proteins Research Group, Research Institute of Biotechnology and genetic group, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| 3Department of Exercise Physiology, Faculty of Sport Science, University of Mazandaran, Babolsar, Iran 4 Razi Vaccine and Serum Research Institute, Mashhad, Iran | ||
| 4Razi Vaccine and Serum Research Institute, Mashhad, Iran | ||
| چکیده | ||
| Objective(s): The results of studies on vaccine development for foot-and-mouth disease (FMD) virus show that the use of inactivated vaccines for FMD virus is not completely effective. Novel vaccinations based on immuno-dominant epitopes have been shown to induce immune responses. Furthermore, for safety of immunization, access to efficient adjuvants against FMD virus seems to be critical. Materials and Methods: In this study, we produced epitope recombinant vaccines from the VP1 protein of the FMD virus for serotype O of Iran. Constructs were included polytope (tandem-repeat multiple-epitope), polytope coupled with interleukin-2 (polytope-IL 2) as a molecular adjuvant and IL-2. Three expression vectors were constructed and expressed in Escherichia coli BL21 (DE3). To evaluate whether these recombinant vaccines induce immune responses, BALB/c mice were injected with the recombinant vaccines and their immune responses were compared with a negative control group. The humoral and cellular immune responses were measured by ELISA. Results: The results showed that IL-2 co-expressed or co-inoculated with Polytope protein enhances the immune effect of multiple epitope recombinant vaccine against FMD virus. The results of total immunoglobulin G (IgG), IgG1, and IgG2a levels and secretion of interferon gamma (IFN-γ), IL-4 and IL-10 revealed that there were significant differences between negative control group and other injected mice with the recombinant vaccines (P<0.05). Conclusion: Observations indicated that the epitope recombinant plasmid of the VP1 protein co-expressed or co-inoculated with IL-2 was effective in inducing an enhanced immune response. Therefore, IL-2 can be recommended as a potential adjuvant for epitope recombinant vaccine of the VP1 protein from FMD virus. | ||
| کلیدواژهها | ||
| Adjuvant؛ Foot-and-mouth disease-virus؛ Immune response Interleukin-2؛ VP1 protein | ||
| مراجع | ||
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