Protective effects of curcumin on diabetic nephropathy via attenuation of kidney injury molecule 1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) expression and alleviation of oxidative stress in rats with type 1 diabetes | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 6، دوره 22، شماره 4، تیر 2019، صفحه 376-383 اصل مقاله (540.58 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2019.31922.7674 | ||
| نویسندگان | ||
| Hassan Ghasemi1، 2؛ Behzad Einollahi* 2؛ Nejat khiripour3؛ Seyed-Reza Hosseini-Zijoud4؛ Masumeh Farhadiannezhad5 | ||
| 1Department of Clinical Biochemistry, Abadan School of Medical Sciences, Abadan, Iran | ||
| 2Nephrology and Urology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran | ||
| 3Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran | ||
| 4Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran | ||
| 5Student Research Committee, Abadan School of Medical Sciences, Abadan, Iran | ||
| چکیده | ||
| Objective(s): One of the serious complications of Type1 diabetes (T1D) is diabetic nephropathy, which is accompanied with overexpression of kidney injury molecule 1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) and enhanced oxidative stress. The present study was conducted to examine the protective effect of curcumin on the expression of KIM-1, NGAL genes and oxidative damage in the kidney of T1D rats. Materials and Methods: Thirty-six adult male rats were divided into 6 groups (n=6). The control and T1D groups received treatment with curcumin or without it (80 and 130 mg/kg, respectively). After 60 days of treatment, using spectrophotometric methods, biochemical factors and oxidative stress markers were measured. Gene expression of KIM-1 and NGAL was evaluated using quantitative PCR. Also, plasma and urine levels of these two proteins were assayed by the ELISA kit. Results: Diabetes caused a significant increase in the levels of creatinine, FBS, uric acid, urea, and creatinine in the serum, which were attenuated after the administration of curcumin. There was a significant reduction in the values of creatinine, uric acid, and urea in urine in the diabetic group whereas in the rats treated with curcumin, these values were normalized to the normal level (especially in 130 mg/kg). Curcumin administration had a significant role in modulation of serum lipid profile, and it was shown to decrease the kidney and urinary expression levels of KIM-1 and NGAL genes and improve oxidative toxic stress in the kidney tissues. Conclusion: Curcumin can play a protective role in reducing the unpleasant consequences of diabetic nephropathy. | ||
| کلیدواژهها | ||
| Curcumin؛ Kidney injury molecule 1؛ Neutrophil gelatinase associated lipocalin protein؛ Oxidative stress؛ Type 1 diabetes mellitus | ||
| مراجع | ||
|
1. Zarrinkalam E, Ranjbar K, Salehi I, Kheiripour N, Komaki A. Resistance training and hawthorn extract ameliorate cognitive deficits in streptozotocin-induced diabetic rats. Biomed Pharmacother 2018; 97:503-510. 2. Ogurtsova K, da Rocha Fernandes J, Huang Y, Linnenkamp U, Guariguata L, Cho N, et al. IDF Diabetes Atlas: Global estimates for the prevalence of diabetes for 2015 and 2040. Diabetes Res Clin Pract 2017; 128:40-50. 3. Noble JA, Erlich HA. Genetics of type 1 diabetes. Cold Spring Harb Perspect Med 2012; 2:a007732. 4. Steck AK, Rewers MJ. Genetics of type 1 diabetes. Clinical chemistry 2011; 57:176-185. 5. Ziyadeh FN. Mediators of diabetic renal disease: the case for TGF-β as the major mediator. Clin J Am Soc Nephrol 2004; 15:55-57. 6. Ceriello A, Testa R, Genovese S. Clinical implications of oxidative stress and potential role of natural antioxidants in diabetic vascular complications. Nutr Metab Cardiovasc Dis 2016; 26:285-292. 7. Mahmoodnia L, Aghadavod E, Beigrezaei S, Rafieian-Kopaei M. An update on diabetic kidney disease, oxidative stress and antioxidant agents. J Renal Inj Prev 2017; 6: 153-157. 8. Sun L, Dutta RK, Xie P, Kanwar YS. Myo-inositol oxygenase overexpression accentuates generation of reactive oxygen species and exacerbates cellular injury following high glucose ambience a new mechanism relevant to the pathogenesis of diabetic nephropathy. J Biol Chem 2016; 291:5688-5707. 9. Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature 2001; 414: 813-820. 10. Ichimura T, Bonventre JV, Bailly V, Wei H, Hession CA, Cate RL, et al. Kidney injury molecule-1 (KIM-1), a putative epithelial cell adhesion molecule containing a novel immunoglobulin domain, is up-regulated in renal cells after injury. J Biol Chem 1998; 273:4135-4142. 11. Goli F, Karimi J, Khodadadi I, Tayebinia H, Kheiripour N, Hashemnia M, et al. Silymarin attenuates ELMO-1 and KIM-1 expression and oxidative stress in the kidney of rats with type 2 diabetes. Ind J Clin Biochem 2018:1-8. 12. Bonventre JV. Kidney injury molecule-1: a translational journey. Trans Am Clin Climatol Assoc 2014; 125:293-299. 13. Flower DR. Experimentally determined lipocalin structures. Biochim Biophys Acta 2000; 1482:46-56. 14. Gala-Błądzińska A, Dumnicka P, Kuśnierz-Cabala B, Rybak K, Drożdż R, Żyłka A, et al. Urinary neutrophil gelatinase-associated lipocalin is complementary to albuminuria in diagnosis of early-stage diabetic kidney disease in type 2 diabetes. BioMed research international 2017; 2017:4691389. 15. Mishra J, Ma Q, Prada A, Mitsnefes M, Zahedi K, Yang J, et al. Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury. J Am Soc Nephrol 2003; 14:2534-2543. 16. Chou K-M, Lee C-C, Chen C-H, Sun C-Y. Clinical value of NGAL, L-FABP and albuminuria in predicting GFR decline in type 2 diabetes mellitus patients. PLoS One 2013; 8:e54863. 17. Nielsen SE, Andersen S, Zdunek D, Hess G, Parving H-H, Rossing P. Tubular markers do not predict the decline in glomerular filtration rate in type 1 diabetic patients with overt nephropathy. Kidney Int 2011; 79:1113-1118. 18. Blaslov K. Curcumin-a polyphenol with molecular targets for diabetes control? Endocr Oncol Metab 2017; 3: 43-48. 19. Toda S, Miyase T, Arichi H, Tanizawa H, Takino Y. Natural antioxidants. III. Antioxidative components isolated from rhizome of Curcuma longa L. Chem Pharm Bull 1985; 33:1725-1728. 20. Rahimi R, Karimi J, Khodadadi I, Tayebinia H, Kheiripour N, Hashemnia M, et al. Silymarin ameliorates expression of urotensin II (U-II) and its receptor (UTR) and attenuates toxic oxidative stress in the heart of rats with type 2 diabetes. Biomed Pharmacother 2018; 101:244-250. 21. Fleming TH, Theilen T-M, Masania J, Wunderle M, Karimi J, Vittas S, et al. Aging-dependent reduction in glyoxalase 1 delays wound healing. Gerontology 2013; 59:427-437. 22. Kalousova M, Skrha J, Zima T. Advanced glycation end-products and advanced oxidation protein products in patients with diabetes mellitus. Physiol Res 2002; 51:597-604. 23. Moore K, Roberts LJ. Measurement of lipid peroxidation. Free Radical Res 1998; 28:659-671. 24. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 1996; 239:70-76. 25. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005; 38:1103-1111. 26. Hu M, Dillard C. Plasma SH and GSH measurement. Methods Enzymol 1994; 233: 380-385. 27. Pfaffl MW. A new mathematical model for relative quantification in real-time RT–PCR. Nucleic Acids Res 2001; 29:1999-2007. 28. Gupta SC, Patchva S, Koh W, Aggarwal BB. Discovery of curcumin, a component of golden spice, and its miraculous biological activities. Clin Exp Pharmacol Physiol 2012; 39:283-299. 29. Kota N, Panpatil VV, Kaleb R, Varanasi B, Polasa K. Dose-dependent effect in the inhibition of oxidative stress and anticlastogenic potential of ginger in STZ induced diabetic rats. Food Chem 2012; 135:2954-2959. 30. Soetikno V, Sari FR, Sukumaran V, Lakshmanan AP, Mito S, Harima M, et al. Curcumin prevents diabetic cardiomyopathy in streptozotocin-induced diabetic rats: possible involvement of PKC–MAPK signaling pathway. Eur J Pharm Sci 2012; 47:604-614. 31. Hussein MA, El-Maksoud HA. Biochemical effects of resveratrol and curcumin combination on obese diabetic rats. Molecular & Clinical Pharmacology 2013; 4:1-10. 32. Tang Y, Zheng S, Chen A. Curcumin eliminates leptin’s effects on hepatic stellate cell activation via interrupting leptin signaling. Endocrinology 2009; 150:3011-3020. 33. Na L-X, Zhang Y-L, Li Y, Liu L-Y, Li R, Kong T, et al. Curcumin improves insulin resistance in skeletal muscle of rats. Nutr Metab Cardiovasc Dis 2011; 21:526-533. 34. Best L, Elliott AC, Brown PD. Curcumin induces electrical activity in rat pancreatic β-cells by activating the volume-regulated anion channel. Biochem Pharmacol 2007; 73:1768-1775. 35. Pari L, Murugan P. Antihyperlipidemic effect of curcumin and tetrahydrocurcumin in experimental type 2 diabetic rats. Ren Fail 2007; 29:881-889. 36. Ramudu SK, Korivi M, Kesireddy N, Lee L-C, Cheng I-S, Kuo C-H, et al. Nephro-protective effects of a ginger extract on cytosolic and mitochondrial enzymes against streptozotocin (STZ)-induced diabetic complications in rats. Chin J Physiol 2011; 54:79-86. 37. Manley S, Stratton I, Cull C, Frighi V, Eeley E, Matthews D, et al. Effects of three months’ diet after diagnosis of Type 2 diabetes on plasma lipids and lipoproteins (UKPDS 45). Diabet Med 2000; 17:518-523. 38. Sacks FM, Tonkin AM, Craven T, Pfeffer MA, Shepherd J, Keech A, et al. Coronary heart disease in patients with low LDL-cholesterol: benefit of pravastatin in diabetics and enhanced role for HDL-cholesterol and triglycerides as risk factors. Circulation 2002; 105:1424-1428. 39. Hussein H, Abu-Zinadah O. Antioxidant effect of curcumin extracts in induced diabetic wister rats. Int J Zool Res 2010; 6:266-276. 40. Asai A, Miyazawa T. Dietary curcuminoids prevent high-fat diet–induced lipid accumulation in rat liver and epididymal adipose tissue. J Nutr 2001; 131:2932-2935. 41. Alappat L, Awad AB. Curcumin and obesity: evidence and mechanisms. Nutr Rev 2010; 68:729-738. 42. Morakinyo A, Akindele A, Ahmed Z. Modulation of antioxidant enzymes and inflammatory cytokines: possible mechanism of anti-diabetic effect of ginger extracts. Afr J Biomed Res 2011; 14:195-202. 43. Arun N, Nalini N. Efficacy of turmeric on blood sugar and polyol pathway in diabetic albino rats. Plant Foods Hum Nutr 2002; 57:41-52. 44. Nielsen S, Schjoedt K, Astrup A, Tarnow L, Lajer M, Hansen P, et al. Neutrophil gelatinase‐associated lipocalin (NGAL) and kidney injury molecule 1 (KIM1) in patients with diabetic nephropathy: a cross‐sectional study and the effects of lisinopril. Diabet Med 2010; 27:1144-1150. 45. Mishra J, Mori K, Ma Q, Kelly C, Yang J, Mitsnefes M, et al. Amelioration of ischemic acute renal injury by neutrophil gelatinase-associated lipocalin. J Am Soc Nephrol 2004; 15:3073-3082. 46. Alter ML, Kretschmer A, Von Websky K, Tsuprykov G, Reichetzeder C, Simon A, et al. Early urinary and plasma biomarkers for experimental diabetic nephropathy. Clin lab 2012; 58:659-671. 47. Paolisso G, D’Amore A, Galzerano D, Balbi V, Giugliano D, Varricchio M, et al. Daily vitamin E supplements improve metabolic control but not insulin secretion in elderly type II diabetic patients. Diabetes Care 1993; 16:1433-1437. 48. Wu J, Pan X, Fu H, Zheng Y, Dai Y, Yin Y, et al. Effect of curcumin on glycerol-induced acute kidney injury in rats. Sci Rep 2017; 7:10114- 10125. 49. He L, Peng X, Zhu J, Liu G, Chen X, Tang C, et al. Protective effects of curcumin on acute gentamicin-induced nephrotoxicity in rats. Can J Physiol Pharmacol 2015; 93:275-282. 50. Sadar S, Kaspate D, Vyawahare N. Protective effect of L-glutamine against diabetes-induced nephropathy in experimental animal: role of KIM-1, NGAL, TGF-β1, and collagen-1. Ren fail 2016; 38:1483-1495. | ||
|
آمار تعداد مشاهده مقاله: 1,551 تعداد دریافت فایل اصل مقاله: 1,011 |
||