سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Mo, Fang-Fang, Liu, Hai-Xia, Zhang, Yi, Hua, Jing, Zhao, Dan-Dan, An, Tian, Zhang, Dong-Wei, Tian, Tian, Gao, Sihua. (1397). Anti-diabetic effect of loganin by inhibiting FOXO1 nuclear translocation via PI3K/Akt signaling pathway in INS-1 cell. , 22(3), 262-266. doi: 10.22038/ijbms.2019.30246.7294
Fang-Fang Mo; Hai-Xia Liu; Yi Zhang; Jing Hua; Dan-Dan Zhao; Tian An; Dong-Wei Zhang; Tian Tian; Sihua Gao. "Anti-diabetic effect of loganin by inhibiting FOXO1 nuclear translocation via PI3K/Akt signaling pathway in INS-1 cell". , 22, 3, 1397, 262-266. doi: 10.22038/ijbms.2019.30246.7294
Mo, Fang-Fang, Liu, Hai-Xia, Zhang, Yi, Hua, Jing, Zhao, Dan-Dan, An, Tian, Zhang, Dong-Wei, Tian, Tian, Gao, Sihua. (1397). 'Anti-diabetic effect of loganin by inhibiting FOXO1 nuclear translocation via PI3K/Akt signaling pathway in INS-1 cell', , 22(3), pp. 262-266. doi: 10.22038/ijbms.2019.30246.7294
Mo, Fang-Fang, Liu, Hai-Xia, Zhang, Yi, Hua, Jing, Zhao, Dan-Dan, An, Tian, Zhang, Dong-Wei, Tian, Tian, Gao, Sihua. Anti-diabetic effect of loganin by inhibiting FOXO1 nuclear translocation via PI3K/Akt signaling pathway in INS-1 cell. , 1397; 22(3): 262-266. doi: 10.22038/ijbms.2019.30246.7294

Anti-diabetic effect of loganin by inhibiting FOXO1 nuclear translocation via PI3K/Akt signaling pathway in INS-1 cell

Iranian Journal of Basic Medical Sciences
مقاله 7، دوره 22، شماره 3، خرداد 2019، صفحه 262-266    اصل مقاله (559.61 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2019.30246.7294
نویسندگان
Fang-Fang Mo1؛ Hai-Xia Liu1؛ Yi Zhang2؛ Jing Hua3؛ Dan-Dan Zhao1؛ Tian An1؛ Dong-Wei Zhang1؛ Tian Tian1؛ Sihua Gao* 1
1Beijing University of Chinese Medicine, Beijing, 100029, China
2Beijing Open University, Beijing, 100081, China
3Beijing University of Chinese Medicine, Third Affiliated Hospital, Beijing, 100029, China
چکیده
Objective(s): JiangTangXiaoKe (JTXK) granule, a Chinese traditional herbal formula, has been clinically used and demonstrated to be beneficial in controlling high glucose and to relieve the symptoms of  Type 2 diabetes mellitus patients for decades. In this study, we explored how loganin, one of the components in JTXK granule, mediated the anti-diabetic effect.
Materials and Methods: We generate a cell model with the dysfunction of insulin secretion by over-expression FOXO1 in INS-1 cells. MTT method was used to detect cytotoxicity after treated with Loganin. ELISA analysis was used to examine insulin secretion. The expression levels of FOXO1 and Akt were evaluated by Western blot.
Results: Treatment with Loganin did not change the expression level of FOXO1 in INS-1 cells, but increased phosphorylation of FOXO1 and inhibited the nuclear translocation and accumulation of FOXO1, which improved the insulin secretion of the cells. Mechanistically, we found PI3K/Akt signaling pathway involved in these effects, which were blocked by an Akt inhibitor, LY294002.
Conclusion: Loganin mediated the subcellular distribution of FOXO1 via PI3K/Akt signaling pathway, which protected the function of insulin secretion in islet INS-1 cells.
کلیدواژه‌ها
Akt pathway؛ Factor forkhead box O1-(FOXO1)؛ INS-1 cells؛ Loganin؛ Pancreatic β-cell
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