سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Taghizadehghalehjoughi, Ali, Hacimuftuoglu, Ahmet, Cetin, Meltem, ugur, Afifie, butuner, Selcuk, Taspinar, Numan, mohammadzadeh, Maryam. (1397). The effects Metformin/Irinotecan-loaded PLGA nanoparticles on glutamate re-uptake time and alteration EAAT1 gene expression level in vitro. , 6(1), 35-42. doi: 10.22038/nmj.2019.06.005
Ali Taghizadehghalehjoughi; Ahmet Hacimuftuoglu; Meltem Cetin; Afifie busra ugur; Selcuk butuner; Numan Taspinar; Maryam mohammadzadeh. "The effects Metformin/Irinotecan-loaded PLGA nanoparticles on glutamate re-uptake time and alteration EAAT1 gene expression level in vitro". , 6, 1, 1397, 35-42. doi: 10.22038/nmj.2019.06.005
Taghizadehghalehjoughi, Ali, Hacimuftuoglu, Ahmet, Cetin, Meltem, ugur, Afifie, butuner, Selcuk, Taspinar, Numan, mohammadzadeh, Maryam. (1397). 'The effects Metformin/Irinotecan-loaded PLGA nanoparticles on glutamate re-uptake time and alteration EAAT1 gene expression level in vitro', , 6(1), pp. 35-42. doi: 10.22038/nmj.2019.06.005
Taghizadehghalehjoughi, Ali, Hacimuftuoglu, Ahmet, Cetin, Meltem, ugur, Afifie, butuner, Selcuk, Taspinar, Numan, mohammadzadeh, Maryam. The effects Metformin/Irinotecan-loaded PLGA nanoparticles on glutamate re-uptake time and alteration EAAT1 gene expression level in vitro. , 1397; 6(1): 35-42. doi: 10.22038/nmj.2019.06.005

The effects Metformin/Irinotecan-loaded PLGA nanoparticles on glutamate re-uptake time and alteration EAAT1 gene expression level in vitro

Nanomedicine Journal
مقاله 5، دوره 6، شماره 1، فروردین 2019، صفحه 35-42    اصل مقاله (724.67 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22038/nmj.2019.06.005
نویسندگان
Ali Taghizadehghalehjoughi1؛ Ahmet Hacimuftuoglu* 2؛ Meltem Cetin3؛ Afifie busra ugur3؛ Selcuk butuner2؛ Numan Taspinar2؛ Maryam mohammadzadeh2
1Department of Pharmacology and Toxicology, Faculty of Veterinary, Ataturk University, Erzurum, 25240, Turkey
2Department of Medical Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, 25240, Turkey
3Department of Pharmaceutical Technology, Faculty of Pharmacy, Ataturk University, Erzurum, 25240, Turkey
چکیده
Objective(s): The present study was designed to evaluate of Metformin/Irinotecan-loaded poly-lactic-co-glycolic acid (PLGA) nanoparticles (NPs) effects on glutamate re-uptake time and receptor expression status in both glioblastoma multiforme (GBM) and cortex neuron cultures. The study was performed on glioblastoma cell line and primer cortex neuron.
Materials and Methods: The re-uptake time and gene expression status of pure drugs and MET- or IRI-loaded-PLGA NPs on healthy neuron cells and U-87 MG cell line were investigated by using glutamate specific voltammetry electrodes technique and real time PCR.
Results: Both MET and MET-PLGA NPs (1 and 2 mM) exhibited significant cytotoxicity on both U87MG and neuron cells. MET and MET-PLGA NPs (0.5 mM) showed lower cytotoxic effects on both cells. IRI and IRI-PLGA NPs (100 µM) had significant cytotoxic effects on both cell lines.
Conclusion: All drug-loaded NPs caused a significant reduction in glutamate reuptake time compared with free drugs, blank NPs and cancer cells control groups. Consequently, MET- and IRI-loaded PLGA NPs may be a promising approach to treat GBM.
کلیدواژه‌ها
EAAT1؛ Irinotecan؛ Metformin؛ PLGA؛ Voltammetry
مراجع
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