سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Rahimi, Mohsen, Emamgholi, Asgar, Seyyed Tabaei, Seyyed Javad, Khodadoust, Mahdi, Taghipour, Hojat, Jafari, Ameneh. (1397). Perspectives of chitosan nanofiber/film scaffolds with bone marrow stromal cells in tissue engineering and wound dressing. , 6(1), 27-34. doi: 10.22038/nmj.2019.06.004
Mohsen Rahimi; Asgar Emamgholi; Seyyed Javad Seyyed Tabaei; Mahdi Khodadoust; Hojat Taghipour; Ameneh Jafari. "Perspectives of chitosan nanofiber/film scaffolds with bone marrow stromal cells in tissue engineering and wound dressing". , 6, 1, 1397, 27-34. doi: 10.22038/nmj.2019.06.004
Rahimi, Mohsen, Emamgholi, Asgar, Seyyed Tabaei, Seyyed Javad, Khodadoust, Mahdi, Taghipour, Hojat, Jafari, Ameneh. (1397). 'Perspectives of chitosan nanofiber/film scaffolds with bone marrow stromal cells in tissue engineering and wound dressing', , 6(1), pp. 27-34. doi: 10.22038/nmj.2019.06.004
Rahimi, Mohsen, Emamgholi, Asgar, Seyyed Tabaei, Seyyed Javad, Khodadoust, Mahdi, Taghipour, Hojat, Jafari, Ameneh. Perspectives of chitosan nanofiber/film scaffolds with bone marrow stromal cells in tissue engineering and wound dressing. , 1397; 6(1): 27-34. doi: 10.22038/nmj.2019.06.004

Perspectives of chitosan nanofiber/film scaffolds with bone marrow stromal cells in tissue engineering and wound dressing

Nanomedicine Journal
مقاله 4، دوره 6، شماره 1، فروردین 2019، صفحه 27-34    اصل مقاله (640.56 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22038/nmj.2019.06.004
نویسندگان
Mohsen Rahimi1؛ Asgar Emamgholi* 2؛ Seyyed Javad Seyyed Tabaei* 1؛ Mahdi Khodadoust2؛ Hojat Taghipour3؛ Ameneh Jafari4، 5
1Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
3Clinical and Molecular Laboratory, Baqiyatallah Hospital, Tehran, Iran
4Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5Department of Basic Sciences, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
چکیده
Objective (s): Several methods have been proposed for repairing defects and damages, one of which is cell therapy. Bone marrow stromal cells seem to be suitable for this purpose. On the other hand, many biometric materials are used to improve and correct the defects in the body. Nanofibers are widely used in the medical industry, especially in tissue engineering, as scaffolds in wound healing and wound dressing. Chitosan/polyethylene oxide nanofibers can be a suitable replacement for routine wound coverages. Hence, this study was conducted to present a combination of these methods.
Materials and Methods: Chitosan/polyethylene oxide nanofibers and thin films of chitosan were produced and optimized by electron microscopy, on which the bone marrow stromal cells were then cultivated. Interactions between the cells and these biomaterials were investigated through viability, morphology, immunocytochemistry and electron microscopy of cells after 6 days.
All data were analyzed using Student’s t-test and one-way ANOVA tests in SPSS version 16.
PResults: It seems that the high viscosity of chitosan prevents the formation of nanofibers, while chitosan/polyethylene oxide solutions with 80/20 and 90/10 ratios produce perfect, regular, bead free and non-toxic nanofibers with average diameter of 240±10 and 220±10 nm, respectively.
The results of immunocytochemistry and viability showed that the cells had relatively high proliferation on the thin chitosan membranes, while the results of the electron microscopy showed that the morphology of cells was better on the nanofibers than on the thin membrane of chitosan.
Conclusion: Since bone marrow stromal cells were grown well on chitosan-nanofibers, each of them alone was used in the therapeutic methods. It is better to consider a combination of two methods as the treatment method, especially in tissue engineering and cell therapy.
کلیدواژه‌ها
Biocompatible materials؛ Cell- and Tissue-based therapy؛ Nanocomposites؛ Nanostructures؛ Tissue regeneration
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