سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Bahreyni Toossi, Mohammad Taghi, Najafi Amiri, Maryam, Sankian, Mojtaba, Azimian, Hosein, Abdollahi Dehkordi, Sepide, KHADEMI, SARA. (1398). INF/IL-4 increases after the low doses of gamma radiation in BALB/c spleen lymphocytes. , 16(4), 264-269. doi: 10.22038/ijmp.2018.35092.1440
Mohammad Taghi Bahreyni Toossi; Maryam Najafi Amiri; Mojtaba Sankian; Hosein Azimian; Sepide Abdollahi Dehkordi; SARA KHADEMI. "INF/IL-4 increases after the low doses of gamma radiation in BALB/c spleen lymphocytes". , 16, 4, 1398, 264-269. doi: 10.22038/ijmp.2018.35092.1440
Bahreyni Toossi, Mohammad Taghi, Najafi Amiri, Maryam, Sankian, Mojtaba, Azimian, Hosein, Abdollahi Dehkordi, Sepide, KHADEMI, SARA. (1398). 'INF/IL-4 increases after the low doses of gamma radiation in BALB/c spleen lymphocytes', , 16(4), pp. 264-269. doi: 10.22038/ijmp.2018.35092.1440
Bahreyni Toossi, Mohammad Taghi, Najafi Amiri, Maryam, Sankian, Mojtaba, Azimian, Hosein, Abdollahi Dehkordi, Sepide, KHADEMI, SARA. INF/IL-4 increases after the low doses of gamma radiation in BALB/c spleen lymphocytes. , 1398; 16(4): 264-269. doi: 10.22038/ijmp.2018.35092.1440

INF/IL-4 increases after the low doses of gamma radiation in BALB/c spleen lymphocytes

Iranian Journal of Medical Physics
مقاله 2، دوره 16، شماره 4، مهر و آبان 2019، صفحه 264-269    اصل مقاله (1.09 M)
نوع مقاله: Original Paper
شناسه دیجیتال (DOI): 10.22038/ijmp.2018.35092.1440
نویسندگان
Mohammad Taghi Bahreyni Toossi1؛ Maryam Najafi Amiri* 2؛ Mojtaba Sankian3؛ Hosein Azimian1؛ Sepide Abdollahi Dehkordi2؛ SARA KHADEMI4
1Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
2Medical Physics Department,Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
3Immunobiochemistery lab, Bu-Ali (Avecina) Research center, Mashhad University
4Department of Radiology Technology, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Introduction: The effects of the low dose of ionizing radiation are not thoroughly evident due to an unavoidable increase of occupational exposure and the widespread application of ionizing radiation in medical and industrial fields. The aim of this study was to investigate immune system responses following the low doses of ionizing radiation.
Material and Methods: BALB/c mice were exposed to Whole Body Irradiation of 20, 50, and 100 mGy through a 60Co source. Lymphocytes extraction were operated 24 h after irradiation. Afterwards, gene expression analysis was performed with relative quantitative Real-Time polymerase chain reaction to IL-4, IFN-γ, and TGF-β expression levels. Moreover, IFN-γ/IL-4 ratio was computed, and the independent sample t-test was performed for the statistical analysis.
Results: Whereas IL-4, IFN-γ, and TGF-β expression levels decrease after the radiation of the low doses of gamma rays, the IFN-γ/IL-4 ratio increased significantly after irradiation of 20 mGy (P-Value<0.05). However, this ratio did not vary following the gamma irradiation of 50 and 100 mGy.
Conclusion: The positive effects of the low dose of ionizing radiation can be observed through significant alterations in gene expression and the activation of protection mechanisms. This process was caused by the modulation of cellular immune responses after the exposure to 20 mGy. Although the irradiation of higher doses (50 and 100 mGy) induced alterations in the IFN- γ/IL-4 ratio was insignificant. These findings did not confirm the linear no-threshold model theory, and demonstrated that the low dose of ionizing radiation could be the cause of the hormesis phenomenon.
کلیدواژه‌ها
BALB/C Mice؛ Ionizing Radiation؛ IFN-Gamma؛ Interleukin-4؛ TGF-Beta
مراجع
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