Extremely low frequency-pulsed electromagnetic fields affect proangiogenic-related gene expression in retinal pigment epithelial cells | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 3، دوره 22، شماره 2، اردیبهشت 2019، صفحه 128-133 اصل مقاله (537.06 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2018.25023.6214 | ||
| نویسندگان | ||
| Morteza Oladnabi1، 2؛ Abouzar Bagheri3؛ Mozhgan Rezaei Kanavi4؛ Abbas Azadmehr5؛ Anvarsadat Kianmehr* 6، 7 | ||
| 1Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran | ||
| 2Ischemic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran | ||
| 3Department of Clinical Biochemistry and Genetics, Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran | ||
| 4Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran | ||
| 5Immunology Department, Babol University of Medical Sciences, Babol, Iran | ||
| 6Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran | ||
| 7Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran | ||
| چکیده | ||
| Objective(s): It is known that extremely low frequency-pulsed electromagnetic fields (ELF-PEMF) influence multiple cellular and molecular processes. Retinal pigment epithelial (RPE) cells have a significant part in the emergence and pathophysiology of several ocular disorders, such as neovascularization. This study assessed the impact of ELF-PEMF on the proangiogenic features of RPE cells. Materials and Methods: Primary cultured RPE cells were treated with ELF-PEMF (50 Hz) for three days. Using ELISA assay, we evaluated the effects of treatment on RPE cell proliferation and apoptosis. Also, RT-PCR was used to determine the gene expression of proangiogenic factors, such as matrix metalloproteinase-2 (MMP-2), MMP-9, vascular endothelial growth factors receptor 2 (VEGFR-2), hypoxia-inducible factor 1 (HIF-1α), VEGFA, cathepsin D, connective tissue growth factor (CTGF), E2F3, tissue inhibitors of metalloproteinases 1 (TIMP-1), and TIMP-2. Results: No noticeable changes were observed in cell proliferation and cell death of ELF-PEMF-exposed RPE cells, while transcript levels of proangiogenic genes (HIF-1α, VEGFA, VEGFR-2, CTGF, cathepsin D, TIMP-1, E2F3, MMP-2, and MMP-9) increased significantly. Conclusion: RPE cells are important for homeostasis of the retina. ELF-PEMF increased the gene expression of proangiogenic factors in RPE cells, which highlights concerns about the impact of this treatment on human health. | ||
| کلیدواژهها | ||
| ELF-PEMF؛ Gene expression؛ Proangiogenic factors؛ Quantitative real-time PCR؛ RPE cells | ||
| مراجع | ||
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