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Molaie, Soheila, Ghafarifar, Fatemeh, Dalimi, Abdolhossein, Hassan, Zoheir, Sharifi, Zohreh. (1397). Evaluation of synergistic therapeutic effect of shark cartilage extract with artemisinin and glucantime on visceral leishmaniasis in BALB/c mice. , 22(2), 146-153. doi: 10.22038/ijbms.2018.31124.7504
Soheila Molaie; Fatemeh Ghafarifar; Abdolhossein Dalimi; Zoheir Hassan; Zohreh Sharifi. "Evaluation of synergistic therapeutic effect of shark cartilage extract with artemisinin and glucantime on visceral leishmaniasis in BALB/c mice". , 22, 2, 1397, 146-153. doi: 10.22038/ijbms.2018.31124.7504
Molaie, Soheila, Ghafarifar, Fatemeh, Dalimi, Abdolhossein, Hassan, Zoheir, Sharifi, Zohreh. (1397). 'Evaluation of synergistic therapeutic effect of shark cartilage extract with artemisinin and glucantime on visceral leishmaniasis in BALB/c mice', , 22(2), pp. 146-153. doi: 10.22038/ijbms.2018.31124.7504
Molaie, Soheila, Ghafarifar, Fatemeh, Dalimi, Abdolhossein, Hassan, Zoheir, Sharifi, Zohreh. Evaluation of synergistic therapeutic effect of shark cartilage extract with artemisinin and glucantime on visceral leishmaniasis in BALB/c mice. , 1397; 22(2): 146-153. doi: 10.22038/ijbms.2018.31124.7504

Evaluation of synergistic therapeutic effect of shark cartilage extract with artemisinin and glucantime on visceral leishmaniasis in BALB/c mice

Iranian Journal of Basic Medical Sciences
مقاله 6، دوره 22، شماره 2، اردیبهشت 2019، صفحه 146-153    اصل مقاله (632.44 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2018.31124.7504
نویسندگان
Soheila Molaie1، 2؛ Fatemeh Ghafarifar* 1؛ Abdolhossein Dalimi1؛ Zoheir Hassan3؛ Zohreh Sharifi4
1Department of Parasitology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2Deputy of Research, Ardabil University of Medical Sciences, Ardabil, Iran
3Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
4Department of Virology, Iranian Blood Transfusion, Tehran, Iran
چکیده
Objective(s): Because leishmaniasis is related to the impaired functioning of T-cells, the use of an immunomodulator can increase the efficacy of antileishmanial therapy in visceral leishmaniasis. In this study, we used shark cartilage extract with artemisinin and glucantime against visceral leishmaniasis in BALB/c mice, and evaluated the synergistic therapeutic effect.
Materials and Methods: The culturing method and quantitative real-time PCR by using the kDNA gene was used to detect parasite loads in the spleen and liver. INF-γ and IL-4 cytokine levels and survival rates were assayed.
Results: The drug therapy with target drugs reduced parasite burden in the spleen and liver significantly. Although parasite burden was lower in the artemisinin treated group than in the glucantime treated group (P<0.05). The mice survival rate records, throughout the experimental period, showed highly significant survival rates in the test groups compared to the control group (P<0.001). The results of cytokine assay in mice treated with glucantime-shark cartilage extract combination indicated significant increases of IFNγ and IL-4 (P<0.05). Although the increase of IFNγ was more notable than IL-4. The synergistic therapeutic effect is shown in all groups except in the group treated with shark cartilage extract-artemisinin combination. The IFN-γ in glucantime-shark cartilage extract combination treated group was higher than in other groups (P<0.05). The survival rate in this group was more than in other groups too (P<0.05).
Conclusion: Combination therapy with shark cartilage extract as an immunomodulator can increase antileishmanial effects of antimony drugs in VL treatment.
کلیدواژه‌ها
Artemisinin؛ BALB/c mice؛ Glucantim؛ Shark cartilage extract؛ Synergism؛ Visceral leishmaniasis
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