Capsaicin inhibitory effects on Vibrio cholerae toxin genes expression | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 1، دوره 9، شماره 3، مرداد و شهریور 2019، صفحه 187-194 اصل مقاله (466.78 K) | ||
| نوع مقاله: Short communication | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2018.11736 | ||
| نویسندگان | ||
| Soroor Erfanimanesh1؛ gita eslami* 1؛ Arezou Taherpour2؛ Ali Hashemi3 | ||
| 1Infectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran | ||
| 2Department of Microbiology, Kurdistan University of Medical Sciences, Sanandaj, Iran | ||
| 3Depratment of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran | ||
| چکیده | ||
| Objective: Cholera is an acute secretory diarrhea caused by the Gram-negative bacterium, Vibrio cholerae mostly through production of cholera toxin (CT) and zonula occludens toxin (Zot). Isolates of V. cholerae have acquired resistance elements during the last decade. One of the most promising ways to treat resistant strains is to use antivirulence agents instead of killing the causative agent with conventional antibiotics. In this study, we examined whether different concentrations of capsaicin - the pungent fraction of red chili- can act as an antivirulence agent and inhibit V. cholerae toxin production. Materials and Methods: Two standard strains namely, V. cholerae ATCC 14035 and V. cholerae PTCC 1611 were used in this study. Minimum Inhibitory Concentration (MIC) of capsaicin was determined by broth microdilution method. Based on MIC results, the bacteria were cultured in the presence of sub-MIC concentrations of capsaicin and a negative control without capsaicin. Real-time PCR (RT-PCR) was carried out to determine the expression level of V. cholerae toxin genes at each concentration. Results: MIC test showed that 200 mg/mL of capsaicin in 2% dimethyl sulfoxide (DMSO) could inhibit the growth of the two standard strains of V. cholerae. The expression of V. cholerae toxin genes was significantly reduced following treatment with sub-MIC concentrations of capsaicin as assessed by RT-PCR. Conclusion: Capsaicin showed great inhibitory effect against cholera toxin and reduced Zot production in the tested strains of V. cholerae. The results showed promising insights into antivirulence effects of capsaicin. | ||
| کلیدواژهها | ||
| Vibrio cholera؛ Antivirulence agents؛ Capsaicin؛ Toxin gene expression | ||
| مراجع | ||
|
Ahmad B, Rehman MU, Amin I, Arif A, Rasool S, Bhat SA, Afzal I, Hussain I, Bilal S. 2015. A review on pharmacological properties of zingerone (4-(4-Hydroxy-3-methoxyphenyl)-2-butanone). Sci World J, 2015: 816364.
Allen RC, Popat R, Diggle SP, Brown SP. 2014. Targeting virulence: can we make evolution-proof drugs? Nat Rev Microbiol, 12: 300-308.
Anthouard R, DiRita VJ. 2013. Small-molecule inhibitors of toxT expression in Vibrio cholerae. MBio, 4: e00403-13.
Chatterjee S, Asakura M, Chowdhury N, Neogi SB, Sugimoto N, Haldar S, Awasthi SP, Hinenoya A, Aoki S, Yamasaki S. 2010. Capsaicin, a potential inhibitor of cholera toxin production in Vibrio cholerae. FEMS Microbiol Lett, 306: 54-60.
Clatworthy AE, Pierson E, Hung DT. 2007. Targeting virulence: a new paradigm for antimicrobial therapy. Nat Chem Biol, 3:541-548.
Clinical and Laboratory Standards Institute (CLSI): Performance standards for antimicrobial susceptibility testing. 2012. Twenty-second informational supplement. Document M100-S22 Wayne PC.
Fasano A, Fiorentini C, Donelli G, Uzzau S, Kaper JB, Margaretten K, Ding XU, Guandalini S, Comstock L, Goldblum SE. 1995. Zonula occludens toxin modulates tight junctions through protein kinase C-dependent actin reorganization, in vitro. J Clin Invest, 96: 710-720.
Harris JB, LaRocque RC, Qadri F, Ryan ET, Calderwood SB. 2012. Cholera. Lancet (London, England), 30: 2466-2476.
Jensen PG, Curtis PD, Dunn JA, Austic RE, Richmond ME. 2003. Field evaluation of capsaicin as a rodent aversion agent for poultry feed. Pest Manag Sci, 59: 1007-1015.
Kalia NP, Mahajan P, Mehra R, Nargotra A, Sharma JP, Koul S, Khan IA. 2012. Capsaicin, a novel inhibitor of the NorA efflux pump, reduces the intracellular invasion of Staphylococcus aureus. J Antimicrob Chemother, 67: 2401-2408.
Kitaoka M, Miyata ST, Unterweger D, Pukatzki S. 2011. Antibiotic resistance mechanisms of Vibrio cholerae. J Med Microbiol, 60: 397-407.
Lavecchia T, Rea G, Antonacci A, Giardi MT. 2013. Healthy and adverse effects of plant-derived functional metabolites: the need of revealing their content and bioactivity in a complex food matrix. Crit Rev Food Sci Nutr, 53: 198-213.
Laxminarayan R, Duse A, Wattal C, Zaidi AK, Wertheim HF, Sumpradit N, Vlieghe E, Hara GL, Gould IM, Goossens H, Greko C, So AD, Bigdeli M, Tomson G, Woodhouse W, Ombaka E, Peralta AQ, Qamar FN, Mir F, Kariuki S, Bhutta ZA, Coates A, Bergstrom R, Wright GD, Brown ED, Cars O. 2013. Antibiotic resistance-the need for global solutions. Lancet Infect Dis, 13:1057-1098.
Marini E, Magi G, Mingoia M, Pugnaloni A, Facinelli B. 2015. Antimicrobial and anti-virulence activity of capsaicin against erythromycin-resistant, cell-invasive group a streptococci. Front Microbiol, 6:1281-1288.
McMichael C, Barnett J, McMichael AJ. 2012. An ill wind? Climate change, migration, and health. Environ Health Persp, 120: 646-654.
Morkunas B, Gal B, Galloway WR, Hodgkinson JT, Ibbeson BM, Tan YS, Welch M, Spring DR. 2016. Discovery of an inhibitor of the production of the Pseudomonas aeruginosa virulence factor pyocyanin in wild-type cells. Beilstein J Org Chem, 12:1428-1433.
Muanprasat C, Chatsudthipong V. 2013. Cholera: pathophysiology and emerging therapeutic targets. Future med chem, 5: 781-798.
Nataro JP, Kaper JB. Diarrheagenic escherichia coli. 1998. Clin Microbiol Rev, 11:142-201.
Rasko DA, Sperandio V. 2010. Anti-virulence strategies to combat bacteria-mediated disease. Nat Rev Drug Discov, 9: 117-128.
Roca I, Akova M, Baquero F, Carlet J, Cavaleri M, Coenen S, Cohen J, Findlay D, Gyssens I, Heure OE, Kahlmeter G. 2015. The global threat of antimicrobial resistance: science for intervention. New Microbes New Infect, 6: 22-29.
Sack DA. Sack RB, Nair GB, and Siddique AK. 2004. Cholera. Lancet, 363:223-233.
Shakibaie MR, Shahcheraghi F, Hashemi A, Adeli NS. 2008. Detection of TEM, SHV and PER Type Extended-Spectrum ß-Lactamase Genes among Clinical Strains of Pseudomonas aeruginosa Isolated from Burnt Patients at Shafa-Hospital, Kerman, Iran. Iran J Basic MedSci, 11: 104-111.
Silver LL. 2011. Challenges of antibacterial discovery. Clin Microbiol Rev, 24: 71-109.
Srinivasan K. 2016. Biological activities of red pepper (Capsicum annuum) and its pungent principle capsaicin: a review. Crit Rev Food Sci Nutr, 56:1488-1500.
Sumitomo T, Nakata M, Yamaguchi M, Terao Y, Kawabata S. 2012. S-carboxymethylcysteine inhibits adherence of Streptococcus pneumoniae to human alveolar epithelial cells. J Med Microbiol, 61: 101-108.
Trærup SL, Ortiz RA, Markandya A. 2011. The costs of climate change: a study of cholera in Tanzania. Int J Environ Res Public Health, 8: 4386-4405.
Zahid MS, Awasthi SP, Asakura M, Chatterjee S, Hinenoya A, Faruque SM, Yamasaki S. 2015. Suppression of virulence of toxigenic Vibrio cholerae by anethole through the cyclic AMP (cAMP)-cAMP receptor protein signaling system. PloS one, 10: e0137529.
Zhang S, Ma X, Zhang L, Sun H, Liu X. 2017. Capsaicin reduces blood glucose by increasing insulin levels and glycogen content better than capsiate in streptozotocin-induced diabetic rats. J Agric Food Chem, 65: 2323-2330.
Zhong Z, Yu X, Zhu J. 2008. Red bayberry extract inhibits growth and virulence gene expression of the human pathogen Vibrio cholerae. J Antimicrob Chemother, 61: 753-754.
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