Effects of citral on oxidative stress and hepatic key enzymes of glucose metabolism in streptozotocin/high-fat-diet induced diabetic dyslipidemic rats | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 9، دوره 22، شماره 1، فروردین 2019، صفحه 49-57 اصل مقاله (788.59 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2018.26889.6574 | ||
| نویسندگان | ||
| Chetna Mishra1، 2؛ Monowar Khalid2؛ Naazmin Fatima3؛ Babita Singh1؛ Dinesh Tripathi4؛ Mohammad Waseem1؛ Abbas Ali Mahdi* 1 | ||
| 1Department of Biochemistry, King George’s Medical University, Lucknow-226003, Uttar Pradesh, India | ||
| 2Department of Environmental Science, Integral University, Lucknow-226021, Uttar Pradesh, India | ||
| 3Department of Biochemistry, King George’s Medical University, Lucknow-226003, Uttar Pradesh, India Lucknow- 226003, Uttar Pradesh, India | ||
| 4Department of Physiology, King George’s Medical University, Lucknow- 226003, Uttar Pradesh, India | ||
| چکیده | ||
| Objective(s): Phytochemicals such as polyphenols, alkaloids, and terpenoids, protect against the development of early stages and complications of diabetes mellitus according to various reports. The aim of this study was to measure the anti-dyslipidemic and anti-diabetic effects of Citral on high-fat-diet (HFD) and streptozotocin (STZ) induced diabetic dyslipidemic rats and to see also its effect on carbohydrate metabolic regulatory enzymes in the liver. Materials and Methods: Rats were kept on a high-fat diet for 2 weeks, then diabetes was induced by a single dose of STZ (35 mg/kg/BW, intraperitoneally), Citral was administered orally at a dose of 45 mg/kg/BW for 28 days to diabetic rats. Blood glucose, plasma insulin, and lipid profile in blood were studied. Antioxidant activities were assayed in the liver, pancreas, and adipose tissues. Carbohydrate metabolic enzymes of the liver were also studied in diabetic dyslipidemic rats. Results: The results of this study confirmed that administration of Citral significantly (P<0.05) decreased the blood glucose level and increased plasma insulin in diabetic rats. Citral also improved oxidative markers along with anti-oxidative enzymes of the liver, adipose tissue, and pancreas in the HFD/STZ group. Citral also regulated the activity of the glucose-metabolic enzymes in the liver. The results of the present study were compared to Glibenclamide, which is a standard oral drug for lowering the blood sugar. Conclusion: Results may show that Citral possesses anti-dyslipidemic activity as well as anti-diabetic activity and also regulates the enzyme activity of glycolytic and gluconeogenic processes in the liver. | ||
| کلیدواژهها | ||
| Carbohydrate metabolism؛ Citral؛ Diabetes؛ Dyslipidemia؛ Enzymes؛ Oxidative stress؛ Streptozotocin | ||
| مراجع | ||
|
International Diabetes Federation Website. [(Accessed on 9 May 2016)]. http://www.Idf.Org/media-events/press-releases/2015/diabetes-atlas-7th-edition. Kaveeshwar SA, Cornwall J. The current state of diabetes mellitus in India. The Australas Med J 2014; 7:45-48. American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes care 2014; 37(Supplement 1):S81-S90. Maritim A, Dene BA, Sanders RA, Watkins JB. Effects of pycnogenol treatment on oxidative stress in streptozotocin‐induced diabetic rats. J Biochem Mol Toxicol 2003; 17:193-199. Ma Q, Guo Y, Sun L, Zhuang Y. Anti-Diabetic Effects of Phenolic Extract from Rambutan Peels (Nephelium lappaceum) in High-Fat Diet and Streptozotocin-Induced Diabetic Mice. Nutrients 2017; 9:801-812. Elberry AA, Harraz FM, Ghareib SA, Gabr SA, Nagy AA, Abdel-Sattar E. Methanolic extract of Marrubium vulgare ameliorates hyperglycemia and dyslipidemia in streptozotocin-induced diabetic rats. Int J Diabetes Mellit 2015; 3:37-44. Tangvarasittichai S. Oxidative stress, insulin resistance, dyslipidemia and type 2 diabetes mellitus. World J Diabetes 2015; 6:456. Fowler MJ. Microvascular and macrovascular complications of diabetes. Clin Diabetes 2011; 29:116-122. Watkins PJ. ABC of diabetes: cardiovascular disease, hypertension, and lipids. BMJ 2003; 326:874-876. Sivitz WI. Lipotoxicity and glucotoxicity in type 2 diabetes: effects on development and progression. Postgrad Med 2001; 109:55-64. Ozougwu JC, Obimba KC, Belonwu CD, Unakalamba CB. The pathogenesis and pathophysiology of type 1 and type 2 diabetes mellitus. J Physiol Pathophysiol 2013; 4(4):46-57. Wilcox G. Insulin and insulin resistance. Clin Biochemist Rev 2005; 26:19-39. Manvitha K, Bidya B. Review on pharmacological activity of Cymbopogon citratus. Intl J Herbal Med 2014; 1: 5-7. Tiwari M, Dwivedi UN, Kakkar P. Suppression of oxidative stress and pro-inflammatory mediators by Cymbopogon citratus D. Stapf extract in lipopolysaccharide stimulated murine alveolar macrophages. Food Chem Toxicol 2010; 48:2913-2919. Liang CP, Wang M, Simon JE, Ho CT. Antioxidant activity of plant extracts on the inhibition of Citral off‐odor formation. Mol Nutr Food Res 2004; 48:308-317. Srinivasan K, Viswanad B, Asrat L, Kaul CL, Ramarao P. Combination of high-fat diet-fed and low-dose streptozotocin-treated rat: a model for type 2 diabetes and pharmacological screening. Pharmacol Res 2005; 52:313-320. Najafian M, Ebrahim-Habibi A, Yaghmaei P, Parivar K, Larijani, B. Citral as a potential antihyperlipidemic medicine in diabetes: a study on streptozotocin-induced diabetic rats. IJDLD 2011; 10: 1- 8. Modak T, Mukhopadhaya A. Effects of Citral, a naturally occurring antiadipogenic molecule, on an energy-intense diet model of obesity. Ind J Pharmacol 2011; 43:300-305. Husain I, Chander R, Saxena JK, Mahdi AA, Mahdi F. Antidyslipidemic effect of Ocimum sanctum leaf extract in streptozotocin induced diabetic rats. Ind J Clin Biochem 2015; 30:72-77. Kapoor R, Kakkar P. Naringenin accords hepatoprotection from streptozotocin induced diabetes in vivo by modulating mitochondrial dysfunction and apoptotic signaling cascade. Toxicol Reports 2014; 1:569-581. Trinder P. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann Clin Biochem 1969; 6:24-27. Grassi J, Pradelles P, inventors; Commissariat al’Energie Atomique, assignee. Compound labelled by the acetyl cholinesterase of electrophorus electricus, its preparation process and its use as a tracer or marker in enzymoimmunological determinations. United States patent US 5,047,330. 1991 Sep 10. Goldstein DE, Parker KM, England JD, England JE, Wiedmeyer HM, Rawlings SS, et al. Clinical application of glycosylated hemoglobin measurements. Diabetes 1982; 31(Supplement 3):70-78. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193:265-275. Okada M, Matsui H, Ito Y, Fujiwara A, Inano K. Low-density lipoprotein cholesterol can be chemically measured: a new superior method. J Lab Clin Med 1998; 132(3):195-201. Mosinger F. Photometric adaptation of Dole’s microdetermination of free fatty acids. J lipid Res 196; 6:157-159. Deeg R, Ziegenhorn J. Kinetic enzymic method for automated determination of total cholesterol in serum. Clin Chem 1983; 29:1798-1802. Baginski ES, Epstein E, Zak B. The measurement of serum total phospholipids. Ann Clin Lab Sci 1972; 2:255-267. Bucolo G, David H. Quantitative determination of serum triglycerides by the use of enzymes. Clin Chem 1973; 19:476-482. Ohkawa H, Ohishi N. Reaction of thiobarbituric acid with linoleic acid hydroperoxide. J Lipid Res 1978; 19:1053-1057. Reznick AZ, Packer L. Oxidative damage to proteins: Spectrophotometric method for carbonyl assay. Methods Enzymol 1994; 233: 357-363. McCord JM, Fridovich I. Superoxide dismutase an enzymic function for erythrocuprein (hemocuprein). J Biol Chemistry. 1969 Nov 25; 244:6049-6055. Aebi H. Catalase in vitro. Methods Enzymol. 1984; 105:121-126. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70:158-169. Hazelton GA, Lang CA. Glutathione contents of tissues in the aging mouse. Biochem J 1980; 188:25-30. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959; 82:70-77. Sharma C, Manjeshwar R, Weinhouse S. Effects of diet and insulin on glucose-adenosine triphosphate phosphotransferases of rat liver. J Biol Chem 1963; 238:3840-3845. Crane RK, Sols A. Animal tissue hexokinases:(Soluble and particulate forms) Hexose+ ATP→ Hexose-6-P+ ADP. Methods Enzymol 1955; 1:277-286. Fiske CH, Subbarow Y. The colorimetric determination of phosphorus. J Biol Chem 1925; 66:375-400. Parks WC, Drake RL. Insulin mediates the stimulation of pyruvate kinase by a dual mechanism. Biochem J 1982; 208:333-337. Decker T, Lohmann-Matthes ML. A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity. J Immunol Methods 1988; 115:61-69. Ramesh B, Pugalendi KV. Antihyperglycemic effect of umbelliferone in streptozotocin-diabetic rats. J Med Food 2006; 9:562-566. Jainandunsing S, Ozcan B, Rietveld T, van Miert JN, Isaacs AJ, Langendonk JG, et al. Failing beta-cell adaptation in SouthAsian families with a high risk of type 2 diabetes. Acta Diabetol 2015; 52:11–19 Berg AH, Combs TP, Du X, Brownlee M, Scherer PE. The adipocyte secreted protein Acrp30 enhances hepatic insulin action. Nat Med 2001; 7:947–953. Sundaram R, Naresh R, Shanthi P, Sachdanandam P. Modulatory effect of green tea extract on hepatic key enzymes of glucose metabolism in streptozotocin and high fat diet induced diabetic rats. Phytomedicine 2013; 20:577-584. Kirana H, Srinivasan BP. Trichosanthes cucumerina Linn. improves glucose tolerance and tissue glycogen in non insulin dependent diabetes mellitus induced rats. Indian J Pharmacol 2008;3:103–106. Raghavan B, Kumari SK. Effect of Terminalia arjuna stem bark on antioxidant status in liver and kidney of alloxan diabetic rats. Ind J Physiol Pharmacol 2006; 50:133. Daisy P, Eliza J, Farook KA. A novel dihydroxy gymnemic triacetate isolated from Gymnema sylvestre possessing normoglycemic and hypolipidemic activity on STZ-induced diabetic rats. J Ethnopharmacol 2009; 126:339-344. Goodarzi MT, Zal F, Malakooti M, Sadeghian MS. Inhibitory activity of flavonoids on the lens aldose reductase of healthy and diabetic rats. Acta Medica Iranica 2006;44:41-45. Parveen K, Khan R, Siddiqui WA. Antidiabetic effects afforded by Terminalia arjuna in high fat-fed and streptozotocin-induced type 2 diabetic rats. Int J Diab Metab 2011; 19:23-33. Yusufoglu HS, Soliman GA, Abdel-Rahman RF, Abdel-Kader MS, Ganaie MA, Bedir E, et al. Antihyperglycemic and antihyperlipidemic effects of Ferula duranii in experimental type 2 diabetic rats. Int J Pharmacol 2015; 11:532-541. Raz I, Eldor R, Cernea S, Shafrir E. Diabetes: insulin resistance and derangements in lipid metabolism. Cure through intervention in fat transport and storage. Diabetes Metab Res Rev 2005; 21:3-14. Arvind K, Pradeepa R, Deepa R, Mohan V. Diabetes and coronary artery disease. J Med Res 2002; 116:121-132. Yang H, Li X. The role of fatty acid metabolism and lipotoxicity in pancreatic β-cell injury: identification of potential therapeutic targets. Acta Pharma Sin B 2012; 2:396-402. Suanarunsawat T, Ayutthaya WD, Thirawarapan S, Poungshompoo S. Anti-oxidative, anti-hyperglycemic and lipid-lowering effects of aqueous extracts of Ocimum sanctum L. leaves in diabetic rats. Food Nutr Sci 2014; 5:801-811. Tangvarasittichai S. Oxidative stress, insulin resistance, dyslipidemia and type 2 diabetes mellitus. World J Diabetes 2015; 6:456. Chevion M, Berenshtein E, Stadtman ER. Human studies related to protein oxidation: protein carbonyl content as a marker of damage. Free Rad Res 2000; 33:S99-108. Kumar G, Banu GS, Murugesan AG. Effect of Helicteres isora bark extracts on heart antioxidant status and lipid per oxidation in streptozotocin diabetic rats. J Appl Biomed 2008; 6: 89-95. Ahmed S, Rahman A, Alam A, Saleem M, Athar M, Sultana S. Evaluation of the efficacy of Lawsonia alba in the alleviation of carbon tetrachloride-induced oxidative stress. J Ethnopharmacol 2000; 69:157-164. Grassmann J. Terpenoids as plant antioxidants. Vitam Horm 2005; 72:505-535. Murray RK, Granner DK, Mayes PA, Rodwell VW, Biochemistry HS. Appleton and Lange. Norwalk, CT 1993; 518. Pavana P, Sethupathy S, Manoharan S. Antihyperglycemic and antilipidperoxidative effects of Tephrosia purpurea seed extract in streptozotocin induced diabetic rats. Ind J Clin Biochem 2007; 22:77-83. Bechmann LP, Hannivoort RA, Gerken G, Hotamisligil GS, Trauner M, Canbay A. The interaction of hepatic lipid and glucose metabolism in liver diseases. J Hepatol 2012; 56:952-964. Rathi SS, Grover JK, Vats V. The effect of Momordica charantia and Mucuna pruriens in experimental diabetes and their effect on key metabolic enzymes involved in carbohydrate metabolism. Phyto Res 2002; 16:236-243. Pari L, Suman S. Efficacy of naringin on hepatic enzymes of carbohydrate metabolism in streptozotocin-nicotinamide induced type 2 diabetic rats. Int J Pharm Biol Arch 2010; 1:280-286. Grover JK, Vats V, Rathi SS. Anti-hyperglycemic effect of Eugenia jambolana and Tinospora cordifolia in experimental diabetes and their effects on key metabolic enzymes involved in carbohydrate metabolism. J Ethnopharmacol 2000; 73:461-470. Mithieux G, Rajas F, Gautier-Stein A. A novel role for glucose 6-phosphatase in the small intestine in the control of glucose homeostasis. J Biol Chem 2004; 279:44231-44234. Ragavan B, Krishnakumari S. Antidiabetic effect of T. arjuna bark extract in alloxan induced diabetic rats. Ind J Clin Biochem 2006; 21:123-128. RajeswaraReddy S, Lavany T, Ganapathi Narasimhulu KS. Effect of pimpinellatirupatiensison oxidative enzymes in STZ-induced diabetic rat kidney. Iran J Pharma Res 2012; 11:277-286. Talaiezadeh A, Shahriari A, Tabandeh MR, Fathizadeh P, Mansouri S. Kinetic characterization of lactate dehydrogenase in normal and malignant human breast tissues. Cancer Cell Int 2015; 15:19-27. Ramachandran B, kandaswamy M, Narayanan V, Subramanian S. Insulin mimetic effects of macrocyclic binuclear oxovanadium complexes on streptozotocin-induced experimental diabetes in rats.Diabetes Obes. Metab 2003; 5:455–461. Giribabu N, Eswar KK, Swapna RS, Muniandy S, Salleh N. Vitis vinifera (Muscat Variety) seed ethanolic extract preserves activity levels of enzymes and histology of the liver in adult male rats with diabetes. Evid Based Complement Alternat Med 2015; Article ID 542026: 8 pages. Visweswara Rao P, Madhavi K, Dhananjaya Naidu M, Gan SH. Rhinacanthus nasutus ameliorates cytosolic and mitochondrial enzyme levels in streptozotocin-induced diabetic rats. Evid Based Complement Alternat Med 2013; Article ID 486047:6 pages. | ||
|
آمار تعداد مشاهده مقاله: 1,545 تعداد دریافت فایل اصل مقاله: 909 |
||