Potential therapeutic effect of pomegranate seed oil on ovarian ischemia/reperfusion injury in rats | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 10، دوره 21، شماره 12، اسفند 2018، صفحه 1262-1268 اصل مقاله (1 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2018.30149.7268 | ||
| نویسندگان | ||
| Muhammed Yayla* 1؛ Damla Cetin1؛ Yasemen Adali2؛ Pinar Aksu Kilicle3؛ Erdem Toktay4 | ||
| 1Department of Pharmacology, Faculty of Medicine, Kafkas University, Kars, Turkey | ||
| 2Department of Pathology, Highlited sentences should be cahanged as Canakkale Onsekiz Mart University Faculty of Medicine, 17100 Canakkale/Turkey | ||
| 3Department of Biology, Canakkale Onsekiz Mart University Faculty of Medicine, 17100 Canakkale/Turkey | ||
| 4Department of Histology and Embryology, Ataturk University, Faculty of Medicine, 25240 Erzurum/Turkey | ||
| چکیده | ||
| Objective(s): The aim of this study is to determine the therapeutic effects of pomegranate seed oil, which is a powerful antioxidant and anti-inflammatory agent, on ovarian-ischemia and reperfusion injury in rats. Materials and Methods: Fifty-six female albino Wistar rats were divided into 7 equal groups. Group 1; Sham Operation, Group 2; Ischemia, Group 3; Ischemia + Reperfusion, Group 4; Ischemia + Pomegranate 0,32 ml / kg (IP), Group 5; Ischemia + Pomegranate 0.64 ml / kg, Group 6; Ischemia + Pomegranate 0,32 ml / kg + reperfusion, Group 7; Ischemia + Pomegranate 0,64 ml / kg + reperfusion. Three hours after ischemia and 3 hours after reperfusion, the study was terminated. Results: While NADPH oxidase activity, MDA and TNF-α levels were significantly increased, SOD activity and GSH levels were reduced in ischemia and I/R groups. Low dose pomegranate seed oil application reduced significantly oxidative stress and NADPH oxidase activity in both ischemic and ischemic/reperfusion groups. At the same time, low-dose pomegranate seed oil extract reduced TNF-α levels and significantly increased antioxidant activity. Conclusion: PSO demonstrated an important therapeutic effect in the treatment of ovarian ischemia and reperfusion injury. | ||
| کلیدواژهها | ||
| Ischemia/Reperfusion؛ Oxidative stress؛ Ovary؛ Pomegranate seed oil؛ Punicaceae؛ Rats | ||
| مراجع | ||
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