سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Eki̇nci̇-Akdemi̇r, Fazile Nur, Yıldırım, Serkan, Mehmet Kandemir, Fatih, Gülçi̇n, İlhami, Küçükler, sefa, Sağlam, Yavuz Selim, Yakan, Selvinaz. (1397). The effects of casticin and myricetin on liver damage induced by methotrexate in rats. , 21(12), 1281-1288. doi: 10.22038/ijbms.2018.29922.7217
Fazile Nur Eki̇nci̇-Akdemi̇r; Serkan Yıldırım; Fatih Mehmet Kandemir; İlhami Gülçi̇n; sefa Küçükler; Yavuz Selim Sağlam; Selvinaz Yakan. "The effects of casticin and myricetin on liver damage induced by methotrexate in rats". , 21, 12, 1397, 1281-1288. doi: 10.22038/ijbms.2018.29922.7217
Eki̇nci̇-Akdemi̇r, Fazile Nur, Yıldırım, Serkan, Mehmet Kandemir, Fatih, Gülçi̇n, İlhami, Küçükler, sefa, Sağlam, Yavuz Selim, Yakan, Selvinaz. (1397). 'The effects of casticin and myricetin on liver damage induced by methotrexate in rats', , 21(12), pp. 1281-1288. doi: 10.22038/ijbms.2018.29922.7217
Eki̇nci̇-Akdemi̇r, Fazile Nur, Yıldırım, Serkan, Mehmet Kandemir, Fatih, Gülçi̇n, İlhami, Küçükler, sefa, Sağlam, Yavuz Selim, Yakan, Selvinaz. The effects of casticin and myricetin on liver damage induced by methotrexate in rats. , 1397; 21(12): 1281-1288. doi: 10.22038/ijbms.2018.29922.7217

The effects of casticin and myricetin on liver damage induced by methotrexate in rats

Iranian Journal of Basic Medical Sciences
مقاله 13، دوره 21، شماره 12، اسفند 2018، صفحه 1281-1288    اصل مقاله (813.78 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2018.29922.7217
نویسندگان
Fazile Nur Eki̇nci̇-Akdemi̇r* 1؛ Serkan Yıldırım2؛ Fatih Mehmet Kandemir3؛ İlhami Gülçi̇n4؛ sefa Küçükler3؛ Yavuz Selim Sağlam2؛ Selvinaz Yakan5
1Department of Nutrition and Dietetics, Health School, Ağrı İbrahim Çeçen University, Ağrı, Turkey
2Department of Pathology, Faculty of Veterinary, Atatürk University, Erzurum, Turkey
3Department of Biochemistry, Faculty of Veterinary, Atatürk University, Erzurum, Turkey
4Department of Chemistry, Faculty of Science, Atatürk University, Erzurum, Turkey
5Department of Animal Health, School of Eleşkirt Celal Oruç, Ağrı İbrahim Çeçen University, Ağrı, Turkey
چکیده
Objective(s): In this study, we evaluated the therapeutic effects of casticin and myricetin on liver  damage induced by methotrexate in rats.
Materials and Methods: Thirty-six male rats were used for the study and divided into 6 groups: control, methotrexate, casticin, myricetin, casticin+methotrexate, and myricetin+methotrexate. It was performed by methotrexate (20 mg/kg single dose, IP) in methotrexate, casticin+methotrexate and myricetin+methotrexate groups. Casticin 200 mg/kg dose was given to casticin and casticin+methotrexate groups. Myricetin 50 mg/kg dose was given to myricetin and myriceytin+methotrexate groups. At the end of the experiment, liver tissues were removed for the purpose of histopathological, biochemical and immunohistochemical assessments.
Results: In our study, we have detected that MDA levels increased and activities of antioxidant enzymes SOD, CAT, and GPX decreased in the methotrexate group compared to the other groups, but the level of MDA decreased and activities of these enzymes increased in casticin+methotrexate and myricetin+methotrexate groups compared to the methotrexate group. In immunohistochemical examinations of control, casticin and myricetin groups in liver tissues no caspase-3 and 8-OHdG expressions were observed. In the MTX group, caspase-3 and 8-OHdG expressions were seen at the severe levels. Caspase-3 and 8-OHdG expressions were mild in hepatocytes in the casticin+methotrexate and myricetin+methotrexate groups. When the liver tissues of the rats in the methotrexate group were examined, severe pathological damage was detected both in the parietal region and in the portal region.
Conclusion: By looking at these results, we can say that casticin and myricetin are effective against liver damage induced by methotrexate.
کلیدواژه‌ها
Antioxidants؛ Casticin؛ Myricetin؛ Oxidative stress؛ Reactive Oxygen Species
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