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Chen, Yili, Guo, Penghao, Huang, Han, Huang, Yongxin, Wu, Zhongwen, Liao, Kang. (1397). Detection of co-harboring OXA-58 and NDM-1carbapenemase producing genes resided on a same plasmid from an Acinetobacter pittii clinical isolate in China. , 22(1), 106-111. doi: 10.22038/ijbms.2018.28934.6994
Yili Chen; Penghao Guo; Han Huang; Yongxin Huang; Zhongwen Wu; Kang Liao. "Detection of co-harboring OXA-58 and NDM-1carbapenemase producing genes resided on a same plasmid from an Acinetobacter pittii clinical isolate in China". , 22, 1, 1397, 106-111. doi: 10.22038/ijbms.2018.28934.6994
Chen, Yili, Guo, Penghao, Huang, Han, Huang, Yongxin, Wu, Zhongwen, Liao, Kang. (1397). 'Detection of co-harboring OXA-58 and NDM-1carbapenemase producing genes resided on a same plasmid from an Acinetobacter pittii clinical isolate in China', , 22(1), pp. 106-111. doi: 10.22038/ijbms.2018.28934.6994
Chen, Yili, Guo, Penghao, Huang, Han, Huang, Yongxin, Wu, Zhongwen, Liao, Kang. Detection of co-harboring OXA-58 and NDM-1carbapenemase producing genes resided on a same plasmid from an Acinetobacter pittii clinical isolate in China. , 1397; 22(1): 106-111. doi: 10.22038/ijbms.2018.28934.6994

Detection of co-harboring OXA-58 and NDM-1carbapenemase producing genes resided on a same plasmid from an Acinetobacter pittii clinical isolate in China

Iranian Journal of Basic Medical Sciences
مقاله 16، دوره 22، شماره 1، فروردین 2019، صفحه 106-111    اصل مقاله (501.4 K)
نوع مقاله: Short Communication
شناسه دیجیتال (DOI): 10.22038/ijbms.2018.28934.6994
نویسندگان
Yili Chen1؛ Penghao Guo1؛ Han Huang1؛ Yongxin Huang2؛ Zhongwen Wu1؛ Kang Liao* 1
1Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, 510080, Guangzhou, Guangdong, China
2Department of Laboratory Medicine, Zhongshan Medical School of Sun Yat-sen University, 510080, Guangzhou, Guangdong, China
چکیده
Objective(s): Acinetobacter pittii has become an emerging opportunistic noscomial pathogen worldwide with multi-drug resistance. In the present study, an A. pittii strain was isolated from bronchoalveolar lavage fluid sample harboring both OXA-58 and NDM-1carbapenemase producing genes. The mechanisms of carbapenem resistance of the A. pittii strain was investigated.
Materials and Methods: Carbapenemase producing genes were examined by PCR and DNA sequencing. S1-PFGE was used to localize carbapenemase encoding genes. Filter mating and electrotransformation were used to investigate the transferability of such carbapenemase encoding genes between different strains. Genetic surroundings of blaOXA-58 and blaNDM-1 genes were detected as well.
Results: The A. pittii strain, carrying both OXA-58 and NDM-1 carbapenemase encoding genes, was resistant to all β-lactam antibiotics, while suscepitible to ciprofloxacin, levofloxacin, tobramycin, cotrimoxazole and tigecycline. Southern blot hybridization for the blaOXA-58 and blaNDM-1 gene indicated that the two genes locate in the same plasmid with molecular weight of 310.1-336.5kb. BlaOXA-58 was located in an ISAba3-blaOXA-58-ISAba3-like structure, and the blaNDM-1 gene cluster was embedded in an ISAba125-aphA6- blaNDM-1-bleMBL-ΔtrpF-dsbC-cutA structure sequentially.
Conclusion: In the present study, it is first reported an A. pittii clincal strain in China, co-harboring OXA-58 and NDM-1 carbapenemase producing genes residing on a same plasmid. In hospital and community settings, it is of great significance and urgence to increase the surveillance of these kinds of organisms.
کلیدواژه‌ها
Acinetobacter pittii؛ Carbapenemase؛ Co-harboring؛ NDM-1؛ OXA-58
مراجع
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