The protective effect of propofol on hydrogen peroxide-induced human esophageal carcinoma via blocking the Wnt/β-catenin signaling pathway | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 15، دوره 21، شماره 12، اسفند 2018، صفحه 1297-1304 اصل مقاله (1.04 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2018.29141.7039 | ||
| نویسندگان | ||
| Jian-Jun Xue1، 2، 3؛ Ling-Yun Zhang3؛ Huai-Jing Hou3؛ Yan Li3؛ Wan-Sheng Liang3؛ Ke-Hu Yang* 1، 2 | ||
| 1Evidence Based Medicine Centre, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China | ||
| 2Key Laboratory of Evidence Based Medicine and Knowledge Translation of Gansu Province, Lanzhou 730000, China | ||
| 3Gansu Provincial Hospital of TCM, Lanzhou 730050, China | ||
| چکیده | ||
| Objective(s): To analyze the potential influences of propofol on the oxidative stress of H2O2-induced human esophageal squamous cell carcinoma (ESCC) Eca109 cell through mediating the Wnt/β-catenin signaling pathway. Materials and Methods: Eca109 cells were classified into 5 groups: Control group, H2O2 group, Propofol + H2O2 group, Dkk1 (Dickkopf-1, Wnt/β-catenin pathway antagonist) + H2O2 group, and Propofol + LiCl (Lithium chloride, Wnt/β-catenin pathway agonist) + H2O2 group. Western blotting was performed to determine the protein expressions, flow cytometry to measure the content of ROS, immunofluorescence staining to detect the oxidative DNA damage, as well as MTT, AnnexinV-FITC/PI, Wound-healing, and Transwell assays to test the biological characteristics of Eca109 cells. Results: H2O2 resulted in the increased nuclear and cytoplasmatic expression of β-catenin, reduced p-GSK3β expression, up-regulated ROS content, and induced oxidative DNA damage in Eca109 cells. Moreover, Eca109 cells treated with H2O2 alone had enhanced cell proliferation and metastasis but decreased cell apoptosis, as compared with those without any treatment; meanwhile, the declined Cyt C, Bax, and cleaved caspase-3, as well as the elevated Bcl-2 were also observed in Eca109 cells in the H2O2 group, which were reversed by Propofol or Dkk1. Moreover, Propofol could inhibit the effect of LiCl on activating the Wnt/β-catenin signaling pathway in H2O2-induced Eca109 cells. Conclusion: Propofol elicits protective effects to inhibit H2O2-induced proliferation and metastasis and promote apoptosis of Eca109 cells via blocking the Wnt/β-catenin pathway, offering a possible therapeutic modality for ESCC. | ||
| کلیدواژهها | ||
| Esophageal neoplasms؛ Hydrogen peroxide؛ Propofol؛ Wnt signaling pathway؛ β-catenin | ||
| مراجع | ||
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