The Antiapoptotic Effect of Curcumin in the Fibroblast of the Cochlea in an Ototoxic Rat Model | ||
| Iranian Journal of Otorhinolaryngology | ||
| مقاله 1، دوره 30، شماره 5 - شماره پیاپی 100، آذر و دی 2018، صفحه 247-253 اصل مقاله (405.32 K) | ||
| نوع مقاله: Original | ||
| شناسه دیجیتال (DOI): 10.22038/ijorl.2018.26701.1871 | ||
| نویسندگان | ||
| Tengku-Siti-Hajar Haryuna* 1؛ Agustinus-Hamonangan-Winston Purba2؛ Farhat Farhat1؛ Widayat Alviandi3 | ||
| 1Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara,, Medan 20155, Indonesia. | ||
| 2Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan 20155, Indonesia. | ||
| 3Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia. | ||
| چکیده | ||
| Introduction: This study aimed to show the potency of curcumin as an antiapoptotic agent that decreases the apoptotic index in the cochlea lateral wall in ototoxic rat models. Materials and Methods: A total of 24 Rattus norvegicus were divided into eight groups: Group 1 (control group), Group 2 (gentamicin (+)), Group 3 (gentamicin + curcumin 20 mg/day), Group 4 (gentamicin + curcumin 40 mg/day), Group 5 (gentamicin + curcumin 20 mg/day for 7 days), Group 6 (gentamicin + curcumin 40 mg/day for 7 days), Group 7 (curcumin 20 mg/day for 3 days + gentamicin), and Group 8 (curcumin 40 mg/day for 3 days + gentamicin). After the division, the rats were terminated in order to measure the apoptotic index using a terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) assay in the fibroblasts of the cochlea lateral walls. The data were analyzed using analysis of variance (ANOVA), and P Results: Administration of gentamicin showed significant differences (P<0.05) in the apoptotic index. Groups undergoing curcumin treatment at a higher dose (200 mg/kg bw) and the prevention groups showed significant differences compared with groups not treated with curcumin Conclusion: This study concluded that the apoptotic index can be decreased by curcumin and has a preventive benefit toward ototoxic rat models. The administration of curcumin depended on the dose and duration. | ||
| کلیدواژهها | ||
| Apoptosis؛ Curcumin؛ Cochlea؛ Gentamicin؛ Prevention؛ Rats | ||
| مراجع | ||
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