سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Haghighi, Mohammad Bagher, Dara, Naghi, Mansour Ghanaie, Roxana, Azimi, Leila, Hosseini, Amirhossein, Tajalli, Saleheh, Hajipour, Mahmoud, Sayyari, Aliakbar, Imanzadeh, Farid, Khatami, Katayoun, Rohani, Pejman, Olang, Beheshteh. (1397). Evaluation of Clarithromycin and Metronidazole Resistance of Helicobacter Pylori Infection in Symptomatic Iranian Children. , 7(2), 8925-8933. doi: 10.22038/ijp.2018.34347.3028
Mohammad Bagher Haghighi; Naghi Dara; Roxana Mansour Ghanaie; Leila Azimi; Amirhossein Hosseini; Saleheh Tajalli; Mahmoud Hajipour; Aliakbar Sayyari; Farid Imanzadeh; Katayoun Khatami; Pejman Rohani; Beheshteh Olang. "Evaluation of Clarithromycin and Metronidazole Resistance of Helicobacter Pylori Infection in Symptomatic Iranian Children". , 7, 2, 1397, 8925-8933. doi: 10.22038/ijp.2018.34347.3028
Haghighi, Mohammad Bagher, Dara, Naghi, Mansour Ghanaie, Roxana, Azimi, Leila, Hosseini, Amirhossein, Tajalli, Saleheh, Hajipour, Mahmoud, Sayyari, Aliakbar, Imanzadeh, Farid, Khatami, Katayoun, Rohani, Pejman, Olang, Beheshteh. (1397). 'Evaluation of Clarithromycin and Metronidazole Resistance of Helicobacter Pylori Infection in Symptomatic Iranian Children', , 7(2), pp. 8925-8933. doi: 10.22038/ijp.2018.34347.3028
Haghighi, Mohammad Bagher, Dara, Naghi, Mansour Ghanaie, Roxana, Azimi, Leila, Hosseini, Amirhossein, Tajalli, Saleheh, Hajipour, Mahmoud, Sayyari, Aliakbar, Imanzadeh, Farid, Khatami, Katayoun, Rohani, Pejman, Olang, Beheshteh. Evaluation of Clarithromycin and Metronidazole Resistance of Helicobacter Pylori Infection in Symptomatic Iranian Children. , 1397; 7(2): 8925-8933. doi: 10.22038/ijp.2018.34347.3028

Evaluation of Clarithromycin and Metronidazole Resistance of Helicobacter Pylori Infection in Symptomatic Iranian Children

Journal of Pediatric Perspectives
مقاله 1، دوره 7، شماره 2 - شماره پیاپی 62، اردیبهشت 2019، صفحه 8925-8933    اصل مقاله (342.34 K)
شناسه دیجیتال (DOI): 10.22038/ijp.2018.34347.3028
نویسندگان
Mohammad Bagher Haghighi1؛ Naghi Dara* 2؛ Roxana Mansour Ghanaie3؛ Leila Azimi3؛ Amirhossein Hosseini2؛ Saleheh Tajalli4؛ Mahmoud Hajipour5؛ Aliakbar Sayyari2؛ Farid Imanzadeh2؛ Katayoun Khatami2؛ Pejman Rohani2؛ Beheshteh Olang2
1Pediatric resident, Mofid Children Hospital, Faculty of medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2Pediatric Gastroenterology, Hepatology and Nutrition Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3Pediatric Infectious Research center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4Neonatal Health Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
5Student Research Office, Department of Epidemiology, School of Public Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
چکیده
Helicobacter Pylori (H. pylori) as a gram-negative bacterium is the most common infection of the gastrointestinal tract, and worldwide it affects the children over three years of age. H. pylori could cause gastrointestinal and extra-intestinal manifestations. Antibiotic resistance can happen primarily and occurs during treatment. We aimed to evaluate the resistance gene of H. pylori obtained from gastric biopsy by polymerase chain reaction (PCR) method in Iranian children over 3 years old.
Materials and Methods
This study was a cross-sectional to evaluate the resistance gene of H. pylori obtained from gastric biopsy by polymerase chain reaction method for metronidazole and clarithromycin in children over three years old referring to the Mofid Children's Medical Center in Tehran, Iran.
Results: Finally, data from seventy-nine samples included (mean age=10.7 years and male gender = 60.8%). Beta Globulin (BG) gene were detectable in 75 (94.93%) specimens of 79 (100%). Seventeen out of 75 specimens showed positive results for molecular detection of H. pylori. The results of RFLP-PCR technique showed that mutation of RdxA gene in seven of 17 (41.1%) for Metronidazole resistance and one case of 17 (5.8%) mutation of 23Y RNA gene that leads to clarithromycin resistance.
Conclusion:
Regarding the results of our study, it is better to check microbial resistance by culture and antibiogram for the antibiotic regimen of the first and second line of H. pylori treatment in children.
کلیدواژه‌ها
Antibiotic resistance؛ Children؛ Helicobacter pylori؛ Infection
آمار
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