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Eftekhari moghadam, Ali Reza, Saki, Ghasem, Hemadi, Masoud, Mazaheri, Zohre, Khodadadi, Ali. (1397). Effect of dehydroepiandrosterone on meiotic spindle structure and oocyte quality in mice. , 21(10), 1020-1025. doi: 10.22038/ijbms.2018.27111.6629
Ali Reza Eftekhari moghadam; Ghasem Saki; Masoud Hemadi; Zohre Mazaheri; Ali Khodadadi. "Effect of dehydroepiandrosterone on meiotic spindle structure and oocyte quality in mice". , 21, 10, 1397, 1020-1025. doi: 10.22038/ijbms.2018.27111.6629
Eftekhari moghadam, Ali Reza, Saki, Ghasem, Hemadi, Masoud, Mazaheri, Zohre, Khodadadi, Ali. (1397). 'Effect of dehydroepiandrosterone on meiotic spindle structure and oocyte quality in mice', , 21(10), pp. 1020-1025. doi: 10.22038/ijbms.2018.27111.6629
Eftekhari moghadam, Ali Reza, Saki, Ghasem, Hemadi, Masoud, Mazaheri, Zohre, Khodadadi, Ali. Effect of dehydroepiandrosterone on meiotic spindle structure and oocyte quality in mice. , 1397; 21(10): 1020-1025. doi: 10.22038/ijbms.2018.27111.6629

Effect of dehydroepiandrosterone on meiotic spindle structure and oocyte quality in mice

Iranian Journal of Basic Medical Sciences
مقاله 6، دوره 21، شماره 10، دی 2018، صفحه 1020-1025    اصل مقاله (506.58 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2018.27111.6629
نویسندگان
Ali Reza Eftekhari moghadam1؛ Ghasem Saki* 2؛ Masoud Hemadi3؛ Zohre Mazaheri4؛ Ali Khodadadi5
1Physiology Research Center, Faculty of Medicine, Ahvaz Jundishapour University of Medical Science, Ahvaz, Iran
2Physiology Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran
3Fertility and Infertility Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4Department of Anatomical Science, Faculty of Medicine, Tarbiat Modarres University, Tehran, Iran
5Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran
چکیده
Objective(s): Dehydroepiandrosterone (DHEA) has been reported to improve pregnancy chances in women with diminished ovarian reserve (DOR) and to reduce miscarriage rates by 50–80%. This study, therefore, assesses effects of DHEA on number of retrieved oocytes and meiotic spindles.
Materials and Methods: A randomized, prospective, controlled study was conducted on eight groups, four groups of young mice and four elderly. All young and old groups received different oral doses (35, 50, 75 mg/kg) of DHEA for 3 months. Meiotic spindle assessment was done by immunocytochemical techniques using a confocal laser microscope (Leica TCS-4D).
Results: Statistical surveys showed that in control young groups 80% (P=0.0845) and in the old control group 73.3% (P=0.000) of the meiotic spindles have a normal shape and structure; the difference was meaningful. The young with 50 mg/kg of DHEA in 85.4% and the young with 75 mg/kg of DHEA in 84.2% were normal in shape and structure. Statistical analysis showed that the difference was meaningless (P=0.845). The old group with 30 mg/kg of DHEA in 81.1%, the old with 50 mg/kg of DHEA in 83.9%, and the old with 75 mg/kg of DHEA in 79.0% showed normal shape and structure. The meiotic spindle disruption ratio in old mice showed a significant difference (P=0.000) in comparison with others in young groups. Statistical analysis showed that difference between DHEA and control groups is meaningful. But this difference was meaningless between DHEA groups.
Conclusion: Results showed that DHEA has a positive and improvement effect on the meiotic spindle in old mice.
کلیدواژه‌ها
DHEA؛ Meiotic spindle؛ Mice؛ Oocyte quality؛ pregnancy
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