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Vadaye kheiry, Elahe, Parivar, kazem, Baharara, Javad, Fazly Bazzaz, Bibi Sedigheh, Iranbakhsh, Alireza. (1397). The osteogenesis of bacterial cellulose scaffold loaded with fisetin. , 21(9), 965-971. doi: 10.22038/ijbms.2018.25465.6296
Elahe Vadaye kheiry; kazem Parivar; Javad Baharara; Bibi Sedigheh Fazly Bazzaz; Alireza Iranbakhsh. "The osteogenesis of bacterial cellulose scaffold loaded with fisetin". , 21, 9, 1397, 965-971. doi: 10.22038/ijbms.2018.25465.6296
Vadaye kheiry, Elahe, Parivar, kazem, Baharara, Javad, Fazly Bazzaz, Bibi Sedigheh, Iranbakhsh, Alireza. (1397). 'The osteogenesis of bacterial cellulose scaffold loaded with fisetin', , 21(9), pp. 965-971. doi: 10.22038/ijbms.2018.25465.6296
Vadaye kheiry, Elahe, Parivar, kazem, Baharara, Javad, Fazly Bazzaz, Bibi Sedigheh, Iranbakhsh, Alireza. The osteogenesis of bacterial cellulose scaffold loaded with fisetin. , 1397; 21(9): 965-971. doi: 10.22038/ijbms.2018.25465.6296

The osteogenesis of bacterial cellulose scaffold loaded with fisetin

Iranian Journal of Basic Medical Sciences
مقاله 14، دوره 21، شماره 9، آذر 2018، صفحه 965-971    اصل مقاله (872.39 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2018.25465.6296
نویسندگان
Elahe Vadaye kheiry1؛ kazem Parivar* 1؛ Javad Baharara2؛ Bibi Sedigheh Fazly Bazzaz3، 4؛ Alireza Iranbakhsh1
1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Research Center for Animal Development and Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
3Biotechnology Research Center, Institute of Pharmaceutical Technology, Mashhad University of Medical Sciences, Mashhad, Iran
4School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Bacterial cellulose (BC) has applications in medical science, it is easily synthesized, economic and purer compared to plant cellulose. The present study aimed to evaluate BC, a biocompatible natural polymer, as a scaffold for the bone marrow mesenchymal stem cells (BMSCs) loaded with fisetin, a phytoestrogen.
Materials and Methods: BC hydrogel scaffold was prepared from Gluconaceter xylinus and characterized through scanning electron microscopy (SEM). Biocompatibility of BC was measured by MTT assay, BMSCs were obtained from femur of rat and the osteogenic potential of the BC scaffold cultured with BMSCs and loaded with fisetin, was investigated by measuring the alkaline phosphatase (ALP) activity, alizarin red staining (ARS) and real-time PCR in terms of osteoblast-specific marker, osteocalcin (OCN) and osteopontin (OPN).
Results: Biocompatibility results did not show any toxic effects of BC scaffold on BMSCs, while it increased cell viability. The data showed that BC loaded fisetin differentiated BMSCs into osteoblasts as demonstrated by ALP activity assays and ARS in vitro. Moreover, results from gene expression assay showed the expression of OCN and OPN genes was increased in cells that were seeded on the BC scaffold loaded with fisetin.
Conclusion: According to the results of the present study, BC loaded with fisetin is an effective strategy to promote osteogenic differentiation and a proper localized delivery system, which could be a potential candidate in bone tissue engineering.
کلیدواژه‌ها
Bacterial cellulose؛ Fisetin؛ Mesenchymal stem cells؛ OCN؛ OPN
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