Immunogenicity evaluation of plasmids encoding Brucella melitensis Omp25 and Omp31 antigens in BALB/c mice | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 13، دوره 21، شماره 9، آذر 2018، صفحه 957-964 اصل مقاله (875.58 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2018.27540.6722 | ||
| نویسندگان | ||
| Moslem Shojaei1؛ Mojtaba Tahmoorespur* 1؛ Mahdi Soltani2؛ Mohammad Hadi Sekhavati1 | ||
| 1Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| 2Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran | ||
| چکیده | ||
| Objective(s): Vaccination is one of the most effective means to protect humans and animals against brucellosis. Live attenuated Brucella vaccines are considered effective in animals but they may be potentially infectious to humans, so it is vital to improve the immunoprotective effects and safety of vaccines against Brucella. This study was designed to evaluate the immunogenicity of DNA vaccines encoding B. melitensis outer membrane proteins (Omp25 and Omp31) against B. melitensis Rev1 in a mouse model. Materials and Methods: For this propose, Omp25 and Omp31 genes were cloned (individually and together) into the eukaryotic expression vector pcDNA3.1/Hygro (+). Expressions of recombinant plasmids were confirmed by SDS-PAGE and Western blot analysis. Six groups of BALB/c mice (seven mice per group) were intramuscularly injected with three recombinant constructs, native pcDNA3.1/Hygro (+) and phosphate-buffered saline (PBS) as controls and subcutaneous injection of attenuated live vaccine Rev1. Results: Results indicated that DNA vaccine immunized BALB/c mice had a dominant immunoglobulin G response and elicited a T-cell-proliferative response and induced significant levels of interferon gamma (INF-γ) compared to the control groups. Conclusion: Collectively, these finding suggested that the pcDNA3.1/Hygro DNA vaccines encoding Omp25 and Omp31 genes and divalent plasmid were able to induce both humoral and cellular immunity, and had the potential to be a vaccine candidate for prevention of B. melitensis infections. | ||
| کلیدواژهها | ||
| Brucella melitensis؛ DNA vaccine؛ Omp25؛ Omp31؛ Protective immunity | ||
| مراجع | ||
|
1. Pappas G, Papadimitriou P, Akritidis N, Christou L, Tsianos E. The new global map of human brucellosis. Lancet Infect Dis 2006; 6: 91–99. 2. Hilleman MR. Vaccines in historic evolution and perspective: a narrative of vaccine discoveries. Vaccine 2000; 18:1436-1447. 3. Yang X, Skyberg JA, Cao L, Clapp B, Thornburg T, Pascual DW. Progress in Brucella vaccine development. Front Biol 2013; 8:60-77. 4. Kutzler MA, Weiner DB. DNA vaccines: ready for prime time? Nature Rev Genet 2008; 9:776-788. 5. Caro-Hernández P, Fernández-Lago L, de Miguel M-J, Martín-Martín AI, Cloeckaert A, Grilló M-J, et al. Role of the Omp25/Omp31 family in outer membrane properties and virulence of Brucella ovis. Infect Immun 2007; 75:4050-4061. 6. Bowden RA, Cloeckaert A, Zygmunt MS, Bernard S, Dubray G. Surface exposure of outer membrane protein and lipopolysaccharide epitopes in Brucella species studied by enzyme-linked immunosorbent assay and flow cytometry. Infect Immun 1995; 63:3945-3952. 7. Zygmunt MS, Debbarh HS-A, Cloeckaert A, Dubray G. Antibody response to Brucella melitensis outer membrane antigens in naturally infected and Rev1 vaccinated sheep. Vet Microbiol 1994; 39:33-46. 8. Cloeckaert A, Verger J-M, Grayon M, Grepinet O. Restriction site polymorphism of the genes encoding the major 25 kDa and 36 kDa outer-membrane proteins of Brucella. Microbiology 1995; 141:2111-2121. 9. Commander NJ, Spencer SA, Wren BW, MacMillan AP. The identification of two protective DNA vaccines from a panel of five plasmid constructs encoding Brucella melitensis 16M genes. Vaccine 2007; 25:43-54. 10. Vahedi F, Talebi A, Ghorbani E, Behroozikhah A, Shahriari Ahmadi F, Mahmoudi M. Isolation, cloning and expression of the Brucella melitensis Omp31 gene. Iran J Vet Res 2011; 12:156-162. 11. Johnston D, Bystryn J-C. Heterogeneous antibody response to polyvalent melanoma vaccines in syngeneic mice. Cancer Immunol Immunother. 2005; 54:345-350. 12. Sambrook J, Fritsch E. Maniatis T. Molecular Cloning A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY; 1989. 13. Zheng W, Wang Y, Zhang Z, Yan F. Immunological characteristics of outer membrane protein omp31 of goat Brucella and its monoclonal antibody. Genet Mol Res 2015; 14:11965-11974. 14. Yang Y, Wang L, Yin J, Wang X, Cheng S, Lang X, et al. Immunoproteomic analysis of Brucella melitensis and identification of a new immunogenic candidate protein for the development of brucellosis subunit vaccine. Mol Immunol 2011; 49:175-184. 15. Barquero-Calvo E, Martirosyan A, Ordoñez-Rueda D, Arce-Gorvel V, Alfaro-Alarcón A, Lepidi H, et al. Neutrophils exert a suppressive effect on Th1 responses to intracellular pathogen Brucella abortus. PLoS Pathog 2013; 9:e1003167. 16. Edmonds MD, Cloeckaert A, Booth NJ, Fulton WT, Hagius SD, Walker JV, et al. Attenuation of a Brucella abortus mutant lacking a major 25 kDa outer membrane protein in cattle. Am J Vet Res 2001; 62:1461-1466. 17. Ghasemi A, Jeddi-Tehrani M, Mautner J, Salari MH, Zarnani A-H. Simultaneous immunization of mice with Omp31 and TF provides protection against Brucella melitensis infection. Vaccine 2015; 33:5532-5538. 18. Denoel P, Vo T-O, Weynants V, Tibor A, Gilson D, Zygmunt M, et al. Identification of the major T-cell antigens present in the Brucella melitensis B115 protein preparation, Brucellergene OCB. J Med Microbiol 1997; 46:801-806. 19. Murphy EA, Sathiyaseelan J, Parent MA, Zou B, Baldwin CL. Interferon‐γ is crucial for surviving a Brucella abortus infection in both resistant C57BL/6 and susceptible BALB/c mice. Immunology 2001; 103:511-518. 20. Vitry M-A, De Trez C, Goriely S, Dumoutier L, Akira S, Ryffel B, et al. Crucial role of gamma interferon-producing CD4+ Th1 cells but dispensable function of CD8+ T cell, B cell, Th2, and Th17 responses in the control of Brucella melitensis infection in mice. Infect Immun 2012; 80:4271-4280. 21. Velikovsky CA, Cassataro J, Giambartolomei GH, Goldbaum FA, Estein S, Bowden RA, et al. A DNA vaccine encoding lumazine synthase from Brucella abortus induces protective immunity in BALB/c mice. Infect Immun 2002; 70:2507-2511. 22. Sislema-Egas F, Céspedes S, Fernández P, Retamal-Díaz A, Sáez D, Oñate A. Evaluation of protective effect of DNA vaccines encoding the BAB1_0263 and BAB1_0278 open reading frames of Brucella abortus in BALB/c mice. Vaccine 2012; 30:7286-7291. 23. Yin D, Li L, Song D, Liu Y, Ju W, Song X, et al. A novel recombinant multi-epitope protein against Brucella melitensis infection. Immunol Lett 2016; 175:1-7. 24. Al-Mariri A, Akel R, Abbady A. A DNA vaccine encoding p39 and sp41 of Brucella melitensis induces protective immunity in BALB/c mice. Arch Med Vet 2014; 46:53-62. 25. Cassataro J, Estein SM, Pasquevich KA, Velikovsky CA, de la Barrera S, Bowden R, et al. Vaccination with the recombinant Brucella outer membrane protein 31 or a derived 27-amino-acid synthetic peptide elicits a CD4+ T helper 1 response that protects against Brucella melitensis infection. Infect Immun 2005; 73:8079-8088. 26. Leclerq S, Harms JS, Rosinha GM, Azevedo V, Oliveira SC. Induction of a Th1-type of immune response but not protective immunity by intramuscular DNA immunisation with Brucella abortus GroEL heat-shock gene. J Med Microbiol 2002; 51:20-26. 27. Luo D, Ni B, Li P, Shi W, Zhang S, Han Y, et al. Protective immunity elicited by a divalent DNA vaccine encoding both the L7/L12 and Omp16 genes of Brucella abortus in BALB/c mice. Infect Immun 2006; 74:2734-2741. 28. Cassataro J, Pasquevich KA, Estein SM, Laplagne DA, Velikovsky CA, de la Barrera S, et al. A recombinant subunit vaccine based on the insertion of 27 amino acids from Omp31 to the N-terminus of BLS induced a similar degree of protection against B. ovis than Rev. 1 vaccination. Vaccine 2007; 25:4437-4446. 29. Ranjbar R, Sharifimoghadam S, Saeidi E, Jonaidi N, Sheikhshahrokh A. Induction of protective immune responses in mice by double DNA vaccine encoding of Brucella melitensis Omp31 and Escherichia coli Eae genes. Trop J Pharma Res 2016; 15:2077-2083. | ||
|
آمار تعداد مشاهده مقاله: 907 تعداد دریافت فایل اصل مقاله: 630 |
||