Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 3، دوره 21، شماره 9، آذر 2018، صفحه 884-888 اصل مقاله (535.52 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2018.26191.6432 | ||
| نویسندگان | ||
| Farideh Ranjibar1؛ Mahdi Habibi-Anbouhi2؛ Fatemeh Kazemi-Lomedasht1؛ Seyed Hamid Aghaee-Bakhtiyari3، 4؛ Ehsan Alirahimi1؛ Mahdi Behdani* 1 | ||
| 1Biotechnology Research Center, Venom & Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, Iran | ||
| 2National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran | ||
| 3Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 4Department of Medical Biotechnology, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| چکیده | ||
| Objective(s): Filamentous bacteriophage M13 was genetically engineered to specifically target mammalian cells for gene delivery purpose. Materials and Methods: A vascular endothelial growth factor receptor 2 (VEGFR2)-specific nanobody was genetically fused to the capsid gene III of M13 bacteriophage (pHEN4/3VGR19). A mammalian expression construct containing Cop-green fluorescent protein (Cop-GFP), as a reporter gene, was amplified by PCR and then sub-cloned in the pHEN4/3VGR19 phagemid. The resulting construct was transfected into 293KDR cell. The recombinant phage was extracted and confirmed and then transduced into VEGFR2 expressing cell (293KDR). Results: Seventy-two hr after transfection, green fluorescence was detected in 30% of the cells. About 1% of the cells which transduced by recombinant phages were able to express GFP. Conclusion: It is hoped that the results from this study will help to find potential vectors to improve the efficiency of gene delivery. Taken together, we conclude that this newly-introduced vector can be used in cancer researches. | ||
| کلیدواژهها | ||
| Bacteriophage؛ Nanobody؛ Receptor-mediated gene transfer؛ Targeted-gene delivery؛ VEGFR2 | ||
| مراجع | ||
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