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Widowati, Wahyu, Laksmitawati, Dian, Wargasetia, Teresa, Afifah, Ervi, Amalia, Annisa, Arinta, Yukko, Rizal, Rizal, Suciati, Tri. (1397). Mangosteen peel extract (Garcinia mangostana L.) as protective agent in glucose-induced mesangial cell as in vitro model of diabetic glomerulosclerosis. , 21(9), 972-977. doi: 10.22038/ijbms.2018.29349.7094
Wahyu Widowati; Dian Laksmitawati; Teresa Wargasetia; Ervi Afifah; Annisa Amalia; Yukko Arinta; Rizal Rizal; Tri Suciati. "Mangosteen peel extract (Garcinia mangostana L.) as protective agent in glucose-induced mesangial cell as in vitro model of diabetic glomerulosclerosis". , 21, 9, 1397, 972-977. doi: 10.22038/ijbms.2018.29349.7094
Widowati, Wahyu, Laksmitawati, Dian, Wargasetia, Teresa, Afifah, Ervi, Amalia, Annisa, Arinta, Yukko, Rizal, Rizal, Suciati, Tri. (1397). 'Mangosteen peel extract (Garcinia mangostana L.) as protective agent in glucose-induced mesangial cell as in vitro model of diabetic glomerulosclerosis', , 21(9), pp. 972-977. doi: 10.22038/ijbms.2018.29349.7094
Widowati, Wahyu, Laksmitawati, Dian, Wargasetia, Teresa, Afifah, Ervi, Amalia, Annisa, Arinta, Yukko, Rizal, Rizal, Suciati, Tri. Mangosteen peel extract (Garcinia mangostana L.) as protective agent in glucose-induced mesangial cell as in vitro model of diabetic glomerulosclerosis. , 1397; 21(9): 972-977. doi: 10.22038/ijbms.2018.29349.7094

Mangosteen peel extract (Garcinia mangostana L.) as protective agent in glucose-induced mesangial cell as in vitro model of diabetic glomerulosclerosis

Iranian Journal of Basic Medical Sciences
مقاله 15، دوره 21، شماره 9، آذر 2018، صفحه 972-977    اصل مقاله (654.66 K)
نوع مقاله: Short Communication
شناسه دیجیتال (DOI): 10.22038/ijbms.2018.29349.7094
نویسندگان
Wahyu Widowati* 1؛ Dian Laksmitawati2؛ Teresa Wargasetia1؛ Ervi Afifah3؛ Annisa Amalia3؛ Yukko Arinta3؛ Rizal Rizal3؛ Tri Suciati4
1Faculty of Medicine, Maranatha Christian University, Bandung 40164, West Java, Indonesia
2Faculty of Pharmacy, Pancasila University, Jakarta Selatan 12630, DKI Jakarta, Indonesia
3Aretha Medika Utama, Biomolecular and Biomedical Research Center, Bandung 40163, West Java, Indonesia
4School of Pharmacy Bandung Insitute of Technology, Bandung 40132, West Java, Indonesia
چکیده
Objective(s): This study aims to evaluate the activity of mangosteen peels extract (MPE) as protection agent on induced-glucose mesangial cells (SV40 MES 13 cell line (Glomerular Mesangial Kidney, Mus Musculus)).  
Materials and Methods: MPE was performed based on maceration method. Cytotoxic assay was performed based on MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) method, while the level of TGF-β1 (Transforming growth factor-β1) and fibronectin in glucose-induced mesangial cells were assayed and determined using ELISA KIT.
Results: In viability assay, MPE 5 and 20 µg/ml has the highest activity to increase cells proliferation in glucose-induced mesangial cells at 5, 10, and 15 days of incubation in glucose concentration (5 and 25 mM) (P<0.05). In inhibitory activity of TGF-β1 and fibronectin level, MPE 5 µg/ml (glucose-induced 5 mM) show the lowest level compared to positive control and other treatments (P<0.05).
Conclusion: MPE can increase cell proliferation in glucose-induced mesangial cells and significantly reduce the level of TGF-β1 and fibronectin. MPE activity has correlates to inhibit the diabetic glomerulosclerosis condition and may increase mesangial cell proliferation.
کلیدواژه‌ها
Fibronectin؛ Glomerulosclerosis؛ Garcinia mangostana؛ Mesangial cell؛ Transforming growth factor-β1
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