Targeted detection of the cancer cells using the anti-CD24 bio modified PEGylated gold nanoparticles: the application of CD24 as a vital cancer biomarker | ||
| Nanomedicine Journal | ||
| مقاله 7، دوره 5، شماره 3، مهر 2018، صفحه 172-179 اصل مقاله (509.51 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22038/nmj.2018.005.0007 | ||
| نویسندگان | ||
| Mona Fazel-Ghaziyani1؛ Daryoush Shahbazi-Gahrouei* 1؛ Mohammad Pourhassan-Moghaddam2، 3؛ Behzad Baradaran3؛ Mostafa Ghavami4 | ||
| 1Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| 2Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 3Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| 4Department of Radiology, Paramedical School, Tabriz University of Medical Sciences, Tabriz, Iran | ||
| چکیده | ||
| Objective(s): The central role of molecular imaging modalities in cancer management is an undeniable fact that could help to diagnose cancer tumors in early stages. The main aim of this study is to prepare a novel targeted molecular imaging nanoprobe of CD24-PEGylated Au NPs to improve the ability of Computed tomography scanning (CT scan) outputs for both in vitro and in vivo detection of breast cancer (4T1) cells. Materials and methods: Gold nanoparticles (Au NPs) were synthesized and coated with polyethylene glycol (PEG) chains in order to improve the stability of the Au NPs and to provide bio modification points for antibody immobilization. The synthesized nanoprobe was used for both in vitro and in vivo targeted CT imaging breast cancer cells (4T1) and the xenograft tumor model. Results: Findings showed that the attenuation coefficient of 4T1 cells that were targeted by CD24-PEGylated Au NPs is calculated to be over two times higher than the untargeted 4T1 cells (15 HU vs 39 HU, respectively). Indeed, the results clearly reveal that the developed CD24-PEGylated Au NPs showed the tumor CT enhancement was higher than that of Omnipaqe which used as control. Also, the CT number values of the tumor area at different time points gradually increased after 60 min post injection and was significantly higher than before injection. Conclusions: Results showed the introduced CT imaging nanoprobe (Au NPs-PEGylated) could be useful for CT imaging of breast tumors under in vivo condition. Overall, it is concluded that Au NPs-PEGylated contrast media is able to detect breast cancer (4T1) cells and is more effective compared with other casual methods. | ||
| کلیدواژهها | ||
| Gold Nanoparticle؛ Polyethylene glycol (PEG)؛ CD24 antibody؛ Computed Tomography؛ 4T1؛ Cancer detection | ||
| مراجع | ||
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