Wear particles enhance autophagy through up-regulation of CD147 to promote osteoclastogenesis | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 6، دوره 21، شماره 8، آبان 2018، صفحه 806-812 اصل مقاله (723.38 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2018.29347.7093 | ||
| نویسندگان | ||
| Baohua Su1؛ Deng Li1؛ Jie Xu1؛ Yingbin Zhang1؛ Zhiqing Cai1؛ Max Daniel Kauther2؛ Ruofan Ma* 1 | ||
| 1Department of Orthopedics, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China, 510120 | ||
| 2Department of Orthopedics and Trauma Surgery, University Hospital Essen, University of Duisburg-Essen, Hufelandstrasse 55, 45147 Essen, Germany | ||
| چکیده | ||
| Objective(s): The study aimed to uncover the underlying mechanism linking wear particles to osteoclast differentiation, and we explored the effect of titanium particles of different sizes on CD147 expression and autophagy in macrophages. Materials and Methods: Effects of titanium particles on CD147 and RANKL mRNA were detected by QPCR; protein level of CD147 and Beclin-1 were detected by Western blot; soluble RANKL were detected by ELISA. To determine the effect of CD147 and autophagy, KG-1a cells were transfected with siRNA-CD147 or treated with autophagy inhibitor CQ (chloroquine), and then co-cultured with different sizes of titanium particles. Results: Our results showed that 0.2-1.2 µm and 1.2-10 µm titanium particles up-regulate CD147 to activate autophagy, which increase the level of soluble RANKL to promote osteoclastogenesis. Suppression of CD147 with siRNA could diminish particle-induced autophagy and soluble RANKL expression. In addition, CQ could dramatically reduce particle-induced soluble RANKL expression. Conclusion: Our findings suggested a possible mechanism underlying wear debris-induced osteolysis and identified CD147 as a potential therapeutic target in aseptic loosening. | ||
| کلیدواژهها | ||
| Autophagy؛ CD147؛ Osteoclastogenesis؛ Peri-implant osteolysis؛ RANKL | ||
| مراجع | ||
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1. Urban RM, Hall DJ, Della Valle C, Wimmer MA, Jacobs JJ, Galante JO. Successful long-term fixation and progression of osteolysis associated with first-generation cementless acetabular components retrieved post mortem. J Bone Joint Surg Am 2012; 94:1877-1885. 2. Athanasou NA. The pathobiology and pathology of aseptic implant failure. Bone Joint Res 2016; 5:162-168. 3. Goodman SB. Wear particles, periprosthetic osteolysis and the immune system. Biomaterials 2007; 28:5044-5048. 4. Hallab NJ, Jacobs JJ. Biologic effects of implant debris. Bull NYU Hosp Jt Dis 2009; 67:182-188. 5. Crotti TN, Smith MD, Findlay DM, Zreiqat H, Ahern MJ, Weedon H, et al. Factors regulating osteoclast formation in human tissues adjacent to peri-implant bone loss: expression of receptor activator NFkappaB, RANK ligand and osteoprotegerin. Biomaterials 2004; 25:565-573. 6. Shen Z, Crotti TN, McHugh KP, Matsuzaki K, Gravallese EM, Bierbaum BE, et al. The role played by cell-substrate interactions in the pathogenesis of osteoclast-mediated peri-implant osteolysis. Arthritis Res Ther 2006; 8:R70. 7. Xing S, Waddell JE, Boynton EL. Changes in macrophage morphology and prolonged cell viability following exposure to polyethylene particulate in vitro. Microsc Res Tech 2002; 57:523-529. 8. Yagil-Kelmer E, Kazmier P, Rahaman MN, Bal BS, Tessman RK, Estes DM. Comparison of the response of primary human blood monocytes and the U937 human monocytic cell line to two different sizes of alumina ceramic particles. J Orthop Res 2004; 22:832-838. 9. Wimhurst JA, Brooks RA, Rushton N. Inflammatory responses of human primary macrophages to particulate bone cements in vitro. J Bone Joint Surg Br 2001; 83:278-282. 10. Ingham E, Green TR, Stone MH, Kowalski R, Watkins N, Fisher J. Production of TNF-alpha and bone resorbing activity by macrophages in response to different types of bone cement particles. Biomaterials 2000; 21:1005-1013. 11. Merkel KD, Erdmann JM, McHugh KP, Abu-Amer Y, Ross FP, Teitelbaum SL. Tumor necrosis factor-alpha mediates orthopedic implant osteolysis. Am J Pathol 1999; 154:203-210. 12. Ingham E, Fisher J. The role of macrophages in osteolysis of total joint replacement. Biomaterials 2005; 26:1271-1286. 13. Nine MJ, Choudhury D, Hee AC, Mootanah R, Osman NAA. Wear debris characterization and corresponding biological response: artificial hip and knee joints. Materials (Basel) 2014; 7:980-1016. 14. Sabokbar A, Fujikawa Y, Neale S, Murray DW, Athanasou NA. Human arthroplasty derived macrophages differentiate into osteoclastic bone resorbing cells. Ann Rheum Dis 1997; 56:414-420. 15. Sabokbar A, Pandey R, Athanasou NA. The effect of particle size and electrical charge on macrophage-osteoclast differentiation and bone resorption. J Mater Sci Mater Med 2003; 14:731-738. 16. Bi Y, Van De Motter RR, Ragab AA, Goldberg VM, Anderson JM, Greenfield EM. Titanium particles stimulate bone resorption by inducing differentiation of murine osteoclasts. J Bone Joint Surg Am 2001; 83-a:501-508. 17. Ren W, Yang SY, Fang HW, Hsu S, Wooley PH. Distinct gene expression of receptor activator of nuclear factor-kappaB and rank ligand in the inflammatory response to variant morphologies of UHMWPE particles. Biomaterials 2003; 24:4819-4826. 18. Childs LM, Paschalis EP, Xing L, Dougall WC, Anderson D, Boskey AL, et al. In vivo RANK signaling blockade using the receptor activator of NF-kappaB: Fc effectively prevents and ameliorates wear debris-induced osteolysis via osteoclast depletion without inhibiting osteogenesis. J Bone Miner Res 2002; 17:192-199. 19. Granchi D, Amato I, Battistelli L, Ciapetti G, Pagani S, Avnet S, et al. Molecular basis of osteoclastogenesis induced by osteoblasts exposed to wear particles. Biomaterials 2005; 26:2371-2379. 20. Xing L, Xiu Y, Boyce BF. Osteoclast fusion and regulation by RANKL-dependent and independent factors. World J Orthop 2012; 3:212-222. 21. Asagiri M, Takayanagi H. The molecular understanding of osteoclast differentiation. Bone 2007; 40:251-264. 22. Maiuri MC, Kroemer G. Autophagy in stress and disease. Cell Death Differ 2015; 22:365-366. 23. Levine B, Klionsky DJ. Development by self-digestion: molecular mechanisms and biological functions of autophagy. Dev Cell 2004; 6:463-477. 24. Cuervo AM. Autophagy and aging: keeping that old broom working. Trends Genet 2008; 24:604-612. 25. Zhang H, Baehrecke EH. Eaten alive: novel insights into autophagy from multicellular model systems. Trends Cell Biol 2015; 25:376-387. 26. Pierrefite-Carle V, Santucci-Darmanin S, Breuil V, Camuzard O, Carle GF. Autophagy in bone: Self-eating to stay in balance. Ageing Res Rev 2015; 24:206-217. 27. Hocking LJ, Whitehouse C, Helfrich MH. Autophagy: a new player in skeletal maintenance?. J Bone Miner Res 2012; 27:1439-1447. 28. Shapiro IM, Layfield R, Lotz M, Settembre C, Whitehouse C. Boning up on autophagy: the role of autophagy in skeletal biology. Autophagy 2014; 10:7-19. 29. Zhao Y, Chen G, Zhang W, Xu N, Zhu JY, Jia J, et al. Autophagy regulates hypoxia-induced osteoclastogenesis through the HIF-1alpha/BNIP3 signaling pathway. J Cell Physiol 2012; 227:639-648. 30. DeSelm CJ, Miller BC, Zou W, Beatty WL, van Meel E, Takahata Y, et al. Autophagy proteins regulate the secretory component of osteoclastic bone resorption. Dev Cell 2011; 21:966-974. 31. Daroszewska A, van’t Hof RJ, Rojas JA, Layfield R, Landao-Basonga E, Rose L, et al. A point mutation in the ubiquitin-associated domain of SQSMT1 is sufficient to cause a Paget’s disease-like disorder in mice. Hum Mol Genet 2011; 20:2734-2744. 32. Lin NY, Chen CW, Kagwiria R, Liang R, Beyer C, Distler A, et al. Inactivation of autophagy ameliorates glucocorticoid-induced and ovariectomy-induced bone loss. Ann Rheum Dis 2016; 75:1203-1210. 33.Wang Z, Liu N, Liu K, Zhou G, Gan J, Wang Z, et al. Autophagy mediated CoCrMo particle-induced peri-implant osteolysis by promoting osteoblast apoptosis. Autophagy 2015; 11:2358-2369. 34. Kanekura T, Miyauchi T, Tashiro M, Muramatsu T. Basigin, a new member of the immunoglobulin superfamily: genes in different mammalian species, glycosylation changes in the molecule from adult organs and possible variation in the N-terminal sequences. Cell Struct Funct 1991; 16:23-30. 35. Huet E, Vallee B, Szul D, Verrecchia F, Mourah S, Jester JV, et al. Extracellular matrix metalloproteinase inducer/CD147 promotes myofibroblast differentiation by inducing alpha-smooth muscle actin expression and collagen gel contraction: implications in tissue remodeling. Faseb j 2008; 22:1144-1154. 36. Gabison EE, Hoang-Xuan T, Mauviel A, Menashi S. EMMPRIN/CD147, an MMP modulator in cancer, development and tissue repair. Biochimie 2005; 87:361-368. 37. Wu B, Cui J, Yang XM, Liu ZY, Song F, Li L, et al. Cytoplasmic fragment of CD147 generated by regulated intramembrane proteolysis contributes to HCC by promoting autophagy. Cell Death Dis 2017; 8:e2925. 38. Rucci N, Millimaggi D, Mari M, Del Fattore A, Bologna M, Teti A, et al. Receptor activator of NF-kappaB ligand enhances breast cancer-induced osteolytic lesions through upregulation of extracellular matrix metalloproteinase inducer/CD147. Cancer Res 2010; 70:6150-6160 39. Nishioku T, Terasawa M, Baba M, Yamauchi A, Kataoka Y. CD147 promotes the formation of functional osteoclasts through NFATc1 signalling. Biochem Biophys Res Commun 2016; 473:620-624. 40. Huang JB, Ding Y, Huang DS, Zeng WK, Guan ZP, Zhang ML. RNA interference targeting p110beta reduces tumor necrosis factor-alpha production in cellular response to wear particles in vitro and osteolysis in vivo. Inflammation 2013; 36:1041-1054. 41. Bu YM, Zheng DZ, Wang L, Liu J. Abrasive endoprosthetic wear particles inhibit IFN-gamma secretion in human monocytes via upregulating TNF-alpha-induced miR-29b. Inflammation 2017; 40:166-173. 42. Purdue PE, Koulouvaris P, Nestor BJ, Sculco TP. The central role of wear debris in periprosthetic osteolysis. Hss j 2006; 2:102-113. 43. Boyce BF, Xing L. Functions of RANKL/RANK/OPG in bone modeling and remodeling. Arch Biochem Biophys 2008; 473:139-146. 44. Green TR, Fisher J, Stone M, Wroblewski BM, Ingham E. Polyethylene particles of a ‘critical size’ are necessary for the induction of cytokines by macrophages in vitro. Biomaterials 1998; 19:2297-2302. 45. Florencio-Silva R, Sasso GR, Simoes MJ, Simoes RS, Baracat MC, Sasso-Cerri E, et al. Osteoporosis and autophagy: What is the relationship?. Rev Assoc Med Bras (1992) 2017; 63:173-179. 46. Fu LL, Cheng Y, Liu B. Beclin-1: autophagic regulator and therapeutic target in cancer. Int J Biochem Cell Biol 2013; 45:921-924. 47. Hussain S, Al-Nsour F, Rice AB, Marshburn J, Yingling B, Ji Z, et al. Cerium dioxide nanoparticles induce apoptosis and autophagy in human peripheral blood monocytes. ACS Nano 2012; 6:5820-5829. 48. Stern ST, Adiseshaiah PP, Crist RM. Autophagy and lysosomal dysfunction as emerging mechanisms of nanomaterial toxicity. Part Fibre Toxicol 2012; 9:20. 49. Nel A, Xia T, Madler L, Li N. Toxic potential of materials at the nanolevel. Science 2006; 311:622-627. 50. Hu Z, Cai M, Deng L, Zhu L, Gao J, Tan M, et al. The fucosylated CD147 enhances the autophagy in epithelial ovarian cancer cells. Oncotarget 2016; 7:82921-82932. 51. Fang F, Wang L, Zhang S, Fang Q, Hao F, Sun Y, et al. CD147 modulates autophagy through the PI3K/Akt/mTOR pathway in human prostate cancer PC-3 cells. Oncol Lett 2015; 9:1439-1443. | ||
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