Effect of Ganoderma lucidum hydroalcoholic extract on insulin release in rat-isolated pancreatic islets | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 5، دوره 2، شماره 4، آذر 2012، صفحه 206-211 اصل مقاله (223.02 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2012.110 | ||
| نویسندگان | ||
| Reza Shafiee-Nick1؛ Seyyed Mohammad Reza Parizadeh* 2؛ Nona Zokaei1؛ Ahmad Ghorbani1 | ||
| 1Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran | ||
| 2Biochemistry and Nutrition Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I. R. Iran | ||
| چکیده | ||
| Objective: Ganoderma Lucidum (G. Lucidum) has been suggested to increase serum insulin level.This study was undertaken to investigateits direct effect on the islets of Langerhans. Material and Methods: Male albino Wistar rats were anesthetized and the islets were isolated after digestion of the pancreas with collagenase. The islets were incubated for 60 min in Krebs bicarbonate buffer containing 3 or 10 mM glucose in the presence of hydroalcoholic extract of G. Lucidum (1 mg/ml), 3-isobutyl-1-methylxanthine (IBMX, 100 µM) or vehicle. Results: Exposure of islets to the extract increased insulin secretion at basal (3 mM) glucose concentration. Increase of glucose concentration to 10 mM resulted in a significant increase in the rate of insulin secretion. While the IBMX could augment insulin release evoked by 10 mM glucose, the extract failed to modify it. Conclusion: Our results demonstrate that G. lucidum acts directly on the Langerhans islets to increase basal insulin release. | ||
| کلیدواژهها | ||
| Ganoderma lucidum؛ Insulin؛ Isolated islets؛ Rat | ||
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