Protective effect of Rheum turkestanicum root against mercuric chloride-induced hepatorenal toxicity in rats | ||
| Avicenna Journal of Phytomedicine | ||
| مقاله 3، دوره 8، شماره 6، بهمن و اسفند 2018، صفحه 488-497 اصل مقاله (864.64 K) | ||
| نوع مقاله: Original Research Article | ||
| شناسه دیجیتال (DOI): 10.22038/ajp.2018.29651.2034 | ||
| نویسندگان | ||
| Azar Hosseini1؛ Arezoo Rajabian2؛ Sahar Fanoudi2؛ Mehdi Farzadnia3؛ Mohammad Taher Boroushaki* 1 | ||
| 1Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 2Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 3Cancer Molecular Pathology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| چکیده | ||
| Objective: The present study was designed to investigate the protective effects of hydroalcoholic extract of Rheum turkestanicum against HgCl2 hepatorenal toxicity in rats. Materials and Methods: Animals were randomly divided into five groups (n= 6 in each group) and received HgCl2 and plant’s extract, intraperitoneally. Group1 received saline (1 mL/kg/day), group 2 received extract (200 mg/kg/day), group 3 was treated with HgCl2 (5 mg/kg/day,) and groups 4 and 5 received the extract (100 and 200 mg/kg/day, respectively), 1 hr before HgCl2 administration. All injections last for 3 days. Blood samples and specimens of the liver and kidney were collected 24 hr after the last injection. Results: Data showed that HgCl2 significantly increases liver malondialdehyde (MDA) level, reduces total sulfhydryl content and increases serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity, compared to control group. The histopathological changes such as inflammatory cells infiltration was observed in HgCl2-treated group while plant’s extract partially improved histological changes. The extract (100 and 200 mg/kg/day) improved the liver functions as reflected by significant reductions in AST and ALT levels in serum, MDA decreased and the content of total sulfhydryl elevated. Also, the extract improved necrosis and atrophy of the kidney induced byHgCl2. Pretreatment with the extract reduced creatinine and urea in serum, and glucose and protein concentrations in urine, compared to HgCl2- treated group (group III). The extract significantly reversed HgCl2-induced depletion in thiol content and elevation in MDA content. Conclusion: Therefore, oxidative stress may play an important role in HgCl2-induced hepatorenal injury and R. turkestanicum extract may be regarded as a useful to protect the kidney and liver against HgCl2-induced oxidative damage. | ||
| کلیدواژهها | ||
| HgCl2؛ Oxidative stress؛ Rheum turkestanicum, Lipid peroxidation | ||
| مراجع | ||
|
Adeneye A and Olagunju J. 2008. Protective effect of oral ascorbic acid against acetaminophen induced-hepatic injury in rats. Afr J Biomed Res, 11: 183 -190.
Agarwal R, Goel SK, Behari JR. 2010a. Detoxification and antioxidant effects of curcumin in rats experimentally exposed to mercury. J Appl Toxicol, 30: 457-468.
Agarwal R, Goel SK, Chandra R. 2010b. Role of vitamin E in preventing acute mercury toxicity in rat. Environ Toxicol Pharmacol, 29: 70-78.
Alam MM, Javed K and Jafri MA. 2005. Effect of Rheum emodi (Revand Hindi) on renal functions in rats. J Ethnopharmacol, 96: 121-125.
Andiç S, Tunçtürk Y, Ocak E, Kose S. 2009. Some chemical characteristics of edible wild Rhubarb species (Rheum ribes L.). Res J Agric Biol Sci, 5: 973-977.
Boroushaki MT, Asadpour E, Sadeghnia HR, Dolati K. 2014a. Effect of pomegranate seed oil against gentamicin -induced nephrotoxicity in rat. J Food Sci Technol, 51: 3510-3514.
Boroushaki MT, Hosseini A, Dolati K, Mollazadeh H, Rajabian A. 2016. Protective effect of pomegranate seed oil against mercuric chloride-induced hepatotoxicity in rat. Acta Poloniae Pharmaceutica Drug Res, 73: 991-997.
Boroushaki MT, Mollazadeh H, Rajabian A, Dolati K, Hoseini A, Paseban M, Farzadnia M. 2014b. Protective effect of pomegranate seed oil against mercuric chloride-induced nephrotoxicity in rat. Ren Fail, 36: 1581-1586.
Bouroshaki MT, Sadeghnia HR, Banihasan M, Yavari S. 2010. Protective effect of pomegranate seed oil on hexachlorobutadiene-induced nephrotoxicity in rat kidneys. Ren Fail, 32: 612-617.
Chai YY, Wang F, Li YL, Liu K, Xu H. 2012. Antioxidant Activities of Stilbenoids from Rheum emodi Wall. Evid Based Complement Alternat Med, 2012: 603678.
Clarkson TW. 1997. The toxicology of mercury. Crit Rev Clin Lab Sci, 34: 369-403.
Dorsey JF and Kao GD. 2007. Aloe (-emodin) for cancer? More than just a comforting salve. Cancer Biol Ther, 6: 89-90.
Durak D, Kalender S, Uzun FG, Demyr F, Kalender Y. 2010. Mercury chloride-induced oxidative stress in human erythrocytes and the effect of vitamins C and E in vitro. African J Biotechnol 9:488-495.
Ebrahimzadeh MA and Bahramian F. 2009. Antioxidant activity of Crataegus pentaegyna subsp. elburensis fruits extracts used in traditional medicine in Iran. Pak J Biol Sci, 12: 413-419.
El-Shenawy SM and Hassan NS. 2008. Comparative evaluation of the protective effect of selenium and garlic against liver and kidney damage induced by mercury chloride in the rats. Pharmacol Rep, 60: 199-208.
Emanuelli T, Rocha JB, Pereira ME, Porciuncula LO, Morsch VM, Martins AF, Souza DO. 1996. Effect of mercuric chloride intoxication and dimercaprol treatment on delta-aminolevulinate dehydratase from brain, liver and kidney of adult mice. Pharmacol Toxicol, 79: 136-143.
Gstraunthaler G, Pfaller W and Kotanko P. 1983. Glutathione depletion and in vitro lipid peroxidation in mercury or maleate induced acute renal failure. Biochem pharmacol, 32: 2969-2972.
Hamzeh S, Farokhi F, Heydari R, Manaffar R. 2014. Renoprotective effect of hydroalcoholic extract of Rheum ribes root in diabetic female rats. Avicenna J Phytomed, 4: 392-401.
Hosseini A, Mollazadeh H, Amiri MS, Sadeghnia HR, Ghorbani A. 2017. Effects of a standardized extract of Rheum turkestanicum Janischew root on diabetic changes in the kidney, liver and heart of streptozotocin-induced diabetic rats. Biomed Pharmacother, 86: 605-611.
Hosseini A and Rajabian A. 2016. Protective effect of Rheum turkestanikum root against doxorubicin-induced toxicity in H9c2 cells. Revista Brasileira de Farmacog, 26: 347-351.
Hosseinzadeh H, Sadeghnia HR, Ziaee T, Danaee A. 2005. Protective effect of aqueous saffron extract (Crocus sativus L.) and crocin, its active constituent, on renal ischemia-reperfusion-induced oxidative damage in rats. J Pharm Pharm Sci, 8: 387-393.
Huang Y, Cheng S and Lin T. 1996. Lipid peroxidation in rats administrated with mercuric chloride. Biol Trace Element Res, 52: 193.
Kalender S, Uzun FG, Demir F, Uzunhisarcıklı M, Aslanturk A. 2013. Mercuric chloride-induced testicular toxicity in rats and the protective role of sodium selenite and vitamin E. Food Chem Toxicol, 55: 456-462.
Li X, Zhang J, Liu W, Li X, Zhang X, Jiang Y, Zhou J, Jin Y. 2011. Serum levels of perfluorinated compounds in the general population in Shenzhen, China Chinese Sci Bull, 56: 3092-3099.
Linden A, Gülden M, Martin H-J,Maser E, Seibert H.. 2008. Peroxide-induced cell death and lipid peroxidation in C6 glioma cells. Toxicol in Vitro, 22: 1371-1376.
Liu H, Gu LB, Tu Y, Hu H, Huang YR, Sun W. 2016. Emodin ameliorates cisplatin-induced apoptosis of rat renal tubular cells in vitro by activating autophagy. Acta Pharmacol Sin, 37: 235-245.
Lott JA and Turner K. 1975. Evaluation of Trinder's glucose oxidase method for measuring glucose in serum and urine. Clin Chem, 21: 1754-1760.
Mahboob M, Shireen KF, Atkinson A,Khan AT. 2001. Lipid peroxidation and antioxidant enzyme activity in different organs of mice exposed to low level of mercury. J Environ Sci Health, Part B 36: 687-697.
Masson P, Ohlsson P and Bjorkhem I. 1981. Combined enzymatic – Jaffe’s method for determination of creatinine in serum. Clin Chem, 27: 18-21.
McElderry L, Tarbit I and Cassells-Smith A. 1982. Six methods for urinary protein compared. Clin Chem, 28: 356-360.
Miller DM, Lund BO and Woods JS. 1991. Reactivity of Hg (II) with superoxide: evidence for the catalytic dismutation of superoxide by Hg(II). J Biochem Toxicol, 6: 293-298.
Moraes-Silva L, Siqueira LF, Oliveira VA, Oliveira CS, Ineu RP, Pedroso TF, Fonseca MM, Pereira ME. 2014. Preventive effect of CuCl(2) on behavioral alterations and mercury accumulation in central nervous system induced by HgCl2 in newborn rats. J Biochem Mol Toxicol, 28: 328-335.
Nava M, Romero F, Quiroz Y,Parra G, Bonet L, Rodríguez-Iturbe B.. 2000. Melatonin attenuates acute renal failure and oxidative stress induced by mercuric chloride in rats. Am J Physiol Renal Physiol,279: F910-918.
Nogueira CW, Soares FA, Nascimento PC, Muller D, Rocha JB. 2003. 2, 3-Dimercaptopropane-1-sulfonic acid and meso-2, 3-dimercaptosuccinic acid increase mercury- and cadmium-induced inhibition of delta-aminolevulinate dehydratase. Toxicol 184: 85-95.
Oda SS and El-Ashmawy IM. 2012. Protective effect of silymarin on mercury-induced acute nephro-hepatotoxicity in rats. Glob Vet, 9: 376-383.
Patrick L. 2002. Mercury toxicity and antioxidants: Part 1: role of glutathione and alpha-lipoic acid in the treatment of mercury toxicity. Altern Med Rev, 7: 456-471.
Pillai A and Gupta S. 2005. Antioxidant enzyme activity and lipid peroxidation in liver of female rats co-exposed to lead and cadmium: effects of vitamin E and Mn2+. Free Radic Res, 39: 707-712.
Rozgaj R, Kasuba V and Blanusa M. 2005. Mercury chloride genotoxicity in rats following oral exposure, evaluated by comet assay and micronucleus test. Arh Hig Rada Toksikol, 56: 9-15.
Şener G, Şehirli AÖ and Ayanogˇlu‐Dülger G. 2003. Melatonin Protects against mercury (II) ‐induced oxidative tissue damage in rats. Pharmacol Toxicol, 93: 290-296.
Sharma MK, Sharma A, Kumar A, Kumar M. 2007. Spirulina fusiformis provides protection against mercuric chloride induced oxidative stress in Swiss albino mice. Food Chem Toxicol, 45: 2412-2419.
Sridhar MPN, Nandakumar N, Rengarajan T, Balasubramanian MP. 2013. Amelioration of mercuric chloride induced oxidative stress by Hygrophila auriculata (K. Schum) Heine via modulating the oxidant-antioxidant imbalance in rat liver. J Biochem Technol, 4: 622-627.
Stohs S and Bagchi D. (1995) Oxidative mechanisms in the toxicity of metal ions. Free Radic Biol Med, 18: 321-336.
Tanaka-Kagawa T, Suzuki M, Naganuma A, Yamanaka N, Imura N. 1998, Strain difference in sensitivity of mice to renal toxicity of inorganic mercury. J Pharmacol Exp Ther, 285: 335-341.
Van Acker SA, Tromp MN, Griffioen DH, Van Bennekom WP, van der Vijgh WJ, Bast A. 1996. Structural aspects of antioxidant activity of flavonoids. FreeRadic Biol Med, 20: 331-342.
Zalups RK. 2000. Molecular interactions with mercury in the kidney. Pharmacol Rev, 52: 113-144.
Zhang R, Kang KA, Piao MJ, Lee KH, Jang HS, Park MJ, Kim BJ, Kim JS, Kim YS, Ryu SY, Hyun JW. 2007.Rhapontigenin from Rheum undulatum protects against oxidative-stress-induced cell damage through antioxidant activity. J Toxicol Environ Health A, 70: 1155-1166.
Zhen-kui Q, Zhuo-ran Z, Li-zhi T, WANG K, ZHANG R.2014. Protective effect of emodin on ischemia reperfusion injury of kidney autotransplantation. Drug and Clin.
| ||
|
آمار تعداد مشاهده مقاله: 26,071 تعداد دریافت فایل اصل مقاله: 12,173 |
||