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Malekinejad, Hassan, Bazargani-Gilani, Behnaz, Tukmechi, Amir, Ebrahimi, Hadi. (1391). A cytotoxicity and comparative antibacterial study on the effect of Zataria multiflora Boiss, Trachyspermum copticum essential oils, and Enrofloxacin on Aeromonas hydrophila. , 2(4), 188-195. doi: 10.22038/ajp.2012.108
Hassan Malekinejad; Behnaz Bazargani-Gilani; Amir Tukmechi; Hadi Ebrahimi. "A cytotoxicity and comparative antibacterial study on the effect of Zataria multiflora Boiss, Trachyspermum copticum essential oils, and Enrofloxacin on Aeromonas hydrophila". , 2, 4, 1391, 188-195. doi: 10.22038/ajp.2012.108
Malekinejad, Hassan, Bazargani-Gilani, Behnaz, Tukmechi, Amir, Ebrahimi, Hadi. (1391). 'A cytotoxicity and comparative antibacterial study on the effect of Zataria multiflora Boiss, Trachyspermum copticum essential oils, and Enrofloxacin on Aeromonas hydrophila', , 2(4), pp. 188-195. doi: 10.22038/ajp.2012.108
Malekinejad, Hassan, Bazargani-Gilani, Behnaz, Tukmechi, Amir, Ebrahimi, Hadi. A cytotoxicity and comparative antibacterial study on the effect of Zataria multiflora Boiss, Trachyspermum copticum essential oils, and Enrofloxacin on Aeromonas hydrophila. , 1391; 2(4): 188-195. doi: 10.22038/ajp.2012.108

A cytotoxicity and comparative antibacterial study on the effect of Zataria multiflora Boiss, Trachyspermum copticum essential oils, and Enrofloxacin on Aeromonas hydrophila

Avicenna Journal of Phytomedicine
مقاله 3، دوره 2، شماره 4، آذر 2012، صفحه 188-195    اصل مقاله (252.44 K)
نوع مقاله: Original Research Article
شناسه دیجیتال (DOI): 10.22038/ajp.2012.108
نویسندگان
Hassan Malekinejad* 1؛ Behnaz Bazargani-Gilani1؛ Amir Tukmechi2؛ Hadi Ebrahimi1
1Department of Pharmacology & Toxicology, Faculty of Veterinary Medicine, Urmia University, Urmia, I. R. Iran
2Department of Pathobiology & Quality Control, Artemia & Aquatic Animal Research Institute, Urmia University, Urmia, I. R. Iran
چکیده
Objective: this study designed to test the antibacterial potency of enrofloxacin (ENR) and essential oils from Zataria multiflora Boiss (ZEO) and Trachyspermum copticum (TEO)on Aeromonas hydrophila.
Material and Methods: The antibacterial potency of test compounds was determined by several methods including the inhibition zone diameter determination, microbroth dilution method and colorimetric method of MTT. The cytotoxicity of test substances was assessed on Chinook salmon (Oncorhynchus tshawytscha) embryo (CHSE-214) cells.
Results: Results showed that ENR and tested essential oils exert antibacterial effect against A. hydrophila. Moreover, ENR exerted the most potent antibacterial effect with MIC values of 62.5 ng/ml. The natural compounds of ZEO and TEO also showed antibacterial effects with rather high MIC values of 0.315 mg/ml, and 1.25 mg/ml, respectively. None of the tested substances showed toxicity on CHSE-24 cells.
Conclusion: It is concluded that ZEO and TEO could be applied to prevent from A. hydrophila infection. Moreover, data also suggest that MTT method could be both cost- and time-effective and accurate method of MIC determination.
کلیدواژه‌ها
Natural Antibacterial Substances؛ Microbroth dilution Method؛ MTT Technique؛ Cytotoxicity
آمار
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