Liu, Zhigang, Huang, Shuwei, Hu, Pengfei, Zhou, Hui. (1397). The role of autophagy in advanced glycation end product-induced proliferation and migration in rat vascular smooth muscle cells. , 21(6), 634-638. doi: 10.22038/ijbms.2018.20266.5305
Zhigang Liu; Shuwei Huang; Pengfei Hu; Hui Zhou. "The role of autophagy in advanced glycation end product-induced proliferation and migration in rat vascular smooth muscle cells". , 21, 6, 1397, 634-638. doi: 10.22038/ijbms.2018.20266.5305
Liu, Zhigang, Huang, Shuwei, Hu, Pengfei, Zhou, Hui. (1397). 'The role of autophagy in advanced glycation end product-induced proliferation and migration in rat vascular smooth muscle cells', , 21(6), pp. 634-638. doi: 10.22038/ijbms.2018.20266.5305
Liu, Zhigang, Huang, Shuwei, Hu, Pengfei, Zhou, Hui. The role of autophagy in advanced glycation end product-induced proliferation and migration in rat vascular smooth muscle cells. , 1397; 21(6): 634-638. doi: 10.22038/ijbms.2018.20266.5305

The role of autophagy in advanced glycation end product-induced proliferation and migration in rat vascular smooth muscle cells

Iranian Journal of Basic Medical Sciences
مقاله 13، دوره 21، شماره 6، شهریور 2018، صفحه 634-638    اصل مقاله (939.1 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2018.20266.5305
نویسندگان
Zhigang Liu1؛ Shuwei Huang2؛ Pengfei Hu2؛ Hui Zhou* 3
1Department of Cardiology, Yidu Central Hospital of Weifang, Qingzhou, Shandong, 262500, China
2Department of Cardiology, Second Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310053, China
3Department of Cardiology, Zhejiang hospital, Hangzhou, 310013), China
چکیده
Objective(s): To investigate the role of autophagy in advanced glycation end products (AGEs)-induced proliferation and migration in rat vascular smooth muscle cells (VSMCs).
Materials and Methods: After culture, VSMCs were treated with 0, 1, 10, and 100 μg/ml concentrations of AGEs. Autophagy specific protein light chain 3 (LC3)-I/II was determined by western blotting, autophagosomes were observed with electron microscopy, cell proliferation was quantified using the methyl thiazolyl tetrazolium (MTT) assay, and cell migration was evaluated using Transwell migration and scratch assays.
Results: Compared to the control group, the level of LC3- II/I in AGEs treatment group was up-regulated, and the number of autophagosomes was also increased. Furthermore, in concentration of 100 μg/ml AGEs, the extent of proliferation and migration was significantly increased compared to the control group. However, pretreating cells with autophagy inhibitor 3-MA could attenuate these effects.
Conclusion: Our study demonstrated that AGEs-induced autophagy accelerated AGEs-stimulated proliferation and migration in VSMCs.
کلیدواژه‌ها
Advanced Glycation End Products؛ Autophagy؛ Cell Proliferation؛ Muscle؛ Smooth؛ Transcellular cell migration؛ Vascular
مراجع
آمار
تعداد مشاهده مقاله: 1,205
تعداد دریافت فایل اصل مقاله: 816