Gestational diabetes influences the expression of hypertrophic genes in left ventricle of rat’s offspring | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 13، دوره 21، شماره 5، مرداد 2018، صفحه 525-528 اصل مقاله (569.9 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2018.25116.6233 | ||
| نویسندگان | ||
| Elia Saragard Kermani1؛ Zahra Nazari2؛ Mehdi Mehdizadeh3؛ Majid Shahbazi4؛ Mohammad Jafar Golalipour* 5 | ||
| 1Department of Anatomical sciences, Golestan University of Medical Sciences, Gorgan, Iran | ||
| 2Gorgan Congenital Malformations research Center, Golestan University of Medical Sciences, Gorgan, Iran | ||
| 3Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran | ||
| 4Molecular Genetic Research Center, Golestan University of Medical Sciences, Gorgan, Iran | ||
| 5Gorgan Congenital Malformations Research Center, Department of Anatomical sciences, Golestan University of Medical Sciences, Gorgan, Iran | ||
| چکیده | ||
| Objective(s): Gestational diabetes increases the risk of congenital heart disease in the offspring, but the molecular mechanism underlying this process remains unclear. Therefore, the current study was conducted to assess the effects of induced gestational diabetes on expression of some involved genes in cardiac hypertrophy in the offspring of diabetic rats. Materials and Methods: Diabetes was induced in 40 adult Wistar rats by intraperitoneal injection of 45 mg/kg of streptozotocin. The day of appearance of the vaginal plug was assumed as day zero of gestation for inducing diabetes. After pregnancy, the offspring was maintained until they reach the age of 12 weeks. Then, their hearts were excised and were sectioned for molecular study. We analyzed the expression pattern of some hypertrophic genes by the quantitative real-time RT-PCR. Results: The mRNA expression levels of all studied genes including c-jun, c-fos, c-myc, alpha-myosin heavy chain (α-MHC), atrial natriuretic factor (ANF) and β-MHC, which are important in cardiomyocyte hypertrophy, were higher in the offspring of the diabetic group compared to controls. Significant differences were found for β-MHC and c-myc with PConclusion: Gestational diabetes upregulates expression of c-jun, c-fos c-myc, α-MHC, ANF and β-MHC genes that are involved in cardiac hypertrophy in the offspring of diabetic rats. | ||
| کلیدواژهها | ||
| Gestational diabetes؛ Heart؛ Hypertrophic genes Offspring؛ Rats | ||
| مراجع | ||
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