An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 4، دوره 21، شماره 5، مرداد 2018، صفحه 465-468 اصل مقاله (423.98 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2018.26152.6420 | ||
| نویسندگان | ||
| Elif Iplik1؛ Baris Ertugrul2؛ İlknur Kozanoglu3؛ Yusuf Baran4، 5؛ Bedia Cakmakoglu* 2 | ||
| 1Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Istanbul Yeni Yuzyil University, Istanbul, Turkey | ||
| 2Department of Molecular Medicine, Aziz Sancar Institute for Experimental Medicine, Istanbul University, Istanbul, Turkey | ||
| 3Department of Hematology, Baskent University, Adana, Turkey | ||
| 4Faculty of Life and Natural Sciences, Abdullah Gul University, Kayseri, Turkey | ||
| 5Department of Molecular Biology and Genetics, Izmir Institute of Technology, Urla, Izmir, Turkey | ||
| چکیده | ||
| Objective(s): Colon cancer is risen up with its complex mechanism that directly impacts on its treatment as well as its common prevalence. Mesenchymal stem cells (MSCs) have been considered as a therapeutic candidate for conventional disease including cancer. In this research, we have focused on apoptotic effects of adipose tissue-derived MSCs in colon cancer. Materials and Methods: MSCs were obtained from adipose tissue and characterized by Flowcytometer using suitable antibodies. MSCs, HT-29, HCT-116, RKO and healthy cell line MRC5 were cultured by different seeding procedure. After cell viability assay, changes in caspase 3 enzyme activity and the level of phosphatidylserine were measured. Results: For cell viability assay, a 48 hr incubation period was chosen to seed all cells together. There was a 1.36-fold decrease in caspase 3 enzyme activity by co-treatment of RKO and MSCs in addition to 2.02-fold decrease in HT-29 and MSCs co-treatment, and 1.103-fold increase in HCT-116 and MSCs. The results demonstrated that HCT-116 led to the highest rate of apoptotic cell death (7.5%) compared with other cells. Conclusion: We suggest that MSCs might remain a new treatment option for cancer by its differentiation and repair capacity. | ||
| کلیدواژهها | ||
| Apoptosis؛ Colon cancer؛ Stem cells؛ Cell death | ||
| مراجع | ||
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