Iplik, Elif, Ertugrul, Baris, Kozanoglu, İlknur, Baran, Yusuf, Cakmakoglu, Bedia. (1397). An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue. , 21(5), 465-468. doi: 10.22038/ijbms.2018.26152.6420
Elif Iplik; Baris Ertugrul; İlknur Kozanoglu; Yusuf Baran; Bedia Cakmakoglu. "An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue". , 21, 5, 1397, 465-468. doi: 10.22038/ijbms.2018.26152.6420
Iplik, Elif, Ertugrul, Baris, Kozanoglu, İlknur, Baran, Yusuf, Cakmakoglu, Bedia. (1397). 'An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue', , 21(5), pp. 465-468. doi: 10.22038/ijbms.2018.26152.6420
Iplik, Elif, Ertugrul, Baris, Kozanoglu, İlknur, Baran, Yusuf, Cakmakoglu, Bedia. An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue. , 1397; 21(5): 465-468. doi: 10.22038/ijbms.2018.26152.6420

An answer to colon cancer treatment by mesenchymal stem cell originated from adipose tissue

Iranian Journal of Basic Medical Sciences
مقاله 4، دوره 21، شماره 5، مرداد 2018، صفحه 465-468    اصل مقاله (423.98 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2018.26152.6420
نویسندگان
Elif Iplik1؛ Baris Ertugrul2؛ İlknur Kozanoglu3؛ Yusuf Baran4، 5؛ Bedia Cakmakoglu* 2
1Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Istanbul Yeni Yuzyil University, Istanbul, Turkey
2Department of Molecular Medicine, Aziz Sancar Institute for Experimental Medicine, Istanbul University, Istanbul, Turkey
3Department of Hematology, Baskent University, Adana, Turkey
4Faculty of Life and Natural Sciences, Abdullah Gul University, Kayseri, Turkey
5Department of Molecular Biology and Genetics, Izmir Institute of Technology, Urla, Izmir, Turkey
چکیده
Objective(s): Colon cancer is risen up with its complex mechanism that directly impacts on its treatment as well as its common prevalence. Mesenchymal stem cells (MSCs) have been considered as a therapeutic candidate for conventional disease including cancer. In this research, we have focused on apoptotic effects of adipose tissue-derived MSCs in colon cancer.
Materials and Methods: MSCs were obtained from adipose tissue and characterized by Flowcytometer using suitable antibodies. MSCs, HT-29, HCT-116, RKO and healthy cell line MRC5 were cultured by different seeding procedure. After cell viability assay, changes in caspase 3 enzyme activity and the level of phosphatidylserine were measured.
Results: For cell viability assay, a 48 hr incubation period was chosen to seed all cells together. There was a 1.36-fold decrease in caspase 3 enzyme activity by co-treatment of RKO and MSCs in addition to 2.02-fold decrease in HT-29 and MSCs co-treatment, and 1.103-fold increase in HCT-116 and MSCs. The results demonstrated that HCT-116 led to the highest rate of apoptotic cell death (7.5%) compared with other cells.
Conclusion: We suggest that MSCs might remain a new treatment option for cancer by its differentiation and repair capacity.
کلیدواژه‌ها
Apoptosis؛ Colon cancer؛ Stem cells؛ Cell death
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آمار
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