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Banihahsemi, Seyed Reza, Zavaran Hosseini, Ahmad, Rahbarizadeh, Fatemeh, Ahmadvand, Davoud. (1397). Development of specific nanobodies (VHH) for CD19 immuno-targeting of human B-lymphocytes. , 21(5), 455-464. doi: 10.22038/ijbms.2018.26778.6557
Seyed Reza Banihahsemi; Ahmad Zavaran Hosseini; Fatemeh Rahbarizadeh; Davoud Ahmadvand. "Development of specific nanobodies (VHH) for CD19 immuno-targeting of human B-lymphocytes". , 21, 5, 1397, 455-464. doi: 10.22038/ijbms.2018.26778.6557
Banihahsemi, Seyed Reza, Zavaran Hosseini, Ahmad, Rahbarizadeh, Fatemeh, Ahmadvand, Davoud. (1397). 'Development of specific nanobodies (VHH) for CD19 immuno-targeting of human B-lymphocytes', , 21(5), pp. 455-464. doi: 10.22038/ijbms.2018.26778.6557
Banihahsemi, Seyed Reza, Zavaran Hosseini, Ahmad, Rahbarizadeh, Fatemeh, Ahmadvand, Davoud. Development of specific nanobodies (VHH) for CD19 immuno-targeting of human B-lymphocytes. , 1397; 21(5): 455-464. doi: 10.22038/ijbms.2018.26778.6557

Development of specific nanobodies (VHH) for CD19 immuno-targeting of human B-lymphocytes

Iranian Journal of Basic Medical Sciences
مقاله 3، دوره 21، شماره 5، مرداد 2018، صفحه 455-464    اصل مقاله (949.23 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2018.26778.6557
نویسندگان
Seyed Reza Banihahsemi1؛ Ahmad Zavaran Hosseini* 1؛ Fatemeh Rahbarizadeh2؛ Davoud Ahmadvand3
1Department of Medical Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3Department of Medical Biochemistry, Faculty of Applied Medical Sciences, Iran University of Medical Sciences,Tehran, Iran
چکیده
Objective(s): CD19 is a transmembrane glycoprotein of immunoglobulin superfamily. In order to treat lymphoma, monoclonal antibodies (mAb) can target different antigens, including CD19, CD20 and CD22 on the surface of B-cells. Along with biotechnology progress, a new generation of antibodies is introduced, with the purpose of eliminating the defects of the previous generation. Among the most developed one are nanobodies (Nb).  Nbs are a unique kind of camelid single domain antibody fragments with a broad range of medical applications. Unique physicochemical properties of Nbs have made them ideal candidates for therapeutic and diagnostic applications.
Materials and Methods: An immune gene library was created, and several CD19 specific Nbs were selected through antigen panning process, and their molecular properties as well as specificity, sensitivity, affinity and immunoreactivity against CD19 positive and negative cells were evaluated.
Results: The Nb library was prepared with 7.2 x107 members. We managed to isolate a panel of CD19-specific Nbs after the last round of selection with the affinity of isolated Nbs being estimated at the standard range of 15-35 nM.  Sequence analysis of positive clones was indicative of the fact that 12 variable sequences were confirmed. Of all these 12 clones, 2 clones with the greatest level signal in ELISA underwent subsequent analysis. Our sequencing results indicated high sequence homology (approximately 90%) between the Nb and Homa variable immunoglobulin domains.
Conclusion: Specific Nbs possess the potential to be used as novel therapeutic approaches in order to treat autoimmune diseases and B-cell lymphoma.
کلیدواژه‌ها
CD19؛ Drug Delivery system؛ Lymphoma؛ Nanobody؛ Phage display
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