High Serum Alpha-2-Macroglobulin Level in Patients with Osteonecrosis of the Femoral Head | ||
The Archives of Bone and Joint Surgery | ||
مقاله 9، دوره 6، شماره 3، مرداد 2018، صفحه 219-224 اصل مقاله (519.47 K) | ||
نوع مقاله: RESEARCH PAPER | ||
شناسه دیجیتال (DOI): 10.22038/abjs.2017.25310.1668 | ||
نویسندگان | ||
Zari Naderi Ghale-Noie1؛ Mohammad Hassani2؛ Amir R. Kachooei3؛ Mohammad A. Kerachian* 4 | ||
1Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Department of Medical Genetics, Mashhad University of Medical Sciences, Mashhad, Iran | ||
2Department of Orthopedic Surgery, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran | ||
3Orthopedic Research Center, Mashhad University of Medical Sciences, Mashhad, Iran | ||
4Department of Medical Genetics, Mashhad University of Medical Sciences, Mashhad, Iran | ||
چکیده | ||
Background: Diagnosis of osteonecrosis of the femoral head (ONFH) is complicated due to the lack of reliable serum biomarkers. Up-regulation of alpha-2-macroglobulin (A2M) gene has been reported in glucocorticoid-induced ANFH rat model. This study aimed to investigate whether the serum level of alpha-2-macroglobulin (A2M) can be used for ONFH diagnosis. Methods: Serum protein capillary electrophoresis was performed on the sera of 36 ONFH patients. Also, human enzyme-linked immunosorbent assay was performed to evaluate the serum levels of A2M. Results: Alpha-2 subunit level, composed of alpha-2-macroglobulin, ceruloplasmin and 2-2 haptoglobin phenotype, was increased significantly as compared to healthy subjects (P=0.0001). Moreover, ELISA assay confirmed significant elevation in the A2M (P=0.037). Conclusion: Our findings suggest that avascular necrotic femur head presumably directly or indirectly elevates A2M in the bloodstream. Thus, serum level of A2M might be used as a reliable diagnostic tool in clinical practice. Level of evidence: II | ||
کلیدواژهها | ||
Alpha-2-macroglobulin؛ Avascular necrosis of femoral head؛ Femur؛ Osteonecrosis | ||
مراجع | ||
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