Comparison of the efficacy of Piascledine and transforming growth factor β1 on chondrogenic differentiation of human adipose-derived stem cells in fibrin and fibrin-alginate scaffolds | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 14، دوره 21، شماره 2، اردیبهشت 2018، صفحه 212-218 اصل مقاله (977.12 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2018.24693.6136 | ||
| نویسندگان | ||
| Batul Hashemibeni1؛ Ali Valiani1؛ Mojtaba Esmaeili1؛ Mohamad Kazemi2؛ Maryam Aliakbari1؛ Farhad Golshan Iranpour* 1 | ||
| 1Department of Anatomical Sciences, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| 2Department of Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran | ||
| چکیده | ||
| Objective(s):The aim of this study was to compare the chondrogenic induction potential of Piascledine and TGF-β1 on adipose-derived stem cells (ADSCs) in fibrin and fibrin-alginate scaffolds. Materials and Methods: Human subcutaneous adipose tissues were harvested from three patients who were scheduled to undergo liposuction. Isolated ADSCs were proliferated in a culture medium. Then, the cells were seeded in fibrin or fibrin-alginate scaffolds and cultured for 14 days in a chondrogenic medium containing Piascledine, TGF-β1, or both. The rate of cell proliferation and survival was evaluated by using MTT [3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide] assay and the rate of the expression of type II collagen, aggrecan, and type X collagen genes was evaluated by real-time polymerase chain reaction (real-time PCR) method. Results: The MTT results showed that Piascledine is able to enhance the proliferation and survival of ADSCs in fibrin scaffolds in comparison to other groups (P<0.05). Real-time PCR evaluation revealed that the expression of type II collagen was higher in TGF- β1groups, but the expression of aggrecan was higher in TGF-β1 alone or along with Piascledine in fibrin-alginate scaffolds. Furthermore, the expression of type X collagen was lower in Piascledine alone or along with TGF-β1 in fibrin scaffold. Conclusion: Piascledine can enhance the proliferation and differentiation of ADSCs in fibrin scaffolds. | ||
| کلیدواژهها | ||
| Chondrogenesis Piascledine؛ Stem cells؛ Tissue engineering؛ Transforming growth-؛ factor beta 1 | ||
| مراجع | ||
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